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Cand1 (NM_027994) Mouse Tagged ORF Clone

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SKU
MG216867
Cand1 (tGFP-tagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,806.00
6 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cand1
Synonyms 2310038O07Rik; 6330512O03Rik; AI195005; AI846556; D10Ertd516e; mKIAA0829; Tp120a
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216867 representing NM_027994
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAGCGCTTCGTACCACATCTCCAACTTGCTGGAGAAAATGACATCCAGCGACAAGGACTTCAGGT
TCATGGCGACCAACGACCTGATGACAGAGCTGCAGAAAGACTCTATCAAGCTGGACGATGACAGCGAGAG
GAAAGTTGTGAAGATGATTCTCAGGTTGCTGGAGGATAAAAATGGCGAAGTGCAGAACTTAGCTGTCAAA
TGCCTTGGCCCTTTGGTGAGTAAAGTGAAAGAGTACCAAGTGGAGACGATCGTGGACACCCTCTGCACCA
ACATGCTTTCTGACAAGGAGCAGCTGCGAGACATTTCCAGCATCGGCCTGAAAACAGTAATTGGAGAACT
TCCGCCAGCTTCCAGTGGCTCTGCATTAGCTGCTAATGTATGTAAAAAGATTACTGGACGCCTTACCAGT
GCAATAGCAAAGCAGGAAGATGTTTCTGTTCAGCTCGAAGCCTTGGATATCATGGCTGACATGTTGAGCA
GGCAAGGAGGACTGCTTGTTAATTTCCACCCTTCAATTCTGACCTGTCTGCTTCCCCAGCTGACCAGCCC
TAGACTTGCAGTGAGGAAAAGAACAATTATTGCTCTTGGCCACCTAGTTATGAGCTGCGGAAATATAGTT
TTTGTAGACCTTATTGAACATCTGTTGTCAGAGTTGTCCAAAAATGACTCCATGTCAACAACAAGGACCT
ACATACAGTGTATTGCTGCTATTAGTAGGCAAGCTGGCCATAGAATAGGTGAATACCTTGAAAAGATAAT
TCCTTTGGTGGTAAAATTTTGTAATGTAGATGATGATGAATTAAGAGAATACTGCATTCAAGCTTTTGAA
TCATTTGTGAGAAGATGCCCTAAAGAAGTTTATCCTCATGTTTCTACCATTATAAACATTTGTCTAAAAT
ACCTTACCTATGATCCAAATTACAATTACGATGATGAAGATGAAGATGAGAATGCTATGGATGCTGACGG
TGGGGACGACGATGACCAAGGGAGTGACGATGAATATAGTGACGATGACGACATGAGTTGGAAAGTGAGA
CGCGCGGCTGCTAAGTGCCTGGACGCTGTAGTTAGCACACGGCATGAGATGCTCCCGGAGTTCTACAAGA
CTGTCTCTCCTGCGCTGATAGCCAGATTTAAAGAGCGGGAAGAAAATGTAAAGGCAGATGTTTTTCATGC
ATACCTGTCTCTTCTGAAACAAACTCGTCCAGTGCAAAGTTGGCTGTGTGACCCTGACGCAATGGAGCAG
GGAGACACGCCCCTAACAATGCTGCAGAGTCAGGTTCCCAACATTGTTAAAGCCCTGCACAAGCAGATGA
AAGAGAAGAGTGTGAAGACCCGACAGTGTTGCTTTAACATGTTAACTGAGCTGGTAAATGTGCTGCCGGG
AGCACTAACCCAGCACATTCCTGTACTGGTACCAGGAATCATTTTCTCACTGAATGATAAGTCCAGCTCA
TCAAACCTGAAGATTGATGCGCTGTCGTGTCTGTATGTCATCCTCTGTAACCACTCTCCTCAAGTTTTCC
ATCCTCATGTTCAGGCTCTGGTCCCTCCGGTGGTGGCCTGTGTGGGAGACCCGTTTTACAAGATCACTTC
AGAAGCCCTTCTTGTCACTCAGCAGCTTGTCAAAGTGATCCGTCCTCTAGACCAGCCCTCTTCGTTCGAT
GCAACGCCTTACATCAAAGATCTCTTCACCTGCACAATTAAGCGCTTAAAAGCAGCTGACATTGATCAAG
AAGTCAAGGAAAGGGCTATTTCCTGTATGGGACAGATTATTTGCAATCTTGGAGACAATTTGGGTCCTGA
TTTATCAAATACCCTACAGATTTTCTTGGAGAGACTCAAGAATGAGATTACCCGGCTAACGACCGTCAAA
GCGCTGACCCTGATTGCTGGGTCACCTTTGAAGATAGATCTGAGGCCTGTGCTGGGAGAGGGAGTCCCCA
TCCTTGCTTCATTCCTCAGGAAAAATCAGAGAGCTTTGAAACTGGGGACCCTCTCTGCCCTGGATATTCT
CATTAAGAACTACAGTGACAGCCTGACGGCCGCCATGATTGACGCAGTTCTGGATGAGCTCCCCCCCCTC
ATCAGCGAGAGCGATATGCACGTGTCGCAGATGGCTATCAGCTTCCTCACCACCCTGGCCAAGGTCTATC
CCTCCTCCCTGTCCAAGATAAGCGGATCTATCCTCAATGAACTGATTGGACTTGTGAGATCGCCTCTACT
GCAGGGAGGAGCGCTCAGTGCCATGCTAGACTTTTTCCAAGCTCTGGTTGTCACTGGAACAAACAATCTA
GGGTACATGGACTTGCTGCGCATGCTAACGGGTCCAGTTTATTCTCAGAGCACTGCTCTCACTCATAAGC
AGTCTTATTATTCCATTGCCAAATGTGTAGCTGCCCTTACTCGAGCTTGCCCTAAAGAGGGACCCGCCGT
AGTAGGTCAGTTTATTCAAGATGTCAAGAACTCAAGGTCTACAGATTCCATTCGTCTCTTAGCACTCCTT
TCTCTTGGAGAAGTTGGACACCATATTGACTTAAGTGGGCAGTTGGAATTAAAATCTGTAATATTAGAGG
CTTTCTCATCCCCTAGTGAAGAAGTCAAATCAGCTGCGTCCTATGCATTAGGCAGCATTAGTGTAGGCAA
CCTTCCTGAGTATCTGCCCTTTGTACTGCAAGAAATAACCAGCCAGCCCAAAAGGCAGTATCTTCTGCTT
CACTCTTTGAAGGAGATCATTAGCTCTGCATCGGTGGCAGGCCTTAAGCCATATGTTGAGAACATCTGGG
CCTTGCTGCTAAAGCACTGTGAGTGTGCGGAGGAAGGCACCAGGAATGTCGTGGCTGAATGTCTAGGGAA
GCTCACTCTTATTGATCCTGAAACTCTCCTCCCACGGCTTAAAGGGTATTTGATATCAGGGTCATCATAT
GCCAGGAGCTCGGTGGTTACAGCTGTGAAGTTCACGATTTCTGATCATCCTCAGCCTATTGATCCACTGT
TGAAGAACTGCATAGGTGACTTTCTAAAAACTTTGGAAGACCCAGATTTGAACGTAAGAAGAGTGGCCTT
GGTCACATTCAATTCTGCGGCCCATAACAAGCCATCACTGATACGGGACCTTCTGGACTCAGTTCTTCCA
CATCTTTACAATGAGACAAAAGTTAGGAAGGAACTTATAAGAGAGGTAGAAATGGGTCCATTTAAACACA
CGGTTGATGACGGTCTAGACATTAGAAAGGCAGCTTTTGAGTGTATGTATACACTTCTAGACAGCTGTCT
TGATAGACTGGATATCTTTGAATTTCTAAATCATGTTGAAGATGGTTTGAAGGACCATTATGATATTAAG
ATGCTAACATTTTTAATGTTGGTACGACTGTCTACCCTCTGTCCAAGTGCAGTACTACAAAGGCTGGACC
GACTTGTTGAGCCACTGCGTGCTACGTGTACAACTAAGGTAAAGGCAAACTCTGTAAAGCAGGAGTTTGA
AAAGCAAGATGAGCTAAAGCGGTCTGCCATGAGGGCAGTGGCGGCTTTGTTAACCATTCCAGAGGCAGAG
AAGAGCCCTCTTATGAGTGAATTCCAGTCACAGATCAGCTCCAACCCGGAGCTGGCAGCCATCTTTGAAA
GTATCCAGAAAGATTCTTCGTCCACCAACTTGGAATCAATGGACACGAGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216867 representing NM_027994
Red=Cloning site Green=Tags(s)

MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILRLLEDKNGEVQNLAVK
CLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTS
AIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIV
FVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFE
SFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVR
RAAAKCLDAVVSTRHEMLPEFYKTVSPALIARFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQ
GDTPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSS
SNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFD
ATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGPDLSNTLQIFLERLKNEITRLTTVK
ALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPL
ISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNL
GYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALL
SLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLL
HSLKEIISSASVAGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSY
ARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDSVLP
HLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIK
MLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAE
KSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027994
ORF Size 3690 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027994.1, NP_082270.1
RefSeq Size 8209 bp
RefSeq ORF 3693 bp
Locus ID 71902
UniProt ID Q6ZQ38
Cytogenetics 10 67.08 cM
Summary Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027994" in other vectors (4)
SKU Description Size Price
MC224016 Cand1 (untagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1), (10ug) 10 ug
$1,179.00
MR216867 Cand1 (Myc-DDK-tagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1) 10 ug
$1,606.00
MR216867L3 Lenti ORF clone of Cand1 (Myc-DDK-tagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1) 10 ug
$1,906.00
MR216867L4 Lenti ORF clone of Cand1 (mGFP-tagged) - Mouse cullin associated and neddylation disassociated 1 (Cand1) 10 ug
$1,906.00

file-alt Citations

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