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Wdr4 (NM_021322) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216848
Wdr4 (tGFP-tagged) - Mouse WD repeat domain 4 (Wdr4), (10ug)
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ORF Plasmid:
tGFP DDK
  $932.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wdr4
Synonyms AI415180; AI448349; D530049K22Rik; Wh
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216848 representing NM_021322
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGACTACGCCCTGCGCGCATGCTCCTGGACGGCACGCCGTTCGCGCGGCGCCGAGTGACATCACTGA
CAAGCGCCAACAGGAAGGGCGCAGCGCGTCGCACGTGTCCGGAGGCGGCGGGCGGGCCCATGGCGAGCTC
TGCGGGGCTGGCACTGTGCGCCCAGACGCTGGTGGTGCGAGGAGGCAGCCGGTTCCTAGCCTTCTCCACT
ACGGGCAGTGATGATGACTGTGTCTTCACATACGACTGCAGTACTGCAGAGAAGAAGGCCACGCCAGAAG
ATAAAGGGGAGGACGGACAGCCCGCAGACACAGGGAGTGACTCGATTCTGGCGTCCACCTTCTCCAAGTC
TGGCCGCTATTTTGCTTTAACAGATGACAGTAAGCGTCTGATTCTTTTCCGTACAAAACCATGGCAATGT
CTGAGTGTCAGGATGGTGGTGCGGAGGTGCACCGCCCTGACCTTCACAGCCTCAGAGGACCGAGTCTTGG
TGGCTGACAAGTCTGGAGACGTCTACTCCTTTTCGGTGCTGGAGCCAGATGGATGTGGCAGGCTGGAGCT
TGGGCACCTCTCCATGCTGCTAGACGTGGCTGTGAGTCCTGATGACCAGTTTGTGCTTACTGCAGACCGG
GATGAGAAGATCCGGGTCAGCTGGGCTGCTGCCCCGCATAGCATCGAGTCTTTCTGCCTGGGACACACTG
AGTTTGTGAGCCGCATCCTTGTAGTGCCCAGTCATCCTGAACTGCTGCTTTCTTCCTCTGGGGATGGCAC
CCTGAGACTCTGGGAGTACAGAAGCGGTAGGCAGCTGCAGTGCTGTGACCTGGCCGGCCTACAGGAGCCT
GGAGAGCAGCCAGGCCACAAGGGGTTGGCCGCGTCCAGGATTGCATTCTGGGGACAGGAGAGCTATGTGG
TGCTTCTGTGTGAGTGCGTTCCCGTGGTCTTCGTCTTCCAGCTTGATGCCAGCAGACAGCAGCTGGTGTT
CAGACAGCGGCTGACTTTCCCTCACCGAGTGTGGGATGTTGTGTTTGAGGAGGCCCGGGGGCTGTGGGTT
CTACAGGACTGCCGTGATGCCCCCCTGGTGCTCTGGAGGCCTGTGGGTGGTGAGTGGCAGGCTGCTCCAG
ACGGTGCTGTGTCCCCGAGACTCTGCAGCCATCTCCGTGAGAGCTGGGCCATGCTGGAAGGTTCTGTTGG
TACAGATGACAGCTTCCGCAGCCTGTACAAGGCCACCTTTGACAACATGACCTCTTACCTGAAGAAAAAG
GAAGAGAGACTGCAGCAGCAGCTGAAGAAGAAGCGGCAAAGGAGCCCCTTCCCAGGGTCCCCGGAACAGA
CCAAAAAGGCGTGCCCGGGCCAGTCAGCCCTTAGTTGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216848 representing NM_021322
Red=Cloning site Green=Tags(s)

MRLRPARMLLDGTPFARRRVTSLTSANRKGAARRTCPEAAGGPMASSAGLALCAQTLVVRGGSRFLAFST
TGSDDDCVFTYDCSTAEKKATPEDKGEDGQPADTGSDSILASTFSKSGRYFALTDDSKRLILFRTKPWQC
LSVRMVVRRCTALTFTASEDRVLVADKSGDVYSFSVLEPDGCGRLELGHLSMLLDVAVSPDDQFVLTADR
DEKIRVSWAAAPHSIESFCLGHTEFVSRILVVPSHPELLLSSSGDGTLRLWEYRSGRQLQCCDLAGLQEP
GEQPGHKGLAASRIAFWGQESYVVLLCECVPVVFVFQLDASRQQLVFRQRLTFPHRVWDVVFEEARGLWV
LQDCRDAPLVLWRPVGGEWQAAPDGAVSPRLCSHLRESWAMLEGSVGTDDSFRSLYKATFDNMTSYLKKK
EERLQQQLKKKRQRSPFPGSPEQTKKACPGQSALSC

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021322
ORF Size 1368 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021322.2, NP_067297.2
RefSeq Size 3906 bp
RefSeq ORF 1371 bp
Locus ID 57773
UniProt ID Q9EP82
Cytogenetics 17 B1
Summary Non-catalytic component of a methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:29983320). In the methyltransferase complex, it is required to stabilize and induce conformational changes of the catalytic subunit (By similarity). Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA (PubMed:29983320). Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs (By similarity). Also required for methylation of a specific subset of miRNAs, such as let-7 (By similarity). Acts as a regulator of embryonic stem cell self-renewal and differentiation (PubMed:29983320). Independently of METTL1, also plays a role in genome stability: localizes at the DNA replication site and regulates endonucleolytic activities of FEN1 (PubMed:29574139).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_021322" in other vectors (4)
SKU Description Size Price
MC201825 Wdr4 (untagged) - Mouse WD repeat domain 4 (Wdr4), (10ug) 10 ug
$457.00
MR216848 Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (Wdr4) 10 ug
$732.00
MR216848L3 Lenti ORF clone of Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (Wdr4) 10 ug
$1,032.00
MR216848L4 Lenti ORF clone of Wdr4 (mGFP-tagged) - Mouse WD repeat domain 4 (Wdr4) 10 ug
$1,032.00

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