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Gucy2g (NM_001081076) Mouse Tagged ORF Clone

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SKU
MG216833
Gucy2g (tGFP-tagged) - Mouse guanylate cyclase 2g (Gucy2g), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,157.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Gucy2g or NM_001081076"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gucy2g
Synonyms 2410077I05Rik; GC-G
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216833 representing NM_001081076
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCTAGGACACGCTCTGAGTCTCCACTAGAACCTAGGCTCTATGCTGGGGCAGGAAGCCGTGCAG
ATCATCCATCTCTGGTTTTGATGCTGTCTGTCGTTATGCTAGTGACTTGTCTCGAAGCTGCTAAGCTCAC
CGTGGGGTTCCATGCCCCCTGGAACATCTCTCATCCATTTAGTGTTCAAAGGTTGGGGGCAGGACTCCAG
ACTGTTGTGGACAAGCTCAACTCAGAGCCAGTGGGTCTTGGGAACGTGAGCTGGGAGTTCACTTACACCA
ATTCAACATGCAGTGCCAAGGAGTCCCTTGCGGTTTTCATCGACCAGGTCCAGAAAGAACACATTTCTGC
TCTGTTTGGACCAGCATGCCCAGAAGCAGCAGAGGTTATTGGTTTATTGGCCTCTGAGTGGAACATCCCT
TTGTTTGACTTCGTTGGACAAATGGCAGCCCTGAAAGACCACTTCTGGTGTGACACCTGCGTGACACTTG
TGCCACCCAAGCAGGAAATCAGTGCGGTGCTTCGGGAAAGCCTGCGGTACCTGGGCTGGGAACATATCGG
GGTGTTTGGAGGTTCCTCTGCAGATTCCTCCTGGGAACAAGTGGATGAGATGTGGGGGGCTGTTGAGGAT
GGACTCCAGTTCCATTTCAACATCACTGCCAGCATGAGGTACAACAGCAGCAGCTCAGACCTTCTGCAGG
AAGGCCTCAGGAACATGTCGTATGTCGCCAGGGTCATCATCCTAATCTGCAGCTCGGAGGATGCAAGGCG
CATTCTGCAGGCTGCAGTGGACCTGGGACTCGACACTGGAGAATTCGTTTTCATCCTCTTGCAGCAGCTG
GAGGACAGTTTTTGGAAGGAAGTACTGACAAAGGACAAGGTGATACGCTTTCCCAAAGTCTATGAGTCGG
TGTTTCTCATCGCACCCAGCGCCTATGGAGGAGGCATTGGAGATGACGGTTTCCGGAAGCAAGTCTCCCA
AGAACTGAGGAGGCCACCCTTTCAAAGCTCCATCACCTCAGAGGACCAGGTGAGCCCGTACTCTGCCTAC
CTGCACGATGCTCTCTTACTCTACGCTCAGACTGTGGAGGAAATGAGGAAGGCTGAGAAAGACTTCCGGG
ATGGGCGGCAGCTGATTAGCACCCTGAGAGCTGGTCAGGTCACACTGCAGGGAATCACCGGTCCTGTGCT
TTTGGACTCCCAGGGGAAAAGGCACGTGGATTACTCCGTCTATGCTCTGCAGGAATCTGGAAACAGGTCC
CTCTTCCTTCCCTTTCTTCATTATGACAGTTTTCAGAAAGTAATCAGACCCTGGAGGAATGACTCGAACA
CCTCATGGCCCCATGGCTCCCTTCCTGAGTATAAACCTGGCTGTGGATTTCATAACGACCTTTGCAAAAC
GAAGCCACCCACTGTAGCAGGTATGACAGTGACTGTGACTGCTGTGATCCCCACGGTGACGTTCCTGGTT
CTTGCCTCGGCAGCTGCCATCACAGGTCTGATGTTATGGAGGTTGAGAGGGAAAGTGCAGAGCCATCCTG
GAGACACCTGGTGGCAGATCCGTTATGACAGCATCACTCTTCTGCCTCAGCACAAACTGTCCCACCGAGG
CACACCTGTGTCAAGACGTAATGTTAGCGATACATCTACTGTGAAGGCTTCTGCAGATTGTGGCTCCTTG
GTCAAGAGGCACCAGGATGAAGAGCTGTTCTTTGCCCCCGTGGGGCTTTACCAGGGAAATCAGGTGGCCC
TCTGCTATATTGGTGACGAAGCAGAAGCTTGGGTTAAGAAGCCAACTGTGCGGCGGGAAGTCTGCCTGAT
GTGCGAACTAAAGCATGAAAACATCGTCCCCTTCTTTGGTGTTTGCACAGAGCCACCTAACATCTGCATC
GTTACACAGTACTGCAAAAAAGGAAGTCTCCAGGATGTTATGAGGAACTCCGATCATGAGATAGATTGGA
TATTCAAGCTCTCCTTTGCGTATGACATAGTCAATGGCTTGCTGTTCCTGCATGGGAGCCCACTGAGGTC
TCACGGCAACCTGAAACCTTCCAACTGCCTGGTAGATAGTCACATGCAGCTGAAGCTGTCAGGATTTGGG
TTGTGGGAGTTCAAGCACGGCAGCACATGGAGGAGCTACAACCAGGAGGCCACAGACCATTCAGAGCTCT
ACTGGACAGCCCCAGAGCTGCTGCGGCTTCGGGAGAGTCCCTGTTCTGGCACCCCTCAGGGAGACGTTTA
CAGCTTTGCCATTCTCCTGAGGGACTTGATCCACCAGCAGGCCCACGGGCCCTTTGAGGACCTCGAGGCA
GCTCCAGAGGAAATCATCAGCCGCATCAAAGACCCCCGGGCACCCGTTCCGCTTCGGCCGTCCCTGTTGG
AGGATAAAGGTGATGGAAGGATTGTGGCCCTAGTGAGGGAGTGCTGGGATGAATCTCCAGAGCTAAGGCC
CATCTTCCCTTCCATCAAGAAGACGTTACGAGAAGCCAGTCCCAGAGGCCACGTGAGCATCCTGGATAGC
ATGATGGGGAAGCTGGAAACGTACGCCAATCACCTGGAGGAAGTGGTAGAGGAGCGGACCCGCGAGTTGG
TGGCAGAGAAAAGAAAGGTGGAGAAACTCCTGTCTACCATGTTGCCAAGCTTCGTGGGAGAACAGCTAAT
AGCCGGAAAGTCTGTAGAACCGGAACACTTTGAGTCTGTCACCATCTTCTTCTCTGACATCGTGGGGTTC
ACAAAGCTGTGTTCCCTCAGCTCCCCCCTGCAAGTGGTGAAGCTCCTCAACGACCTGTACAGCTTGTTTG
ACCACACGATACAGAGCCATGATGTATACAAGGTTGAAACCATTGGAGATGCCTACATGGTGGCAAGTGG
GCTCCCCATCCGCAACGGAGCTCAGCATGCCGACGAGATTGCCACCATGGCCCTGCACTTACTCAGTGTC
ACCACCCACTTCCAGATCGGGCACATGCCTGAGGAGAGGCTCAAGCTCCGCATAGGCCTCCACACAGGTC
CTGTGGTGGCCGGTGTGGTGGGAATCACCATGCCCAGGTACTGTCTGTTTGGAGACACTGTGAACATGGC
ATCCAGGATGGAAAGCAGCAGCTTGCCTCTCCGGATTCACGTTTCCCAGAGCACCGCAGGAGCTCTTCTA
GCAGCAGGGGGGTACCATCTGCAAAAGAGAGGCACCATTTCAGTCAAGGGCAAGGGAGAACAGACGACCT
TCTGGCTGAAAGGCAAAGATGGCTTCCCTGTCCCACTCCCTGAGTTTACGGAGGAAGAAGCTAAAGTTTC
AGAAATATTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216833 representing NM_001081076
Red=Cloning site Green=Tags(s)

MASRTRSESPLEPRLYAGAGSRADHPSLVLMLSVVMLVTCLEAAKLTVGFHAPWNISHPFSVQRLGAGLQ
TVVDKLNSEPVGLGNVSWEFTYTNSTCSAKESLAVFIDQVQKEHISALFGPACPEAAEVIGLLASEWNIP
LFDFVGQMAALKDHFWCDTCVTLVPPKQEISAVLRESLRYLGWEHIGVFGGSSADSSWEQVDEMWGAVED
GLQFHFNITASMRYNSSSSDLLQEGLRNMSYVARVIILICSSEDARRILQAAVDLGLDTGEFVFILLQQL
EDSFWKEVLTKDKVIRFPKVYESVFLIAPSAYGGGIGDDGFRKQVSQELRRPPFQSSITSEDQVSPYSAY
LHDALLLYAQTVEEMRKAEKDFRDGRQLISTLRAGQVTLQGITGPVLLDSQGKRHVDYSVYALQESGNRS
LFLPFLHYDSFQKVIRPWRNDSNTSWPHGSLPEYKPGCGFHNDLCKTKPPTVAGMTVTVTAVIPTVTFLV
LASAAAITGLMLWRLRGKVQSHPGDTWWQIRYDSITLLPQHKLSHRGTPVSRRNVSDTSTVKASADCGSL
VKRHQDEELFFAPVGLYQGNQVALCYIGDEAEAWVKKPTVRREVCLMCELKHENIVPFFGVCTEPPNICI
VTQYCKKGSLQDVMRNSDHEIDWIFKLSFAYDIVNGLLFLHGSPLRSHGNLKPSNCLVDSHMQLKLSGFG
LWEFKHGSTWRSYNQEATDHSELYWTAPELLRLRESPCSGTPQGDVYSFAILLRDLIHQQAHGPFEDLEA
APEEIISRIKDPRAPVPLRPSLLEDKGDGRIVALVRECWDESPELRPIFPSIKKTLREASPRGHVSILDS
MMGKLETYANHLEEVVEERTRELVAEKRKVEKLLSTMLPSFVGEQLIAGKSVEPEHFESVTIFFSDIVGF
TKLCSLSSPLQVVKLLNDLYSLFDHTIQSHDVYKVETIGDAYMVASGLPIRNGAQHADEIATMALHLLSV
TTHFQIGHMPEERLKLRIGLHTGPVVAGVVGITMPRYCLFGDTVNMASRMESSSLPLRIHVSQSTAGALL
AAGGYHLQKRGTISVKGKGEQTTFWLKGKDGFPVPLPEFTEEEAKVSEIL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081076
ORF Size 3300 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081076.3
RefSeq Size 3886 bp
RefSeq ORF 3303 bp
Locus ID 73707
UniProt ID Q6TL19
Cytogenetics 19 51.18 cM
Summary Probably plays a specific functional role in the testis by binding to yet not identified ligand.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081076" in other vectors (4)
SKU Description Size Price
MC223561 Gucy2g (untagged) - Mouse guanylate cyclase 2g (Gucy2g), (10ug) 10 ug
$1,054.00
MR216833 Gucy2g (Myc-DDK-tagged) - Mouse guanylate cyclase 2g (Gucy2g) 10 ug
$589.00 $957.00
MR216833L3 Lenti ORF clone of Gucy2g (Myc-DDK-tagged) - Mouse guanylate cyclase 2g (Gucy2g) 10 ug
$1,257.00
MR216833L4 Lenti ORF clone of Gucy2g (mGFP-tagged) - Mouse guanylate cyclase 2g (Gucy2g) 10 ug
$1,257.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.