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Ces1c (NM_007954) Mouse Tagged ORF Clone

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SKU
MG216728
Ces1c (tGFP-tagged) - Mouse esterase 1 (Es1), (10ug)
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ORF Plasmid:
tGFP DDK
  $973.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ces1c
Synonyms Ces-N; Ee-1; Ee1; Es-4; Es-N; Es1; Es4; EsN; PESN
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216728 representing NM_007954
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCTCCATGCTCTGGTCTGGGCTTCTCTTGCTGTTTGCCCAATTCTGGGACACTCACTCTTACCAC
CTGTGGTGGACACCACACAAGGCAAAGTCCTGGGGAAGTATATCAGCTTAGAAGGATTTGAACAGCCTGT
GGCCGTCTTCCTGGGAGTCCCCTTTGCCAAGCCCCCTCTTGGATCTCTGAGGTTTGCTCCACCACAGCCT
GCAGAGCCCTGGAGCTTCGTGAAGAATGCCACCTCCTACCCTCCTATGTGCTCCCAAGATGCAGGTTGGG
CAAAGATACTCTCAGATATGTTCAGCACCGAAAAGGAGATCCTGCCTCTCAAGATTTCGGAAGACTGTCT
CTACCTGAATATTTACAGTCCTGCCGATTTGACAAAAAGCAGCCAATTGCCTGTGATGGTGTGGATCCAT
GGAGGTGGACTGGTGATAGGCGGAGCATCACCCTATAATGGACTAGCTCTCTCTGCCCATGAGAACGTGG
TGGTAGTAACCATTCAATACCGCCTGGGTATTTGGGGATTATTTAGCACAGGTGATGAACACAGCCCAGG
AAACTGGGCTCACCTGGACCAACTGGCTGCACTACGCTGGGTCCAAGATAACATTGCAAACTTTGGAGGG
AACCCGGATTCCGTGACCATCTTTGGAGAGTCATCAGGGGGTATCAGTGTCTCTGTCCTTGTCTTATCTC
CTCTGGGCAAGGACCTCTTCCACAGAGCCATTTCTGAGAGTGGTGTGGTCATAAATACAAACGTGGGCAA
GAAGAATATACAGGCTGTGAATGAAATAATAGCTACTCTTTCCCAGTGTAATGACACCTCATCAGCTGCC
ATGGTTCAGTGCTTGCGCCAGAAGACAGAGAGTGAGCTCTTGGAGATTTCAGGGAAACTGGTACAGTACA
ATATCTCCCTGTCCACCATGATTGACGGAGTGGTGCTGCCAAAGGCACCAGAAGAGATCCTGGCTGAGAA
GAGTTTCAACACTGTCCCCTACATAGTGGGCTTCAACAAGCAAGAGTTTGGCTGGATAATTCCAATGATG
TTGCAAAATCTACTCCCTGAAGGCAAAATGAATGAGGAAACGGCCAGTCTTCTCTTGAGGAGGTTCCACT
CTGAACTTAACATCTCTGAAAGTATGATTCCAGCAGTCATTGAGCAGTACTTAAGAGGGGTAGATGACCC
TGCCAAAAAGAGTGAGCTTATCCTGGACATGTTTGGAGATATATTTTTTGGTATCCCGGCTGTGCTCTTG
TCCCGTAGCCTCAGAGATGCTGGAGTGTCCACCTACATGTATGAGTTTCGGTATCGCCCAAGCTTCGTGT
CTGACAAGAGACCCCAGACGGTTGAGGGTGATCATGGCGATGAAATCTTCTTTGTATTCGGGGCTCCACT
ATTAAAAGAGGGTGCCTCAGAAGAGGAAACCAACCTCAGCAAGATGGTGATGAAATTCTGGGCCAACTTT
GCTCGAAATGGGAACCCTAATGGGGAGGGGCTGCCTCACTGGCCAGAATATGATGAGCAGGAAGGGTATC
TACAGATTGGAGCCACTACCCAGCAAGCCCAGAGACTGAAAGCAGAGGAAGTGGCTTTCTGGACTGAGCT
CCTGGCTAAGAATCCACCTGAGACAGACCCTACTGAACACACAGAACACAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216728 representing NM_007954
Red=Cloning site Green=Tags(s)

MWLHALVWASLAVCPILGHSLLPPVVDTTQGKVLGKYISLEGFEQPVAVFLGVPFAKPPLGSLRFAPPQP
AEPWSFVKNATSYPPMCSQDAGWAKILSDMFSTEKEILPLKISEDCLYLNIYSPADLTKSSQLPVMVWIH
GGGLVIGGASPYNGLALSAHENVVVVTIQYRLGIWGLFSTGDEHSPGNWAHLDQLAALRWVQDNIANFGG
NPDSVTIFGESSGGISVSVLVLSPLGKDLFHRAISESGVVINTNVGKKNIQAVNEIIATLSQCNDTSSAA
MVQCLRQKTESELLEISGKLVQYNISLSTMIDGVVLPKAPEEILAEKSFNTVPYIVGFNKQEFGWIIPMM
LQNLLPEGKMNEETASLLLRRFHSELNISESMIPAVIEQYLRGVDDPAKKSELILDMFGDIFFGIPAVLL
SRSLRDAGVSTYMYEFRYRPSFVSDKRPQTVEGDHGDEIFFVFGAPLLKEGASEEETNLSKMVMKFWANF
ARNGNPNGEGLPHWPEYDEQEGYLQIGATTQQAQRLKAEEVAFWTELLAKNPPETDPTEHTEHK

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007954
ORF Size 1662 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007954.4, NP_031980.2
RefSeq Size 1850 bp
RefSeq ORF 1665 bp
Locus ID 13884
UniProt ID P23953
Cytogenetics 8 44.99 cM
Summary Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Involved in the extracellular metabolism of lung surfactant.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007954" in other vectors (4)
SKU Description Size Price
MC218942 Ces1c (untagged) - Mouse carboxylesterase 1C (Ces1c), (10ug) 10 ug
$826.00
MR216728 Ces1c (Myc-DDK-tagged) - Mouse carboxylesterase 1C (Ces1c) 10 ug
$773.00
MR216728L3 Lenti ORF clone of Ces1c (Myc-DDK-tagged) - Mouse carboxylesterase 1C (Ces1c) 10 ug
$1,073.00
MR216728L4 Lenti ORF clone of Ces1c (mGFP-tagged) - Mouse carboxylesterase 1C (Ces1c) 10 ug
$1,073.00

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