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Cd101 (NM_001167906) Mouse Tagged ORF Clone

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SKU
MG216655
Cd101 (tGFP-tagged) - Mouse CD101 antigen (Cd101) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,549.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cd101
Synonyms EWI-101; Gm734; Gm1016; Igsf2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216655 representing NM_001167906
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATGCATCCTGTGTGTGGCATCTCTCTTTCTCTCTCTAACTAAGTTCAGCATCGGCCAGAGGGAAG
TAAAAATTCAAGAAGGCCCTCTGTACAGAGCCGAAGGTTACCCTGTCAGCATCAGGTGCACCGTAAGTGG
TCATCAGGGTCCTTCCACGCAGGATTTCCGGTGGTCTATTTACCTGCCAAGCGCCCCGACCAAGGAAGTC
CAGATCATCAGTACCAAGGATGCCGGCTTCTCTTACGCAGTGTATGCCCAGAGGGTGCAAAGCAAGGAGA
TCTACATAGAGAGGCTGCAGGGTGACTCGGTCCTGCTGCATATCTCAAAACTCCAGATGAAAGATGCTGG
CGAGTACGAGTGCCACACACCCAACACGGATGGGAAGTACTTTGGAAGTTACAGTGCAAAGACAAATCTT
ACTGTGGTTCCCGACACCCTGTCTGCCACCATGCCCTCCCAGACGCTCAGTAAGAAGGAAGGTGAGCCCT
TGGAACTCACCTGTGAGACAACCAAAGCCACCGTGCAACACACCCATCTCTCTCTCACCTGGTACCTGAT
GCAGGAAGGAGGAGGGAGCCAAGCCACTGAGATCGTTTCTCTCTCCAAGGACTTTGTATTGACCCCTGGG
TCCTCCTATGCAGACAGGTTTGTGGCCGGTGATGTACGGCTGGACAAGCTTGGAGCCACTTCCTTCAGGC
TGTCTGTAGGCAAGCTCCAGCCCTCAGATCAGGGCCAGGTGTTCTGTGAGGCCACAGAATGGATTCAGGA
TCCAGATGAAACGTGGACTTTGATCACAAGAAAGCAGACAGATCAAACAGCTCTGAGGATCCAGCCGGCA
GCAAGAGATTTTACAGTGAGCATCACAGCCAGTAGCTCACCTGATGAAGGAAAACCCTTGGAACTGGTTT
GCCTGGCTGTTGGCAGAGATGGTAACCCGCAGCTTCAGGGTGTGTGGTTTCTCAATGGGAAGGAAATTGC
CCAGACTGATGCTGGTGGGGTCCTGGACCTGAAGAGAGACTACAGAGACAGAGCGAGCCAAGGCCAGCTG
CAGGTGTCAAAGTTAAGTGCCCAGACGTTCTCTCTCAAGATCTTCTCCGTGGGTCCAGAGGATGTAGGCA
CCTACAGTTGTGAAGTGGCAGAGGTGGCGAGGACTCAGATGGGCTCCTGGCAGGTACTTCAGAGAAAGCA
GTCACCAGGCTACCGGGTGCAGCTGAGGGAGCCAGCAGCAAGAAGTGTGACCGTGTCTGCCGAGCAGCGT
ACTGTGTGGGAAGGAGAGACGCTTACCCTTCTCTGCAAGGCAGCTGGGGATGTGAGTGCTCTATCTGTGA
GTTGGTGGCTCACCCCACAGGACCAGTCCACACCCGTGTTTGTGGCTGGCATGGGGCAAGATGGTACTGT
GCAGCTGGGAGTCTCTTCTCCGGGCCCTGCGCACCGTGGTAACAGGAGGCTGGAGAAAGTGGACTGGGCC
ACCTTTCGGCTGGAGATTGCCTCTGCCATGGTCACAGACAGCGGTACCTATGAATGCAGGGTGTCAGAGA
GACTCCAGAACCAGGCCAAAGGTTTGCAGTCAACCCAGAAGATTTCAGTCACTGTAAAATCTCTGAAGTC
AAGTTTACGAGTTAATCTGATGAGCCGTCAGCCACAAGTGATGCTAGCCCATACCTTCCACCTGTCCTGT
GTAGTGAGGGCCAACTACTCGGATCTCAAGCTGCCGTTCTCGGTAACGTGGCAGTTCCAGCCAGCGGGCT
CTGGAGCCTTTCATCGGCTTATTCGAATTGCCCACAATGGCACCGTTGAATGGGGGGACGTCCTCTCCCA
GATCCACAGGAAGACGAAGGTGTCACAGTCTTTCTTTCGTTCTCAACTCCAAATCTACGATGCAGCTATG
GAGGAGACAGGGGTGTATCGGTGTACAGTGGAGGTTTATGACAGAGATTCCATATGCACAAGTGGCCCAG
CCAGAGTATCTGCCACCTCTAACCTATTAATGATTACTGTCACCTTTCCAGAGAGCAAACTGAGCGTGAA
CTCAAGCAGTCAGGTCCAAGAGCTATCCATCAGCTCCAGCACTCAGATAGAATGTGCCATCTTGTCCCGA
TCTGCTGGAAACCTTCCGTTATCCATCATTTGGTACTTCTCTTCCGTTTCTGCAAATGCATCCTATCTGA
AAATCCTAGAAATGGACCAAAGCAGTGTTGTAAAATATGGGGACGAATTTCAAACCCCTCGGAGCAAGCA
AAAATTTTATTCTGAGAAAGTTTCTCAAGACTTATTTCTGCTGAACATTCTGAGTGTGGAGGACAGTGAC
CAGGGCCACTACCACTGTGCTGTAGAGGAATGGCTCTTATCTACAAATGACACTTGGCAAAAGCTTGAAA
GAAAGACTTCAGGACTCACAGAATTGAAACTCAGGCCCACAGGAAGCCAGGTCCATGTCTCCAAAGTGAA
TTGGACAGGAAACGCTACCGAGTATGGAGAGGCGGGCTTCAGCTGCAGCCTAGATGGCTCAGGCAGCACA
GCTTCCCTATACTCTGTGACATGGTACCGGGGCAGAGGAACTGCCACGGCTACTGCTGCCGCAGTTGCCA
ATGCCACTGCCACCATCACTGCCCCCGCAGGGAGTCAAATGCTGGTGCACCTGCAGTATGACGGCTTGCT
GCAGTATGGCAGAGAGGGGAGCAGGAGGCTCCAGCACTGCTACCGATCCTCTCCCACAGACTTCGTCCTG
AAGCTGCATCGGGTGGAAATGGAGGATGCTGGGATATATTGGTGCAGGGTGACTGAGTGGCAGCAACATG
GCCACCCAGGCAAGTGGATCAACCAAGCCTCAGGCGAGTCGCAACGAATGGTGCTCAGGGTGCTGCGCTC
AGAGCCCACGGTCTCCTCCCTGATCTGCTCCTCGGGGCCCTTGCTCCACTTCCTCATCGTCTGCCCCTTC
GTCATGCTGCTCCTTCTGGCCACGTCCTTCCTCTGCTTGTACCGGAAGGCCAGGAAGTTGTCACAGCTGA
GTCTCAGTGCAAAGAAAGAAAAGGCTCTCTGGGTGGGCATGAGAAAGACATCACTTCAGAAGGAAGCTGG
AGAGGAGAGTGGACACTAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216655 representing NM_001167906
Red=Cloning site Green=Tags(s)

MACILCVASLFLSLTKFSIGQREVKIQEGPLYRAEGYPVSIRCTVSGHQGPSTQDFRWSIYLPSAPTKEV
QIISTKDAGFSYAVYAQRVQSKEIYIERLQGDSVLLHISKLQMKDAGEYECHTPNTDGKYFGSYSAKTNL
TVVPDTLSATMPSQTLSKKEGEPLELTCETTKATVQHTHLSLTWYLMQEGGGSQATEIVSLSKDFVLTPG
SSYADRFVAGDVRLDKLGATSFRLSVGKLQPSDQGQVFCEATEWIQDPDETWTLITRKQTDQTALRIQPA
ARDFTVSITASSSPDEGKPLELVCLAVGRDGNPQLQGVWFLNGKEIAQTDAGGVLDLKRDYRDRASQGQL
QVSKLSAQTFSLKIFSVGPEDVGTYSCEVAEVARTQMGSWQVLQRKQSPGYRVQLREPAARSVTVSAEQR
TVWEGETLTLLCKAAGDVSALSVSWWLTPQDQSTPVFVAGMGQDGTVQLGVSSPGPAHRGNRRLEKVDWA
TFRLEIASAMVTDSGTYECRVSERLQNQAKGLQSTQKISVTVKSLKSSLRVNLMSRQPQVMLAHTFHLSC
VVRANYSDLKLPFSVTWQFQPAGSGAFHRLIRIAHNGTVEWGDVLSQIHRKTKVSQSFFRSQLQIYDAAM
EETGVYRCTVEVYDRDSICTSGPARVSATSNLLMITVTFPESKLSVNSSSQVQELSISSSTQIECAILSR
SAGNLPLSIIWYFSSVSANASYLKILEMDQSSVVKYGDEFQTPRSKQKFYSEKVSQDLFLLNILSVEDSD
QGHYHCAVEEWLLSTNDTWQKLERKTSGLTELKLRPTGSQVHVSKVNWTGNATEYGEAGFSCSLDGSGST
ASLYSVTWYRGRGTATATAAAVANATATITAPAGSQMLVHLQYDGLLQYGREGSRRLQHCYRSSPTDFVL
KLHRVEMEDAGIYWCRVTEWQQHGHPGKWINQASGESQRMVLRVLRSEPTVSSLICSSGPLLHFLIVCPF
VMLLLLATSFLCLYRKARKLSQLSLSAKKEKALWVGMRKTSLQKEAGEESGHY

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001167906
ORF Size 3099 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001167906.2
RefSeq Size 3254 bp
RefSeq ORF 3102 bp
Locus ID 630146
UniProt ID A8E0Y8
Cytogenetics 3 F2.2
Summary Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001167906" in other vectors (4)
SKU Description Size Price
MC223302 Cd101 (untagged) - Mouse CD101 antigen (Cd101), transcript variant 1, (10ug) 10 ug
$1,440.00
MR216655 Cd101 (Myc-DDK-tagged) - Mouse CD101 antigen (Cd101), transcript variant 1 10 ug
$1,349.00
MR216655L3 Lenti ORF clone of Cd101 (Myc-DDK-tagged) - Mouse CD101 antigen (Cd101), transcript variant 1 10 ug
$1,649.00
MR216655L4 Lenti ORF clone of Cd101 (mGFP-tagged) - Mouse CD101 antigen (Cd101), transcript variant 1 10 ug
$1,649.00

file-alt Citations

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