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F2rl2 (NM_010170) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216610
F2rl2 (tGFP-tagged) - Mouse coagulation factor II (thrombin) receptor-like 2 (F2rl2), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol F2rl2
Synonyms F730031A08; PAR3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216610 representing NM_010170
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAATCCTTATCTTGGTTGCAGCTGGGCTGCTGTTTCTGCCAGTCACTGTTTGCCAAAGTGGCATAA
ATGTTTCAGACAACTCAGCAAAGCCAACCTTAACTATTAAGAGTTTTAATGGGGGTCCCCAAAATACCTT
TGAAGAATTCCCACTTTCTGACATAGAGGGCTGGACAGGAGCCACCACAACTATAAAAGCGGAGTGTCCC
GAGGACAGTATTTCAACTCTCCACGTGAATAATGCTACCATAGGATACCTGAGAAGTTCCTTAAGTACCC
AAGTGATACCTGCCATCTACATCCTGCTGTTTGTGGTTGGTGTACCAGCCAACATCGTGACCCTGTGGAA
ACTCTCCTTAAGGACCAAATCCATCAGTCTGGTCATCTTTCACACCAACCTGGCCATCGCAGATCTCCTT
TTCTGTGTCACACTGCCATTTAAGATCGCCTACCATCTCAATGGCAACAACTGGGTATTTGGCGAGGTCA
CGTGCCGGATCACCACGGTTGTTTTCTACGGCAACATGTACTGCGCTATCCTGATCCTCACCTGCATGGG
CATCAACCGCTACCTGGCCACGGCTCACCCTTTCACATACCAGAAGCTGCCCAAACGCAGCTTCTCCATG
CTCATGTGTGGCATGGTGTGGGTCATGGTTTTCTTATACATGCTGCCCTTTGTCATCCTGAAGCAGGAGT
ACCACCTCGTCCACTCCGAGATCACCACCTGCCACGATGTCGTTGACGCGTGCGAGTCCCCATCATCCTT
CCGATTCTACTACTTCGTCTCCTTAGCATTCTTTGGGTTCCTCATCCCGTTTGTGATCATCATCTTCTGT
TACACGACTCTCATCCACAAACTTAAATCAAAGGATCGGATATGGCTGGGCTACATCAAGGCCGTCCTCC
TCATCCTTGTGATTTTCACAATTTGCTTTGCCCCCACCAACATCATACTCGTAATCCACCATGCCAACTA
CTACTACCACAATACCGACAGCTTGTACTTTATGTATCTTATTGCTCTGTGCCTGGGGAGCCTGAATAGC
TGCCTAGATCCATTCCTTTACTTTGTCATGTCGAAAGTTGTAGATCAGCTTAATCCT

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216610 representing NM_010170
Red=Cloning site Green=Tags(s)

MKILILVAAGLLFLPVTVCQSGINVSDNSAKPTLTIKSFNGGPQNTFEEFPLSDIEGWTGATTTIKAECP
EDSISTLHVNNATIGYLRSSLSTQVIPAIYILLFVVGVPANIVTLWKLSLRTKSISLVIFHTNLAIADLL
FCVTLPFKIAYHLNGNNWVFGEVTCRITTVVFYGNMYCAILILTCMGINRYLATAHPFTYQKLPKRSFSM
LMCGMVWVMVFLYMLPFVILKQEYHLVHSEITTCHDVVDACESPSSFRFYYFVSLAFFGFLIPFVIIIFC
YTTLIHKLKSKDRIWLGYIKAVLLILVIFTICFAPTNIILVIHHANYYYHNTDSLYFMYLIALCLGSLNS
CLDPFLYFVMSKVVDQLNP

SGPTRTRRLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_010170
ORF Size 1107 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010170.4, NP_034300.3
RefSeq Size 2464 bp
RefSeq ORF 1110 bp
Locus ID 14064
UniProt ID O08675
Cytogenetics 13 50.38 cM
Summary High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010170" in other vectors (4)
SKU Description Size Price
MC208462 F2rl2 (untagged) - Mouse coagulation factor II (thrombin) receptor-like 2 (F2rl2), (10ug) 10 ug
$503.00
MR216610 F2rl2 (Myc-DDK-tagged) - Mouse coagulation factor II (thrombin) receptor-like 2 (F2rl2) 10 ug
$289.00 $457.00
MR216610L3 Lenti ORF clone of F2rl2 (Myc-DDK-tagged) - Mouse coagulation factor II (thrombin) receptor-like 2 (F2rl2) 10 ug
$757.00
MR216610L4 Lenti ORF clone of F2rl2 (mGFP-tagged) - Mouse coagulation factor II (thrombin) receptor-like 2 (F2rl2) 10 ug
$757.00

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