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Rasa2 (NM_053268) Mouse Tagged ORF Clone

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SKU
MG216608
Rasa2 (tGFP-tagged) - Mouse RAS p21 protein activator 2 (Rasa2), (10ug)
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ORF Plasmid:
tGFP DDK
  $976.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rasa2
Synonyms 5430433H21Rik; AA517451; AU023900; GAP1m
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216608 representing NM_053268
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCAGCGCCTGCTGCGGCGGCTTCTCCCGAGGCGCCGGCCGTCTCGGGCAGCGCAGACCCGG
AGACGGGAGACGAGGACAGTCGTGAGGTCCGAGTGCTGCAGAGCCTGCGCGGCAGGATCTATGAAGCAAA
AAATTTATTGCCATATCTTGGACCCAACAAAATGCGCGATTGTTTCTGCACTATAAATTTGGACCAGGAA
GAAGTTTATCGCACCCAGGTTGTTGAAAAGTCTTTAAGTCCATATTTCAGTGAAGAATTTTATTTTGAGA
TTCCAAGAACTTTCCAGTATTTGTCTTTTTATGTTTATGATAAAAATGTTTTACAAAGAGATCTTCGTAT
AGGAAAAGTAGCCATCAAAAAAGAAGACTTGTGCAGTCATAGTGGTAAAGAAACTTGGTTTTCACTGCAG
CCGATTGACTCAAATTCAGAAGTTCAGGGAAAGGTTCACCTTGAGTTAAGACTGAATGAGCTGATAACAG
AGAATGGAACCGTGTGCCAGCAGCTTGTTGTACACATCAAGGCCTGCCATGGGTTGCCTCTCATAAACGG
ACAAAGCTGTGATCCTTATGCAACAGTGTCCCTCGTGGGCCCCTCTAGGAATGACCAAAAGAAGACAAAA
GTAAAGAAGAAAACAAGCAATCCACAGTTCAATGAAGTCTTCTATTTTGAGGTAACCAGATCCAGCAGTT
ACAGCAGGAAGTCCCAGTTCCAGGTGGAGGAGGAGGACATTGAGAAGCTGGAGATCAGGATTGACTTGTG
GAATAATGAAAACCTAGTCCAAGATGTTTTCCTGGGAGAGATTAAGGTTCCTGTCAATGTGTTAAGAAGT
GATTCCTTTCATCAAGCCTGGTACTTGCTACAGCCAAGAGACAATGGAAACAAATCATCTAAAACCGATG
ACCTGGGATCTCTTCTGTTAACTCTCTGCTATACAGAAGACTGCGTGCTTCCATCAGAGTACTATGGACC
GCTGAAAACTTTGCTGCTAAAGTCACCAGATGTTCAGCCAGTATCCGCCTCAGCTGCTTATATTTTGGGT
GAAATATGTCAAGATCAAAAGGATGCTGTTTTGCCCCTTGTACGACTGTTGCTGCATCACAATAAACTTG
TCCCTTTTATCACTGCGGTGGCCGAGCTGGACTTGAAGGATACTCCAGATGCCAACGCAATATTTAGAGG
AAACTCCCTGGCTACCCAGTGTCTGACTGAGATGATGAAGATAGTGGGAGGGCACTACCTGAAAGTCACG
CTGAAGCCCGTTCTAGATGAGATATGTGAATCCTCGAAATCCTGTGAAATAGATCCTGTTAAGTTGAAAG
AGGGAGATAATGTAGAGAATAATAAGGAGAATCTGTACTACTATGTGGACAAAGTATTCAATACAATCGT
AGGATCAAGTGTGAGCTGCCCCACTGTGATGTGTGATATTTTTTATTCTCTGAGGCAAATGGCTGCTAAG
AAATTTCCTAATCACCCTCATGTTCAGTACTCTGCAGTGAGCAGCTTTGTCTTCCTTCGTTTCTTTGCTG
TAGCGATACTCTCACCTCATGCTTTTCATTTGCGACCTCATTATCCAGATACACAGACAGTTAGGACATT
GACTCTCATCTCAAAAACCATTCAGATTATTGGAAACTGGGGATGTCAGTCCAGAAAAAAGTCACGGTTC
AAAAAGTCAGTCATGTGTGAATTTCTCAAAATGTTTCAAGAAGAAAGATATTTTACAGATGTGAAAAAGT
TCTTGGATGAAATTTCATCTACGGAAACTAAAGAGTCCAGTGGTACGAGTGAGCCTGTGCACCTGAAAGA
AGGTGAGATGTATAAGAGAGCTCAAGGAAGAACCCGTATTGGAAAGAAGAACTTCAAGAAACGATGGTTC
TGCTTGACGAGCAGAGAGCTCACCTACCACAGACAGCAAGGCAAAGATGCAATTTACACTATTCCAGTAA
AAAATATTCTTGCTGTGGAAAAATTGGAAGAAGGCTCTTTCAACAAGAAAAATATGTTCCAAGTGATACA
TACAGAGAAAACGCTGTACATCCAAGCAAACAACTGTGTGGAAGCCAATGAGTGGATAGATGTCCTGTGC
AGGGTGAGCAGATGCAACCACAACAGGCTCAGCTCTTTCCACCCCTCAGCGTATCTCAACGGAAACTGGC
TCTGCTGCCAGGAGACGAGTGAAAGCACACCAGGCTGCAAGCCGTGCACCGCAGGCATCCCTGCAGACAT
CCAGATAGATATCGATGAAGACAGAGAAACAGAAAGAATTTATTCTATTTTTACCCTCAGTTTACTTAAG
CTGCAGAAAATGGAAGAGACCTGTGGGTCTATAGCTGTGTATCAGGGGCCACAGAAAGAACCTGGTTATT
CTAAGTTCACCATTGAGGATTCTGTTGCAACCTTTAAGACAATTCAGCAAATCAAAAGCACCATAGAGAA
GCTCGATGAACCTCATGAGAAATACAGAAAGAAAAGATCGAGTAGTGCAAAATACGGAAGCAAGGAAAAT
CCAATTGTTGGAAAAATATCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216608 representing NM_053268
Red=Cloning site Green=Tags(s)

MAAAAPAAAASPEAPAVSGSADPETGDEDSREVRVLQSLRGRIYEAKNLLPYLGPNKMRDCFCTINLDQE
EVYRTQVVEKSLSPYFSEEFYFEIPRTFQYLSFYVYDKNVLQRDLRIGKVAIKKEDLCSHSGKETWFSLQ
PIDSNSEVQGKVHLELRLNELITENGTVCQQLVVHIKACHGLPLINGQSCDPYATVSLVGPSRNDQKKTK
VKKKTSNPQFNEVFYFEVTRSSSYSRKSQFQVEEEDIEKLEIRIDLWNNENLVQDVFLGEIKVPVNVLRS
DSFHQAWYLLQPRDNGNKSSKTDDLGSLLLTLCYTEDCVLPSEYYGPLKTLLLKSPDVQPVSASAAYILG
EICQDQKDAVLPLVRLLLHHNKLVPFITAVAELDLKDTPDANAIFRGNSLATQCLTEMMKIVGGHYLKVT
LKPVLDEICESSKSCEIDPVKLKEGDNVENNKENLYYYVDKVFNTIVGSSVSCPTVMCDIFYSLRQMAAK
KFPNHPHVQYSAVSSFVFLRFFAVAILSPHAFHLRPHYPDTQTVRTLTLISKTIQIIGNWGCQSRKKSRF
KKSVMCEFLKMFQEERYFTDVKKFLDEISSTETKESSGTSEPVHLKEGEMYKRAQGRTRIGKKNFKKRWF
CLTSRELTYHRQQGKDAIYTIPVKNILAVEKLEEGSFNKKNMFQVIHTEKTLYIQANNCVEANEWIDVLC
RVSRCNHNRLSSFHPSAYLNGNWLCCQETSESTPGCKPCTAGIPADIQIDIDEDRETERIYSIFTLSLLK
LQKMEETCGSIAVYQGPQKEPGYSKFTIEDSVATFKTIQQIKSTIEKLDEPHEKYRKKRSSSAKYGSKEN
PIVGKIS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053268
ORF Size 2541 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053268.2, NP_444498.2
RefSeq Size 5699 bp
RefSeq ORF 2544 bp
Locus ID 114713
UniProt ID P58069
Cytogenetics 9 E3.3
Summary Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4) and phospholipids.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_053268" in other vectors (4)
SKU Description Size Price
MC222069 Rasa2 (untagged) - Mouse RAS p21 protein activator 2 (Rasa2), (10ug) 10 ug
$854.00
MR216608 Rasa2 (Myc-DDK-tagged) - Mouse RAS p21 protein activator 2 (Rasa2) 10 ug
$589.00 $776.00
MR216608L3 Lenti ORF clone of Rasa2 (Myc-DDK-tagged) - Mouse RAS p21 protein activator 2 (Rasa2) 10 ug
$1,076.00
MR216608L4 Lenti ORF clone of Rasa2 (mGFP-tagged) - Mouse RAS p21 protein activator 2 (Rasa2) 10 ug
$1,076.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.