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Pde4a (NM_183408) Mouse Tagged ORF Clone

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SKU
MG216607
Pde4a (tGFP-tagged) - Mouse phosphodiesterase 4A cAMP specific (Pde4a) transcript variant 5, (10ug)
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ORF Plasmid:
tGFP DDK
  $974.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pde4a
Synonyms D9Ertd60e; Dpde2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216607 representing NM_183408
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCTCCGGCCGCCCCCTCGGAGAGGAGCCTGTCTCTCTCTCTTCCCGGGCCCCGAGAGGGCCAGG
CCACCCTGAAGCCGCCCCCCCAGCACCTGTGGCGGCAGCCGAGGACCCCGATCCGCATCCAGCAGCGCGG
CTACTCGGACAGTGCCGAGCGCTCAGAGCCAGAGCGCTCGCCGCACCGGCCCATAGAGCGCGCCGACGCC
GTGGACACTGGCGACCGGCCAGGCCTGCGGACTACCCGCATGTCCTGGCCCTCGTCCTTCCACGGCACCG
GTACCGGCGGAGGCAGCAGTAGGCGCTTGGAGGCAGAAAATGGGCCAACACCGTCCCCTGGCCGCAGCCC
CCTGGACTCGCAGGCGAGCCCGGGGCTTATGCTGCATGCCGGGGCAGCCACCAGCCAGCGCCGAGAATCC
TTCCTCTATCGCTCAGACAGCGACTATGACATGTCACCGAAGACTATGTCCAGGAACTCATCCGTCGCCA
GCGAAGCGCATGGTGAAGACCTCATTGTGACCCCGTTTGCCCAGGTGCTGGCCAGTCTCCGCAACGTTCG
AAGCAACTTCTCACTCCTAACCAATGTGCCCATCCCCAGCAACAAGAGGTCCCCGCTGGGCGGGCCACCC
TCTGTCTGCAAGGCCACACTGTCAGAGGAGACATGCCAGCAGCTGGCCCGGGAGACCCTGGAAGAGCTGG
ATTGGTGCCTGGAGCAGCTGGAGACCATGCAGACCTACCGCTCTGTCAGCGAGATGGCCTCACACAAGTT
TAAAAGGATGCTGAACCGGGAACTCACACACCTGTCGGAAATGAGCAGGTCAGGAAACCAGGTCTCAGAG
TACATTTCCAACACGTTCCTAGACAAGCAGCACGAAGTGGAGATCCCATCGCCCACGCCGCGACAGAGAC
CCTTCCAGCAGCCCCCACCAGCAGCAGTGCAGCAGGCCCAGCCCATGTCTCAGATCACAGGGCTGAAAAA
GCTGGTACACACCGGAAGCTTGAACATCAATGTCCCACGATTTGGAGTCAAGACAGATCAGGAGGACCTC
TTAGCACAAGAACTGGAGAACTTGAGCAAATGGGGCCTGAACATCTTTTGTGTGTCGGAGTACGCTGGAG
GCCGTTCACTCAGCTGTATCATGTATACGATATTCCAGGAGCGGGACTTACTGAAGAAATTCCACATCCC
CGTGGACACCATGATGACATACATGCTGACGCTGGAGGACCACTACCACGCTGACGTGGCCTACCACAAC
AGCCTGCACGCAGCCGATGTGCTACAGTCCACACATGTGCTGCTGGCCACGCCTGCACTGGATGCCGTGT
TCACAGACCTGGAGATTCTTGCTGCTCTCTTCGCTGCTGCCATCCATGACGTGGACCACCCTGGCGTCTC
CAACCAGTTCCTAATCAACACCAATTCGGAGCTGGCATTGATGTACAACGATGAGTCTGTGCTTGAGAAC
CACCACCTGGCTGTGGGATTCAAGCTGCTGCAAGAAGAGAACTGCGACATCTTCCAGAACCTCAGCAAGC
GCCAGCGGCAGAGCCTGCGCAAGATGGTCATCGACATGGTGCTGGCCACAGACATGTCCAAGCACATGAC
CCTTCTGGCTGACCTGAAGACTATGGTAGAGACGAAGAAAGTGACCAGCTCTGGAGTTCTCTTGCTGGAC
AACTACTCTGACCGTATCCAGGTCCTCAGGAACATGGTGCACTGTGCAGACCTCAGCAATCCCACCAAGC
CCCTGGAGCTGTACCGACAATGGACTGATCGCATCATGGCTGAGTTCTTCCAGCAGGGTGACCGAGAACG
GGAACGCGGAATGGAGATTAGCCCCATGTGTGACAAGCACACAGCCTCTGTGGAGAAGTCTCAGGTGGGC
TTCATCGACTATATTGTTCACCCACTGTGGGAGACGTGGGCCGATCTCGTCCACCCCGATGCCCAAGACA
TCCTGGACACGCTGGAAGACAACCGGGATTGGTATCACAGTGCCATCCGCCAGAGTCCCTCCCCAACCCT
GGAAGAGGAGCCAGGAGTGCTCAGCGATCCGGCCCTGCCTGACAAGTTCCAATTTGAGCTCACCTTGGAG
GAAGAGGATGAAGAGGATTCATTGGAGGTTCCAGGATTGCCTTGCACTGAGGAAACTCTCCTGGCTCCAC
ATGATACCAGAGCTCAAGCCATGGAACAGTCAAAGGTCAAAGGCCAGAGCCCTGCTGTTGTGGAGGTAGC
AGAGAGTTTGAAGCAGGAGACAGCCTCAGCACATGGCGCTCCTGAGGAGTCCGCGGAGGCTGTGGGCCAT
TCCTTCAGCCTTGAAACCTCTATTCTGCCTGACTTGAGGACCCTGTCCCCCTCAGAGGAGGCCCAGGGCC
TCCTGGGCCTCCCCTCCATGGCGGCAGAGGTGGAGGCCCCAAGAGACCATCTGGCTGCCATGAGGGCTTG
TTCTGCCTGCTCGGGGACATCAGGAGACAATTCTGCCGTCATCTCTGCTCCAGGCAGGTGGGGGTCAGGT
GGAGACCCTGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216607 representing NM_183408
Red=Cloning site Green=Tags(s)

MEPPAAPSERSLSLSLPGPREGQATLKPPPQHLWRQPRTPIRIQQRGYSDSAERSEPERSPHRPIERADA
VDTGDRPGLRTTRMSWPSSFHGTGTGGGSSRRLEAENGPTPSPGRSPLDSQASPGLMLHAGAATSQRRES
FLYRSDSDYDMSPKTMSRNSSVASEAHGEDLIVTPFAQVLASLRNVRSNFSLLTNVPIPSNKRSPLGGPP
SVCKATLSEETCQQLARETLEELDWCLEQLETMQTYRSVSEMASHKFKRMLNRELTHLSEMSRSGNQVSE
YISNTFLDKQHEVEIPSPTPRQRPFQQPPPAAVQQAQPMSQITGLKKLVHTGSLNINVPRFGVKTDQEDL
LAQELENLSKWGLNIFCVSEYAGGRSLSCIMYTIFQERDLLKKFHIPVDTMMTYMLTLEDHYHADVAYHN
SLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLEN
HHLAVGFKLLQEENCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLD
NYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVG
FIDYIVHPLWETWADLVHPDAQDILDTLEDNRDWYHSAIRQSPSPTLEEEPGVLSDPALPDKFQFELTLE
EEDEEDSLEVPGLPCTEETLLAPHDTRAQAMEQSKVKGQSPAVVEVAESLKQETASAHGAPEESAEAVGH
SFSLETSILPDLRTLSPSEEAQGLLGLPSMAAEVEAPRDHLAAMRACSACSGTSGDNSAVISAPGRWGSG
GDPA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183408
ORF Size 2532 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183408.3, NP_899668.1
RefSeq Size 4658 bp
RefSeq ORF 2535 bp
Locus ID 18577
UniProt ID O89084
Cytogenetics 9 7.73 cM
Summary Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_183408" in other vectors (4)
SKU Description Size Price
MC221997 Pde4a (untagged) - Mouse phosphodiesterase 4A, cAMP specific (Pde4a), transcript variant 5, (10ug) 10 ug
$851.00
MR216607 Pde4a (Myc-DDK-tagged) - Mouse phosphodiesterase 4A, cAMP specific (Pde4a), transcript variant 5 10 ug
$589.00 $774.00
MR216607L3 Lenti ORF clone of Pde4a (Myc-DDK-tagged) - Mouse phosphodiesterase 4A, cAMP specific (Pde4a), transcript variant 5 10 ug
$1,074.00
MR216607L4 Lenti ORF clone of Pde4a (mGFP-tagged) - Mouse phosphodiesterase 4A, cAMP specific (Pde4a), transcript variant 5 10 ug
$1,074.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.