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Agbl4 (NM_030231) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216595
Agbl4 (tGFP-tagged) - Mouse ATP/GTP binding protein-like 4 (Agbl4) transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $703.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Agbl4
Synonyms 4930578N11Rik; 4931433A01Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216595 representing NM_030231
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGCGGAGCCAGACAGCGCCAGAGGCAGGCAATGATACAGGAAATGAGGATGCCATTGGAGGGA
ATGTGAACAAATACATAGTGCTTCCAAATGGATACTCTGGACAGCCCAAGAAAGGACATCTTACCTTTGA
TGCTTGCTTTGAAAGTGGTAACCTCGGCCGGGTTGAGCAAGTCTCTGATTTTGAGTATGATCTGTTCATT
AGGCCGGACACCTGTAATCCACGCTTCCGAGTCTGGTTCAACTTTACTGTTGAAAATGTGAAAGAATTGC
AGAGGGTAATTTTCAACATTGTTAACTTCAGTAAAACCAAGAGTCTTTACCGAGATGGGATGGCACCAAT
GGTGAAATCTACCAGCAGACCAAAATGGCAAAGACTACCACCAAAAAACGTTTATTACTATCGCTGCCCA
GACCACAGGAAGAACTATGTGATGTCCTTTGCATTCTGTTTTGACCGAGAAGATGATATCTACCAGTTTG
CTTACTGCTACCCTTATACATATACTCGCTTCCAGCATTACCTCGACAGCTTACAAAAGAAAAACATGGA
TTATTTCTTCCGGGAACAGCTTGGACAGAGTGTGCAACAGCGGCAGCTTGACCTCCTGACGATAACCAGC
CCGGAGAATCTCCGTGAAGGGTCAGAAAAGAAGGTGATATTCATCACAGGGCGAGTCCACCCAGGGGAAA
CGCCATCTTCATTTGTGTGCCAAGGAATCATCGACTTCCTTGTAAGTCAGCATCCAATTGCCCGTGTCCT
ACGAGAACATTTAGTCTTCAAGATTGCTCCAATGCTCAACCCTGATGGAGTTTACCTGGGCAACTACAGG
TGTTCCCTGATGGGGTTTGACCTGAATCGTCACTGGCTGGATCCCTCTCCATGGGCCCATCCCACCCTGC
ATGGAGTGAAACAGCTTATTATCAAGATGTACAATGACCCAAAAACAAGCCTGGAGTTCTATATTGACAT
CCACGCTCACTCCACCATGATGAATGGCTTCATGTACGGCAATATCTTTGAGGATGAGGAACGGTTCCAA
AGGCAGTCCATTTTTCCAAAACTCCTTTGCCAGAATGCCGAGGACTTCTCCTATACTAGCACATCCTTCA
ACAGGGATGCTGTGAAAGCAGGAACTGGCCGGCGCTTCCTGGGCGGGCTTCTGGACCACTCATCATACTG
CTATACCCTAGAGGTTTCCTTCTACAGCTACATCATTGGGGGTACTACAGCTGCAGTGCCCTACACTGAA
GAAGCCTATATGAAACTGGGACGGAACGTGGCAAGAACATTTCTAGATTATTACCGGCTGAACTCCCTGG
TTGAGAAGATAGCAGTCCCCATGCCAAGACTTCGATCCAAGGAGGAAAGAAGACTGGGATGGGAGCATCC
ATCTTGTCTGAGGGCTGAACAACCACTGGAAGTGCTGGGAATTCCCATGTGTTCTGGTAAAGCACTCAAT
GAGCATCTTGGCAATGACATCCAGTGGCATCTGGATTGTGGTAGCAGTACCCTACCTCTGGGCCTCATCT
CCTGTTCCCCTAGTAGCTCAGCCTCCTGGAATGACATGGCAATGTCCAACTCTATACTCCTGCCAGACCA
TTCTTTTCAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216595 representing NM_030231
Red=Cloning site Green=Tags(s)

MAERSQTAPEAGNDTGNEDAIGGNVNKYIVLPNGYSGQPKKGHLTFDACFESGNLGRVEQVSDFEYDLFI
RPDTCNPRFRVWFNFTVENVKELQRVIFNIVNFSKTKSLYRDGMAPMVKSTSRPKWQRLPPKNVYYYRCP
DHRKNYVMSFAFCFDREDDIYQFAYCYPYTYTRFQHYLDSLQKKNMDYFFREQLGQSVQQRQLDLLTITS
PENLREGSEKKVIFITGRVHPGETPSSFVCQGIIDFLVSQHPIARVLREHLVFKIAPMLNPDGVYLGNYR
CSLMGFDLNRHWLDPSPWAHPTLHGVKQLIIKMYNDPKTSLEFYIDIHAHSTMMNGFMYGNIFEDEERFQ
RQSIFPKLLCQNAEDFSYTSTSFNRDAVKAGTGRRFLGGLLDHSSYCYTLEVSFYSYIIGGTTAAVPYTE
EAYMKLGRNVARTFLDYYRLNSLVEKIAVPMPRLRSKEERRLGWEHPSCLRAEQPLEVLGIPMCSGKALN
EHLGNDIQWHLDCGSSTLPLGLISCSPSSSASWNDMAMSNSILLPDHSFH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030231
ORF Size 1620 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030231.3, NP_084507.1
RefSeq Size 1798 bp
RefSeq ORF 1623 bp
Locus ID 78933
UniProt ID Q09LZ8
Cytogenetics 4 C7-D1
Summary Metallocarboxypeptidase that mediates deglutamylation of target proteins (PubMed:17244818, PubMed:21074048, PubMed:25103237, PubMed:26829768). Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins (PubMed:17244818). Also removes polyglutamates from the carboxy-terminus of target proteins such as MYLK (PubMed:21074048). Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS (PubMed:26829768). Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5 (PubMed:25103237).UniProtKB/Swiss-Prot Function
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"NM_030231" in other vectors (4)
SKU Description Size Price
MC218499 Agbl4 (untagged) - Mouse ATP/GTP binding protein-like 4 (Agbl4), transcript variant 1, (10ug) 10 ug
$554.00
MR216595 Agbl4 (Myc-DDK-tagged) - Mouse ATP/GTP binding protein-like 4 (Agbl4), transcript variant 1 10 ug
$289.00 $503.00
MR216595L3 Lenti ORF clone of Agbl4 (Myc-DDK-tagged) - Mouse ATP/GTP binding protein-like 4 (Agbl4), transcript variant 1 10 ug
$803.00
MR216595L4 Lenti ORF clone of Agbl4 (mGFP-tagged) - Mouse ATP/GTP binding protein-like 4 (Agbl4), transcript variant 1 10 ug
$803.00

Plasmid Map for MG216595

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG216595
Circular map for MG216595

file-alt Citations

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