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Msl2 (NM_001100451) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216585
Msl2 (tGFP-tagged) - Mouse male-specific lethal 2 homolog (Drosophila) (Msl2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $737.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Msl2
Synonyms E130103E02Rik; Msl2l1; Rnf184
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216585 representing NM_001100451
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCCGGTGAATGCTACTGCTCTCTACATTTCCGCGAGCCGCCTAGTGCTCAACTACGACCCCGGAG
ACCCCAAGGCGTTTACAGAGATTAACAGGCTCTTGCCTTACTTCCGACAGTCCCTCTCGTGCTGTGTTTG
CGGACATTTGCTTCAAGATCCTATTGCACCCACCAACTCCACCTGCCAACACTATGTCTGCAAAACTTGT
AAAGGCAAGAAAATGATGATGAAACCTTCATGTAGCTGGTGCAAAGACTATGAGCAGTTTGAGGAGAACA
AGCAGTTAAGCATCCTAGTGAATTGCTACAAGAAACTGTGTGAATATATAACCCAGACGACACTGGCACG
GGATATAATAGAAGCAGTTGACTGTTCTTCTGATATTTTGGCTTTGCTTAATGACGGATCATTGTTTTGT
GAGGAGACCGAGAAACCTTCAGATTCATCCTTCACTCTGTGTTTGACACATTCCCCTTTACCTTCAACTT
CAGAACCCACAGCTGATCCTCAAGCTAGCTTATCTCCAATGTCTGAGAGTACCCTCAGCATTGCCATTGG
CAGTTCTGTTATCAATGGTTTGCCTACTTACAATGGGCTTTCAATAGATAGATTTGGTATAAATATCCCT
TCACCTGAACATCCAAATACAATTGACGTGTGTAATACTGTTGATATAAAAACTGAGGATCTGTCTGACA
ACCTGCCACCTGTCTGTGACACGGTAGCCACTGACTTGTGCTCCACAGGTATTGATATCTGTAGTTTCAG
TGAAGATATAAAACCCGGTGACTCTCTTTTGCTGAGTGTTGAGGAAGTACTCCGCAGCTTAGAAACTGTT
TCAAATACAGAAGTTTGCTGTCCTAATTTGCAGCCAAACTTGGAAGCCACTGTATCCAATGGACCTTTTT
TGCAGCTTTCTTCCCAGTCTCTTAGCCATAATGTTTTCATGTCCACCAGCCCTGCACTTCATGGGTTATC
ATGTACAGCAGCAACTCCCAAGGTAGCAAAGTTGAATCGAAAACGATCCAGATCTGAAAGTGACAGTGAG
AAAGTTCAGCCACTTCCAATTTCTACCATTATCCGAGGCCCAACACTGGGGGCATCTGCTCCTGTGACTG
TGAAGCGGGAAAGCAAAATCTCTCTTCAACCTATAGCAACTGTTCCCAATGGAGGCACGACACCCAAGAT
CAGCAAGACTGTACTTTTATCTACTAAAAGCATGAAAAAGAGTCATGAACATGGATCCAAGAAATCTCAC
TCTAAATCCAAGCCAGGTATTCTTAAAAAAGACAAAGCAGTAAAGGAAAAGATGCCTAGTCATCATTTTA
TGCCAGGAAGCCCTACCAAGACTGTGTACAAAAAGCCCCAGGAAAAGAAAGGATGTAAATGTGGGCGTGC
TACTCAAAATCCAAGTGTTCTTACATGCCGCGGCCAACGCTGCCCTTGCTACTCTAACCGGAAAGCCTGC
TTAGATTGTATATGTCGTGGCTGCCAAAACTCCTATATGGCCAATGGGGAGAAGAAGCTGGAGGCATTTG
CGGTGCCAGAAAAGGCCTTGGAGCAGACCAGGCTCACTTTGGGCATTAATGTGACTAGCATTGCTGTGCG
TAACGCAAGTACCAGCACCAGTGTAATTAATGTCACAGGGTCCCCTGTAACGACATTTTTGGCTGCCAGT
ACACATGATGATAAAAGTTTGGATGAAGCTATAGATATGAGATTCGACTGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216585 representing NM_001100451
Red=Cloning site Green=Tags(s)

MNPVNATALYISASRLVLNYDPGDPKAFTEINRLLPYFRQSLSCCVCGHLLQDPIAPTNSTCQHYVCKTC
KGKKMMMKPSCSWCKDYEQFEENKQLSILVNCYKKLCEYITQTTLARDIIEAVDCSSDILALLNDGSLFC
EETEKPSDSSFTLCLTHSPLPSTSEPTADPQASLSPMSESTLSIAIGSSVINGLPTYNGLSIDRFGINIP
SPEHPNTIDVCNTVDIKTEDLSDNLPPVCDTVATDLCSTGIDICSFSEDIKPGDSLLLSVEEVLRSLETV
SNTEVCCPNLQPNLEATVSNGPFLQLSSQSLSHNVFMSTSPALHGLSCTAATPKVAKLNRKRSRSESDSE
KVQPLPISTIIRGPTLGASAPVTVKRESKISLQPIATVPNGGTTPKISKTVLLSTKSMKKSHEHGSKKSH
SKSKPGILKKDKAVKEKMPSHHFMPGSPTKTVYKKPQEKKGCKCGRATQNPSVLTCRGQRCPCYSNRKAC
LDCICRGCQNSYMANGEKKLEAFAVPEKALEQTRLTLGINVTSIAVRNASTSTSVINVTGSPVTTFLAAS
THDDKSLDEAIDMRFDC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001100451
ORF Size 1731 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001100451.2, NP_001093921.1
RefSeq Size 4934 bp
RefSeq ORF 1734 bp
Locus ID 77853
UniProt ID Q69ZF8
Cytogenetics 9 E4
Summary Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at lysine 16 which is implicated in the formation of higher-order chromatin structure (By similarity). Acts as an E3 ubiquitin ligase that promotes monoubiquitination of histone H2B at 'Lys-35' (H2BK34Ub), but not that of H2A. This activity is greatly enhanced by heterodimerization with MSL1. H2B ubiquitination in turn stimulates histone H3 methylation at 'Lys-5' (H3K4me) and 'Lys-80' (H3K79me) and leads to gene activation, including that of HOXA9 and MEIS1 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001100451" in other vectors (4)
SKU Description Size Price
MC219204 Msl2 (untagged) - Mouse male-specific lethal 2 homolog (Drosophila) (Msl2), (10ug) 10 ug
$592.00
MR216585 Msl2 (Myc-DDK-tagged) - Mouse male-specific lethal 2 homolog (Drosophila) (Msl2) 10 ug
$289.00 $537.00
MR216585L3 Lenti ORF clone of Msl2 (Myc-DDK-tagged) - Mouse male-specific lethal 2 homolog (Drosophila) (Msl2) 10 ug
$837.00
MR216585L4 Lenti ORF clone of Msl2 (mGFP-tagged) - Mouse male-specific lethal 2 homolog (Drosophila) (Msl2) 10 ug
$837.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.