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Mrgprx2 (NM_001034868) Mouse Tagged ORF Clone

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SKU
MG216537
Mrgprx2 (tGFP-tagged) - Mouse MAS-related GPR member X2 (Mrgprx2), (10ug)
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ORF Plasmid:
tGFP DDK
  $886.00
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Other products for "Mrgprx2 or NM_001034868"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mrgprx2
Synonyms G370024M05Rik; MrgB10; Mrgprb10
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216537 representing NM_001034868
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAAAGAAACATCAGTGGAAGGGATCTAAGAGTGGATTCAAACATAACATACTGGGGAACAAACA
TCACAGCTGTGAATGAAAGCAACCATACTGGAATGTCATTTTGTGAAGTCGTGTCTTGTACCATGGTTTT
TCTTTCCCTCATTGTTGCCCTAGTTGGGCTGGTTGGAAATGCCACAGTGCTGTGGTTCCTGGGCTTCCAG
ATGCGCAGGAATGCATTCTCTGTTTACATCCTCAACCTCGCTGGTGCTGACTTTCTCTTCATTTGCTTTC
AAATTGGATATTGTTTTCACATGATCTTGGACATTGATTCCATCCCCATTGAAATTGATCTGTTTTACCT
TGTTGTGTTAAACTTTCCTTATTTTTGTGGCCTGAGTATCCTCAGTGCTATTAGCATTGAACGTTGCCTG
TCTGTCATGTGGCCCATTTGGTATCACTGCCAACGCCCAAGGCACACATCAGCTGTCATATGTACCCTGC
TTTGGGTCTTGTCCCTAGTGTGTAGCCTCCTGGAAGGGAAGGAATGTGGCTTCCTATATTACACTAGTGA
CCCTGGTTGGTGTAAGACATTTGATTTAATCACTGCTACATGGTTAATTGTTTTATTTGTAGCTCTCTTG
GGATCCAGTCTGGCCTTAGTGATTACCATCTTCTGGGGCTTACACAAGATTCCTGTGACCAGGCTGTATG
TGGCCATTGTGTTCACAGTGCTTGTTTTCCTGCTCTTTGGTCTGCCCTATGGGATCTACTGGTTCCTCTT
AGTGTGGATTGAGAAATTTTATTATGTTTTACCTTGTAGTATATATCCGGTCACAGTATTTCTCTCCTGT
GTTAACAGCTCTGCAAAACCCATCATTTATTGCCTTGTAGGCTCCATTAGGCATCATCGATTTCAACGGA
AGACTCTCAAGCTATTTCTGCAGAGAGCCATGCAAGACACTCCTGAGGAGGAAGAATGTGGAGAGATGGG
TTCCTCAGGAAGATCTAGAGAAATAAAAACAATCTGGAAAGGACTGAGAGCTGCTTTGATCAGGCATAAA
GAGCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216537 representing NM_001034868
Red=Cloning site Green=Tags(s)

MEERNISGRDLRVDSNITYWGTNITAVNESNHTGMSFCEVVSCTMVFLSLIVALVGLVGNATVLWFLGFQ
MRRNAFSVYILNLAGADFLFICFQIGYCFHMILDIDSIPIEIDLFYLVVLNFPYFCGLSILSAISIERCL
SVMWPIWYHCQRPRHTSAVICTLLWVLSLVCSLLEGKECGFLYYTSDPGWCKTFDLITATWLIVLFVALL
GSSLALVITIFWGLHKIPVTRLYVAIVFTVLVFLLFGLPYGIYWFLLVWIEKFYYVLPCSIYPVTVFLSC
VNSSAKPIIYCLVGSIRHHRFQRKTLKLFLQRAMQDTPEEEECGEMGSSGRSREIKTIWKGLRAALIRHK
EL

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001034868
ORF Size 1056 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001034868.3, NP_001030040.2
RefSeq Size 4559 bp
RefSeq ORF 1059 bp
Locus ID 243978
UniProt ID Q3UG50
Cytogenetics 7 B4
Summary Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001034868" in other vectors (4)
SKU Description Size Price
MC213182 Mrgprx2 (untagged) - Mouse MAS-related GPR, member X2 (Mrgprx2), (10ug) 10 ug
$686.00
MR216537 Mrgprx2 (Myc-DDK-tagged) - Mouse MAS-related GPR, member X2 (Mrgprx2) 10 ug
$686.00
MR216537L3 Lenti ORF clone of Mrgprx2 (Myc-DDK-tagged) - Mouse MAS-related GPR, member X2 (Mrgprx2) 10 ug
$986.00
MR216537L4 Lenti ORF clone of Mrgprx2 (mGFP-tagged) - Mouse MAS-related GPR, member X2 (Mrgprx2) 10 ug
$986.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.