Trim7 (NM_053166) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG216528
Trim7 (tGFP-tagged) - Mouse tripartite motif-containing 7 (Trim7), (10ug)
$489.00 $500.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trim7
Synonyms AI790312
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216528 representing NM_053166
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCAGAGAAAGAGAAGGTAGGGGCGGAGTTCCAAGCGCTGCGGGCCTTCCTGGTGGAGCAGGAGG
GTCGGCTTCTAAGCCGACTGGAGGTGCTGTCCCGGGAAGTGACACAGAAACAGAATGAGAACCTGGCCCA
GCTGGAGGGTGAGATCACTCAGCTGTCCAAACTCAGCGGCCAGATCCAGGAGACAGCTCAGAAGCCGGAC
CTAGACTTCCTCCAGGAGTTCAAAAGCACACTGAGCAAGTGCAGCAGCGTGCCCAGCTCCAAGCCAACCA
CAGTCTCATCTGAGATGAAGAATAAAGTGTGGAATGTTTCCCTCAAGAGTTTCGTCTTAAAAGGATTGCT
GAAGAAGTTCAAAGAGGATCTCCAGGGAGAGCTGGAGAAAGAAGAGAAAGTGGAACTCACTTTGGATCCA
GACACAGCCAACCCCCGTCTCATCTTGTCCCTGGATCTAAAGAGCGTACGTCTAGGACAGCGCGCCCAGG
ACTTGCCTAACCATCCTCGCCGCTTTGATACCAACACCCGCGTTCTGGCATCCTGTGGTTTCTCCTCTGG
GCGACATCACTGGGAGGTGGAAGTGGGCTCCAAGGACGGCTGGGCTTTCGGTGTGGCCCGGGAGAGCGTG
CGTCGCAAGGGCCTCACGCCTTTTACCCCCGAGGAGGGCGTCTGGGCAATGCAACTCAACAATGGGCAAT
ACTGGGCGGTGACCAGCCCCGAGAGGACACAGCTCAACTGTGGGCACTTGTCGCGGGTGAGGGTGGCGCT
GGACCTTGAGGTGGGAGCAGTGTCCTTCTATGCAGTGGAGGACATGCGCCACCTCTACACCTTCCGCGTC
AACTTCCAGGAGCGAGTGTTCCCCCTTTTCTCTGTTTGTTCTACCGGCACATACTTGAGAATCTGGCCT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216528 representing NM_053166
Red=Cloning site Green=Tags(s)

MAAEKEKVGAEFQALRAFLVEQEGRLLSRLEVLSREVTQKQNENLAQLEGEITQLSKLSGQIQETAQKPD
LDFLQEFKSTLSKCSSVPSSKPTTVSSEMKNKVWNVSLKSFVLKGLLKKFKEDLQGELEKEEKVELTLDP
DTANPRLILSLDLKSVRLGQRAQDLPNHPRRFDTNTRVLASCGFSSGRHHWEVEVGSKDGWAFGVARESV
RRKGLTPFTPEEGVWAMQLNNGQYWAVTSPERTQLNCGHLSRVRVALDLEVGAVSFYAVEDMRHLYTFRV
NFQERVFPLFSVCSTGTYLRIWP

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053166
ORF Size 909 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053166.2, NP_444396.2
RefSeq Size 1366 bp
RefSeq ORF 912 bp
Locus ID 94089
UniProt ID Q923T7
Cytogenetics 11 B1.2
Summary E3 ubiquitin-protein ligase (By similarity). Mediates 'Lys-63'-linked polyubiquitination and stabilization of the JUN coactivator RNF187 in response to growth factor signaling via the MEK/ERK pathway, thereby regulating JUN transactivation and cellular proliferation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_053166" in other vectors (4)
SKU Description Size Price
MC212007 Trim7 (untagged) - Mouse tripartite motif-containing 7 (Trim7), (10ug) 10 ug
$330.00
MR216528 Trim7 (Myc-DDK-tagged) - Mouse tripartite motif-containing 7 (Trim7) 10 ug
$289.00 $300.00
MR216528L3 Lenti ORF clone of Trim7 (Myc-DDK-tagged) - Mouse tripartite motif-containing 7 (Trim7) 10 ug
$600.00
MR216528L4 Lenti ORF clone of Trim7 (mGFP-tagged) - Mouse tripartite motif-containing 7 (Trim7) 10 ug
$600.00

Plasmid Map for MG216528

Download sequence for

Cloning scheme for MG216528

Circular map for MG216528

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