Trim7 (NM_053166) Mouse Untagged Clone

SKU
MC212007
Trim7 (untagged) - Mouse tripartite motif-containing 7 (Trim7), (10ug)
  $330.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Trim7
Synonyms AI790312
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC212007 representing NM_053166
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCAGAGAAAGAGAAGGTAGGGGCGGAGTTCCAAGCGCTGCGGGCCTTCCTGGTGGAGCAGGAGG
GTCGGCTTCTAAGCCGACTGGAGGTGCTGTCCCGGGAAGTGACACAGAAACAGAATGAGAACCTGGCCCA
GCTGGAGGGTGAGATCACTCAGCTGTCCAAACTCAGCGGCCAGATCCAGGAGACAGCTCAGAAGCCGGAC
CTAGACTTCCTCCAGGAGTTCAAAAGCACACTGAGCAAGTGCAGCAGCGTGCCCAGCTCCAAGCCAACCA
CAGTCTCATCTGAGATGAAGAATAAAGTGTGGAATGTTTCCCTCAAGAGTTTCGTCTTAAAAGGATTGCT
GAAGAAGTTCAAAGAGGATCTCCAGGGAGAGCTGGAGAAAGAAGAGAAAGTGGAACTCACTTTGGATCCA
GACACAGCCAACCCCCGTCTCATCTTGTCCCTGGATCTAAAGAGCGTACGTCTAGGACAGCGCGCCCAGG
ACTTGCCTAACCATCCTCGCCGCTTTGATACCAACACCCGCGTTCTGGCATCCTGTGGTTTCTCCTCTGG
GCGACATCACTGGGAGGTGGAAGTGGGCTCCAAGGACGGCTGGGCTTTCGGTGTGGCCCGGGAGAGCGTG
CGTCGCAAGGGCCTCACGCCTTTTACCCCCGAGGAGGGCGTCTGGGCAATGCAACTCAACAATGGGCAAT
ACTGGGCGGTGACCAGCCCCGAGAGGACACAGCTCAACTGTGGGCACTTGTCGCGGGTGAGGGTGGCGCT
GGACCTTGAGGTGGGAGCAGTGTCCTTCTATGCAGTGGAGGACATGCGCCACCTCTACACCTTCCGCGTC
AACTTCCAGGAGCGAGTGTTCCCCCTTTTCTCTGTTTGTTCTACCGGCACATACTTGAGAATCTGGCCTT
GA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_053166
Insert Size 912 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053166.2, NP_444396.2
RefSeq Size 1366 bp
RefSeq ORF 912 bp
Locus ID 94089
UniProt ID Q923T7
Cytogenetics 11 B1.2
Summary E3 ubiquitin-protein ligase (By similarity). Mediates 'Lys-63'-linked polyubiquitination and stabilization of the JUN coactivator RNF187 in response to growth factor signaling via the MEK/ERK pathway, thereby regulating JUN transactivation and cellular proliferation (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 1, which is shorter at the N-terminus, compared to isoform 2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_053166" in other vectors (4)
SKU Description Size Price
MG216528 Trim7 (tGFP-tagged) - Mouse tripartite motif-containing 7 (Trim7), (10ug) 10 ug
$489.00 $500.00
MR216528 Trim7 (Myc-DDK-tagged) - Mouse tripartite motif-containing 7 (Trim7) 10 ug
$289.00 $300.00
MR216528L3 Lenti ORF clone of Trim7 (Myc-DDK-tagged) - Mouse tripartite motif-containing 7 (Trim7) 10 ug
$600.00
MR216528L4 Lenti ORF clone of Trim7 (mGFP-tagged) - Mouse tripartite motif-containing 7 (Trim7) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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