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Timd2 (NM_001161355) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216527
Timd2 (tGFP-tagged) - Mouse T-cell immunoglobulin and mucin domain containing 2 (Timd2) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $650.00
In Stock*
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Other products for "Timd2 or NM_001161355"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Timd2
Synonyms AA816106; AU019358; AU019662; C78111; G9D3; TIM-2; Tim2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216527 representing NM_001161355
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCAGATTCAAGTCTTCATTTCAGGCCTCATACTGCTTCTCCCAGGTGCCGTGGAGTCTCATACAG
CAGTGCAGGGGCTGGCGGGTCACCCTGTCACACTTCCATGTATTTATTCGACACACCTTGGTGGAATCGT
TCCTATGTGTTGGGGCCTAGGGGAATGCCGCCATTCTTATTGTATACGGTCACTTATCTGGACCAATGGA
TATACGGTCACACATCAGAGGAACAGTCTATACCAGCTAAAGGGGAATATTTCAGAAGGAAATGTGTCCT
TGACCATAGAGAACACTGTTGTGGGTGATGGTGGTCCCTATTGCTGTGTAGTGGAGATACCTGGAGCGTT
CCATTTTGTGGACTATATGTTGGAAGTTAAACCAGAAATTTCCACGAGTCCACCAACAAGGCCCACAGCT
ACAGGAAGACCCACAACTATTTCAACAAGATCCACACATGTACCAACATCAACCAGAGTCTCTACCTCTA
CTTCTCCAACACCAGCACACACAGAGACCTACAAACCAGAGGCCACTACATTTTATCCAGATCAGACTAC
AGCTGAGGTGACAGAAACCTTACCCGATACTCCTGCAGACTGGCATAACACTGTGACATCCTCAGATGAC
CCTTGGGATGATAACACTGAAGTAATCCCTCCACAGAAGCCACAGAAAAACCTGAATAAGGGCTTCTATG
TTGGCATCTCCATTGCAGCCCTGCTGATATTGATGCTTCTGAGCACCATGGTTATCACCAGGTACGTGGT
TATGAAAAGGAAGTCAGAATCTCTGAGCTTCGTTGCCTTCCCTATCTCTAAGATTGGAGCTTCCCCCAAA
AAAGTGGTCGAACGGACCAGATGTGAAGACCAGGTCTACATTATTGAAGACACTCCTTACCCTGAAGAAG
AGTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216527 representing NM_001161355
Red=Cloning site Green=Tags(s)

MNQIQVFISGLILLLPGAVESHTAVQGLAGHPVTLPCIYSTHLGGIVPMCWGLGECRHSYCIRSLIWTNG
YTVTHQRNSLYQLKGNISEGNVSLTIENTVVGDGGPYCCVVEIPGAFHFVDYMLEVKPEISTSPPTRPTA
TGRPTTISTRSTHVPTSTRVSTSTSPTPAHTETYKPEATTFYPDQTTAEVTETLPDTPADWHNTVTSSDD
PWDDNTEVIPPQKPQKNLNKGFYVGISIAALLILMLLSTMVITRYVVMKRKSESLSFVAFPISKIGASPK
KVVERTRCEDQVYIIEDTPYPEEES

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001161355
ORF Size 915 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001161355.1, NP_001154827.1
RefSeq Size 3153 bp
RefSeq ORF 918 bp
Locus ID 171284
UniProt ID Q8R183
Cytogenetics 11 B1.1
Summary Probable receptor for SEMA4A involved in the regulation of T-cell function. The interaction with SEMA4A enhances T-cell activation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001161355" in other vectors (3)
SKU Description Size Price
MR216527 Timd2 (Myc-DDK-tagged) - Mouse T-cell immunoglobulin and mucin domain containing 2 (Timd2), transcript variant 1 10 ug
$450.00
MR216527L3 Lenti ORF clone of Timd2 (Myc-DDK-tagged) - Mouse T-cell immunoglobulin and mucin domain containing 2 (Timd2), transcript variant 1 10 ug
$750.00
MR216527L4 Lenti ORF clone of Timd2 (mGFP-tagged) - Mouse T-cell immunoglobulin and mucin domain containing 2 (Timd2), transcript variant 1 10 ug
$750.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.