Spink8 (NM_183136) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG216406
Spink8 (tGFP-tagged) - Mouse serine peptidase inhibitor Kazal type 8 (Spink8), (10ug)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Spink8
Synonyms C630041L24Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216406 representing NM_183136
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGTGATCTTCTCAGTTGCAGTCCTTGTTCTGGCCAGCTCAGTGTGGACTTCCCTTGCAGTTGATT
TCATCCTTCCTATGAACTTTCACATGACCGGGGAGCTTCTACAAAAGACAAAGGCCTTGTGCATCAAGAA
TATACAGTTATGTTGGATACTTAGCTACTTCAAGGTCAGTGAGCCTATATGTGGCAGCAACCAAGTGACC
TACGAGGGCGAGTGCCATCTCTGCTCCGGAATTCTATATGAAGACAGAACTGTAATTAAAGTGCATGATG
GGCCATGTGAACACTCCTCGGATGAGTCTGAACAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216406 representing NM_183136
Red=Cloning site Green=Tags(s)

MKVIFSVAVLVLASSVWTSLAVDFILPMNFHMTGELLQKTKALCIKNIQLCWILSYFKVSEPICGSNQVT
YEGECHLCSGILYEDRTVIKVHDGPCEHSSDESEH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183136
ORF Size 315 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183136.2, NP_898959.2
RefSeq Size 692 bp
RefSeq ORF 318 bp
Locus ID 78709
UniProt ID Q09TK9
Cytogenetics 9 F2
Summary Probable serine protease inhibitor.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_183136" in other vectors (4)
SKU Description Size Price
MC211898 Spink8 (untagged) - Mouse serine peptidase inhibitor, Kazal type 8 (Spink8), (10ug) 10 ug
$165.00
MR216406 Spink8 (Myc-DDK-tagged) - Mouse serine peptidase inhibitor, Kazal type 8 (Spink8) 10 ug
$289.00
MR216406L3 Lenti ORF clone of Spink8 (Myc-DDK-tagged) - Mouse serine peptidase inhibitor, Kazal type 8 (Spink8) 10 ug
$450.00
MR216406L4 Lenti ORF clone of Spink8 (mGFP-tagged) - Mouse serine peptidase inhibitor, Kazal type 8 (Spink8) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.