OriGene Logo

Grsf1 (NM_178700) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG216357
Grsf1 (tGFP-tagged) - Mouse G-rich RNA sequence binding factor 1 (Grsf1) transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8
100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "Grsf1 or NM_178700"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Grsf1
Synonyms BB232551; C80280; D5Wsu31e
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216357 representing NM_178700
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGGCGCGCCC

ATGGCCGGGACGCGCTGGGTGCTAGGCGCGTTGCTCCGGGGCTGCGGCTGCAACTGCAGCAGCTGCCGGC
GGACCGGCGCCGCCTGCCTGCCTTTCTACTCCGCCGCCGGCACCTTCCCGTCGGGCGTCTCTGGCCGCCG
CCGCCTGCTGCTACTCCTCGGGGCCGCCGCGGCCGCCGCTTCGCAGACGCGGGGCCTGCAGCTCGGGCCT
GCGGCCGCCGGGAGGCTGGCGGGTCCCATCCCTGCCCGTCCCTCCGCCGCCGCGGCCGCCGCCGCGTCCT
ACTCGGCCCTGCGCGCCCCGCTGTTTCCGCGATCGCTGGCAGCCGCCGCGGGCCCGGCGCGGGGTTACAG
TCAGGAATCCAAAACTACCTACCTGGAAGACCTCCCACCACTGCCTGAGTACGAGTTGAGCCCATCCAAG
CTAGGAGACGAGGTGGATGATGTTTATCTCATTCGAGCTCAGGGGCTGCCGTGGTCCTGCACTGTGGAAG
ATGTTCTTAACTTTTTCTCAGACTGCAGAATCCGAAACAGTGAGAATGGAATACATTTCCTCCTAAATAG
AGATGGGAAACGGAGGGGCGATGCCTTAATAGAGATGGAGTCCGAGCAGGATGTACAGAAGGCCTTAGAA
AAGCACCGGATGTACATGGGGCAGCGGTATGTGGAAGTGTATGAAATAAACAACGAAGATGTGGATGCCT
TAATGAAGAGCCTGCAGGTCAAGCCTTCGCCTGTGCTCAGTGATGGTGTGGTCCGCCTGAGAGGACTTCC
TTACAGCTGCAATGAAAAGGACATTGTGGACTTCTTTGCAGGACTGAACATAGTAGACATTACTTTTGTG
ATGGACTATAGAGGGAGAAGAAAAACGGGGGAAGCCTATGTACAGTTTGAAGAACCAGAAATGGCCAACC
AAGCGCTTCTAAAGCACAGGGAAGAAATTGGTAACCGATACATAGAGATATTTCCAAGCAGAAGGAATGA
AGTTCGAACACATGTTGGATCTCATAAAGGAAAGAAAATGACATCGTCTCCTCCTACTAAATATATAACT
GAACCAGAAGTGGTATTTGAGGAACATGAAGTAAATGAAGATATTCGGCCTATGACGGCTTTTGAGAGTG
ATAAAGAGATAGAATTGCCTAAGGAGATGTCAGAAAAACTTCCAGAGGCTGTCGATTTTGGAACTCTGCC
TTCCCTGCATTTTGTCCACATGAGAGGATTGCCTTTCCAAGCCAATGCCCAAGACATTATAAATTTCTTT
GCTCCCCTGAAGCCTGTGAGAATCACCATGGAATACAGCTCCAGTGGGAAGGCCACTGGAGAAGCTGACG
TGCACTTTGATACCCATGAGGATGCTGTTGCAGCCATGCTCAAGGATCGGTCCCATGTCCAACATAGGTA
TATTGAGCTGTTCCTAAATTCATGTCCTAAAGGAAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216357 representing NM_178700
Red=Cloning site Green=Tags(s)

MAGTRWVLGALLRGCGCNCSSCRRTGAACLPFYSAAGTFPSGVSGRRRLLLLLGAAAAAASQTRGLQLGP
AAAGRLAGPIPARPSAAAAAAASYSALRAPLFPRSLAAAAGPARGYSQESKTTYLEDLPPLPEYELSPSK
LGDEVDDVYLIRAQGLPWSCTVEDVLNFFSDCRIRNSENGIHFLLNRDGKRRGDALIEMESEQDVQKALE
KHRMYMGQRYVEVYEINNEDVDALMKSLQVKPSPVLSDGVVRLRGLPYSCNEKDIVDFFAGLNIVDITFV
MDYRGRRKTGEAYVQFEEPEMANQALLKHREEIGNRYIEIFPSRRNEVRTHVGSHKGKKMTSSPPTKYIT
EPEVVFEEHEVNEDIRPMTAFESDKEIELPKEMSEKLPEAVDFGTLPSLHFVHMRGLPFQANAQDIINFF
APLKPVRITMEYSSSGKATGEADVHFDTHEDAVAAMLKDRSHVQHRYIELFLNSCPKGK

TRTRPLE - GFP Tag - V
Restriction Sites AscI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178700
ORF Size 1437 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178700.5, NP_848815.2
RefSeq Size 2749 bp
RefSeq ORF 1440 bp
Locus ID 231413
UniProt ID Q8C5Q4
Cytogenetics 5 43.85 cM
Summary Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_178700" in other vectors (4)
SKU Description Size Price
MC216720 Grsf1 (untagged) - Mouse G-rich RNA sequence binding factor 1 (Grsf1), transcript variant 1, (10ug) 10 ug
$503.00
MR216357 Grsf1 (Myc-DDK-tagged) - Mouse G-rich RNA sequence binding factor 1 (Grsf1), transcript variant 1 10 ug
$289.00 $457.00
MR216357L3 Lenti ORF clone of Grsf1 (Myc-DDK-tagged) - Mouse G-rich RNA sequence binding factor 1 (Grsf1), transcript variant 1 10 ug
$757.00
MR216357L4 Lenti ORF clone of Grsf1 (mGFP-tagged) - Mouse G-rich RNA sequence binding factor 1 (Grsf1), transcript variant 1 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.