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Fbxl22 (NM_175206) Mouse Tagged ORF Clone

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SKU
MG216331
Fbxl22 (tGFP-tagged) - Mouse F-box and leucine-rich repeat protein 22 (Fbxl22), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Fbxl22 or NM_175206"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fbxl22
Synonyms 1110004B15Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216331 representing NM_175206
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACATAACCCAGCTCAATCGGGAGTGTCTGCTGTGTCTCTTCTCCTTCCTGGACAAGGACAGCCGGA
GGAGCCTCTCCAGGACTTGCTCCCAGCTCCGAGATGTGTTTGAGGACCCCACCCTCTGGCCCCTGCTGCA
CTTCCATTCTCTCGCAGAGCTCAAGAAAGACAACTTCCGGCTGAGCCCTGCGCTGCGCAGCCTGTCCATC
TGTTGGCATTCCAGCCGTGTGCAGGTGTGCAGCATCGAGGACTGGCTCAAGAGCGCCCTCCAGAGGAGCA
TCTGCAGCCAGCACGAGAGCCTGGTTAATGATTTCCTCCTGCAGGTGTGCAACAGGTGCCCCAACCTGAC
GTCCGTCACGCTGTCGGGCTGCGGCCACGTCACGGACGACTGCCTGGCGCGCTTGCTGCTCAGCTGCCCG
CGCCTGCGTACGCTGCGCCTCGAGAACTGCGCGCGCGTTACCAACCGCACGCTGGCGGCCGTGGCCGCGC
ACGGGCGCGCGCTGCAGACTCTGCACGTGGACTTCTGCCGCAACGTGAGCGCCGCCGGCCTGCTCCGCCT
CCGCGCCGCCTGCCCGAACCTGCGCCTCAGCGCCGAACGCAGCGCGGCCATGATACCCGATCAGCCGCCG
CGCGCACGCGTGTGCGCTGCCTCAGCTTTCTCGGCTGCACCGGGGAGACAAAGCATGCCTCACCCCACGG
GCTCCTAC

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216331 representing NM_175206
Red=Cloning site Green=Tags(s)

MHITQLNRECLLCLFSFLDKDSRRSLSRTCSQLRDVFEDPTLWPLLHFHSLAELKKDNFRLSPALRSLSI
CWHSSRVQVCSIEDWLKSALQRSICSQHESLVNDFLLQVCNRCPNLTSVTLSGCGHVTDDCLARLLLSCP
RLRTLRLENCARVTNRTLAAVAAHGRALQTLHVDFCRNVSAAGLLRLRAACPNLRLSAERSAAMIPDQPP
RARVCAASAFSAAPGRQSMPHPTGSY

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_175206
ORF Size 708 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175206.4, NP_780415.1
RefSeq Size 3935 bp
RefSeq ORF 711 bp
Locus ID 74165
UniProt ID Q8C7B6
Cytogenetics 9 C
Summary Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Promotes ubiquitination of sarcomeric proteins alpha-actinin-2 (ACTN2) and filamin-C (FLNC) (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175206" in other vectors (4)
SKU Description Size Price
MC211492 Fbxl22 (untagged) - Mouse F-box and leucine-rich repeat protein 22 (Fbxl22), (10ug) 10 ug
$330.00
MR216331 Fbxl22 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 22 (Fbxl22) 10 ug
$289.00 $300.00
MR216331L3 Lenti ORF clone of Fbxl22 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 22 (Fbxl22) 10 ug
$600.00
MR216331L4 Lenti ORF clone of Fbxl22 (mGFP-tagged) - Mouse F-box and leucine-rich repeat protein 22 (Fbxl22) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.