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Rnase2b (NM_019398) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216330
Ear5 (tGFP-tagged) - Mouse eosinophil-associated ribonuclease A family member 5 (Ear5), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rnase2b
Synonyms Ear5; mR5; Raf4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216330 representing NM_019398
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTCTGAAGCTGCTTGAATCCAGACTTTGTCTCCTGCTGCTGCTGGGACTTGTCCTAACGCTTGTCT
CATGCCAGCGACCAACCCCTTCCCAGAAGTTTGACATCCAGCATATCTATAAGAAATCCTCTCCCAAATG
TGATGATGCAATGCGGGTCGTTAACAAGTATACAGGAAAATGTAAGGACTTGAATACTTTTCTTCATACA
ACTTTTGCTGATGTTGTCCGTGTGTGTCACAATCCACCCAAGACTTGCAAAGACGGGACAAGTCCAAATT
GTCATGATAGTTCATCTAAGGTATCTGTAACTATCTGTAAACTCACAAAACGGGCAAGGAATTATACCCA
CTGCAGATACAAAACAACAGGAGCAAAGAAGTCCTACACAGTTGCCTGTAACCCCAGAACTCCAAAGGAC
CGTCCCACCTATCCAGTAGTTCCGGTTCACTTGGATCGGCTATTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216330 representing NM_019398
Red=Cloning site Green=Tags(s)

MGLKLLESRLCLLLLLGLVLTLVSCQRPTPSQKFDIQHIYKKSSPKCDDAMRVVNKYTGKCKDLNTFLHT
TFADVVRVCHNPPKTCKDGTSPNCHDSSSKVSVTICKLTKRARNYTHCRYKTTGAKKSYTVACNPRTPKD
RPTYPVVPVHLDRLF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019398
ORF Size 465 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019398.2, NP_062271.2
RefSeq Size 759 bp
RefSeq ORF 468 bp
Locus ID 54159
UniProt ID O35292
Cytogenetics 14 C1
Summary This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Possesses a wide variety of biological activities.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019398" in other vectors (4)
SKU Description Size Price
MC209955 Ear5 (untagged) - Mouse eosinophil-associated, ribonuclease A family, member 5 (Ear5), (10ug) 10 ug
$165.00
MR216330 Ear5 (Myc-DDK-tagged) - Mouse eosinophil-associated, ribonuclease A family, member 5 (Ear5) 10 ug
$289.00
MR216330L3 Lenti ORF clone of Ear5 (Myc-DDK-tagged) - Mouse eosinophil-associated, ribonuclease A family, member 5 (Ear5) 10 ug
$450.00
MR216330L4 Lenti ORF clone of Ear5 (mGFP-tagged) - Mouse eosinophil-associated, ribonuclease A family, member 5 (Ear5) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.