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Sash1 (NM_175155) Mouse Tagged ORF Clone

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SKU
MG216244
Sash1 (tGFP-tagged) - Mouse SAM and SH3 domain containing 1 (Sash1), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,271.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Sash1 or NM_175155"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sash1
Synonyms 1100001C18Rik; 2500002E12Rik; A330076K04Rik; mKIAA0790
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216244 representing NM_175155
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGGACGCGGGAGCTGCTAGCCCGGCGCCGGAGCCTGAGCCGGAGGTGGATCCCGCCCGGGAGC
TGGAGCCCGAGGCCGGTGTCTCCGAGTCAATCTCCCGCCTCTGGACCGACGTGATGGGCATCCTGGATGG
TTCCCTGGGAAACATTGATGACCTGGCACAGCAGTATGCAGATTATTACAATACCTGCTTTTCTGACGTG
TGTGAGCGGATGGAGGAGCTCCGGAAACGGCGCGTTTCCCAGGACCTGGATGTGGAGAAACCCGACGCCA
GCCCCACGTCACTTCAGCTGCGGTCCCAGATCGAAGAGTCTCTAGGCTTCTGCAGCGCCGTGTCAACACC
AGAAGTGGAAAGAAAGTACCCTCTCCATAAATCAAACTCAGAAGACGGCTGTGTAGGCAAAGGAGATTGG
AAGAAGAAAAATAAATACTTCTGGCAGAATTTCCGAAAGAACCAAAAAGGAATAATGAGACAGACTTCAA
AAGGAGAAGATGTGGGCTATGTAGCCAGCGAGATTACGATGAGTGACGAGGAGCGGATTCAGCTGATGAT
GATGGTCAAGGAGAAAATGATCACCATTGAAGAAGCGCTCGCCAGGCTGAAGGAATACGAGGCCCAGCAC
CGTCAGTCATCCACCTTGGACCCCGCAGACTGGCCCGATGGCTCCTACCCGACATTGGATGGCTCCTCCA
CCTGCAACTCAAGAGAGCAGTCGGATGATGAGACAGAGGACTCGGTGAAGTTTAAGAGGTTGCACAAGCT
GGTAAACTCTACTCGAAGAGTCAGAAAGAAACTAATTAGGGTGGAAGAAATGAAAAAGCCCAGCACAGAA
GGTGGAGAGGAACATGTATTTGAGAATTCCCCAGTCCAAGATGAAAGATCTGCACTCTACTCTGGAGTGC
ACAAAAAGCCATTTTTCTATGATGGCTCTCCTGAGAAACCTCCAGAAGATGATGCAGACTCTCTCACCCC
ATCTCCATCATCCAGCAGCCTGGATACGTGGGGAGCTGGCCGGAAGTTGGTCAAAACCTTCAGCAAAGGA
GAGAGTCGGGGCCTGATTAAGCCCCCTAAGAAGATGGGGACATTCTTCTCCTATCCAGAAGAAGAGAAGG
CCCAGAAAGTGTCCCGCTCCCTCACAGAGGGGGAAATGAAGAAAGGTCTTGGGTCTTTGAGTCACGGGAG
AACCTGCAGTTTTGGTGGATTCGACCTGACAAACCGTTCTCTGCATGTTGGCAGTAATAATTCTGACCCT
GCGGGTAAAGAGGGAGATTTTGTGTACAAAGAAGTCATCAAATCCCCTCCTGCCCCCCGCATCTCTCTCG
GGAAAAAGGTGAGATCAGTGAAAGAGACCATGAGGAAGAGGATGTCTAAAAAGTACAGCAGCCCGGTCTC
AGAGCAGGACTCGGGGCTCGATGGAATGCCCAGCTCCCCCGCCTCAGGAAAGCCTGACTCCGAACACGTG
GACAAGCCTAAGCTCAAAGCCGGGGGCTCTGTGGAAAGCCTTCGGAGTTCTCTGAGTGGCCAGAGCTCAA
TGAGTGGTCAGACAGTGAGCACCACTGATTCCTCCACCAGCAACCGGGAGAGTGTCAAGTCAGAAGATGG
GGATGACGAAGAGCCACCCTATCGGGGTCCCTTCTGTGGGCGTGCCCGAGTGCACACTGACTTCACCCCC
AGTCCCTATGACACGGACTCTCTGAAGCTCAAGAAAGGAGATATCATTGACATAATTAGCAAACCACCCA
TGGGTACCTGGATGGGCCTGCTGAACAACAAAGTCGGCACGTTCAAGTTCATCTACGTGGATGTGCTAAA
TGAGGAAGAGGAGAAGCCCAAGCGCCCTACCAGGAGGAGGAAGAAGGGAAGACCATCTCAGCCGAAGTCT
GTGGAGGATCTCCTGGACCGGATCAACCTGAAAGAACACATGCCCACCTTCCTGTTCAATGGCTATGAAG
ACCTGGACACCTTCAAACTCCTGGAGGAAGAAGACTTAGATGAATTAAATATCAGGGACCCAGAACACAG
AGCAGTTCTTCTGACTGCGGTGGAGCTGTTGCAGGAATATGACAGTAACAGTGACCAGTCCGGTTCTCAG
GAAAAGCTGCTGGTGGACAACCAGGGCCTGAGTGGACGATCCCCACGAGACTCCGGATGCTACGAAAGCA
GCGAGAACTTGGAAAACGCCAAAACTCACAAACCCAGCGTTCTGTCTACCAAGTCGTCCACGGAGTCCAA
CTTAAAGTCTTTCACCAGGAGTCAGCCAGGCAACTACCCGACATTGCCTTTAATGAAATCAGGGGAGGTG
CGCAAGCAGGGAGAGGAGGGCAGGCTGGGACGAGGTCTAGCCCCGGACACTGCCAAGAGCTGTGACGTGC
CCTCTGTGACTGATTTGAGTAAAAACCGAAGGAGCCTCCCGGTCTCCATCTGTCGCAGCTGTGAAACCCT
GGAGGGTCCTGAGCCTGTGGAAAGTTGGCCCCGATCCCATTCCCTGGATGACCTTCAGGGAGATGCTGAT
GTTGGCAAGAATGTGCCTACCGAGATGCCAGAAACCTGTTCTCAGAATGTACCAGAAGTGCCGCAGAAGA
CGTCTGCCTGCACCTCCAAGGCTCTGCCACGAGGCCGAGATCCCACAGCAGATGTGATGCTTCTGACCCA
AAGCAAGAGATTTTCCGACCCCCCGAAAACAATGGCTAAGAAACTGGATGGCTCCGTGGTGGCCTCCAAC
CTTGGGATAGCTCCTCCTCAGTGTATACCCAGAGATTTCGAGGCCCAGCCGCCTGTTAAACCCGGCTTAA
CAAGGACGTCTCTTGAGGGTCTCAGAAAAGGACATGATCACCACCCCCTGGGCACCAAAGAAGGGGTAGA
CGGGGAGCAGAGCGCCCCTGAGACCAGGACACAGTCCCGACATCCGTCGCAGCCCCCTCCTGTGCCAGCC
AAGAAAAGCCGAGAGCGCCTGGCCAATGGCCTGCACTTGGTCCCCAGCCCAGAGGCACCCATCCTGCCAC
TGAAGAAAGCCAGCCCTGCCAGCCCAGTCAGTCCCAGCGACTGCCCCTCTCCCCGGGAACCCAGGCCTTC
CTCCGGGACTGAGCCGGGCAGCCCAGCCTGTACGAGACCTCCACCCTGGCTGGCTGAGCTGCCCGAGAGC
ACTAGCCTGCAGGAGCATGGTGTGAAACTGGGCCCAGTGCTGAGCAGGAAGGTTTCCTGTGTCCGGGGTG
TGGACCTAGAGATGCTCACGGAGAACAAGCTGCAGGCGGAGGGCATTGATCTCACTGAGGAGCCCTACTC
TGATAAGCATGGCCGTTGTGGGATCCCGGAAGCCCTGGTACAGAGATACGCAGAGGACCTAGAGCAGCCT
GAGAGGGATGTTGCCACCAACATGGACCAGATCCGGGTGAAGCTGCTTCGGAAGCAACATCGGATGGCGA
TCCCGAGTGGTGGGCTGACGGAAATCTGCAGAAAGCCCCTCTCTCCCGGCTGTGTTGCGTCCATGTCGGA
TTGGCTCATCTCTATCGGCCTGCCCATGTACACGAGCACCCTTTCTGACGCAGGGTTCAGCACGCTGAGC
CAAGTGCCTTCCCTGTCCCACTCTTGCCTTCAGGAGGCCGGCATCACCGAGGAGAGACACATAAGGAAAC
TCATAACGGCAGCCAGACTCTTCAAACTGCCACCAAGCCCTGAGGCTATG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216244 representing NM_175155
Red=Cloning site Green=Tags(s)

MEEDAGAASPAPEPEPEVDPARELEPEAGVSESISRLWTDVMGILDGSLGNIDDLAQQYADYYNTCFSDV
CERMEELRKRRVSQDLDVEKPDASPTSLQLRSQIEESLGFCSAVSTPEVERKYPLHKSNSEDGCVGKGDW
KKKNKYFWQNFRKNQKGIMRQTSKGEDVGYVASEITMSDEERIQLMMMVKEKMITIEEALARLKEYEAQH
RQSSTLDPADWPDGSYPTLDGSSTCNSREQSDDETEDSVKFKRLHKLVNSTRRVRKKLIRVEEMKKPSTE
GGEEHVFENSPVQDERSALYSGVHKKPFFYDGSPEKPPEDDADSLTPSPSSSSLDTWGAGRKLVKTFSKG
ESRGLIKPPKKMGTFFSYPEEEKAQKVSRSLTEGEMKKGLGSLSHGRTCSFGGFDLTNRSLHVGSNNSDP
AGKEGDFVYKEVIKSPPAPRISLGKKVRSVKETMRKRMSKKYSSPVSEQDSGLDGMPSSPASGKPDSEHV
DKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSTSNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTP
SPYDTDSLKLKKGDIIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLNEEEEKPKRPTRRRKKGRPSQPKS
VEDLLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIRDPEHRAVLLTAVELLQEYDSNSDQSGSQ
EKLLVDNQGLSGRSPRDSGCYESSENLENAKTHKPSVLSTKSSTESNLKSFTRSQPGNYPTLPLMKSGEV
RKQGEEGRLGRGLAPDTAKSCDVPSVTDLSKNRRSLPVSICRSCETLEGPEPVESWPRSHSLDDLQGDAD
VGKNVPTEMPETCSQNVPEVPQKTSACTSKALPRGRDPTADVMLLTQSKRFSDPPKTMAKKLDGSVVASN
LGIAPPQCIPRDFEAQPPVKPGLTRTSLEGLRKGHDHHPLGTKEGVDGEQSAPETRTQSRHPSQPPPVPA
KKSRERLANGLHLVPSPEAPILPLKKASPASPVSPSDCPSPREPRPSSGTEPGSPACTRPPPWLAELPES
TSLQEHGVKLGPVLSRKVSCVRGVDLEMLTENKLQAEGIDLTEEPYSDKHGRCGIPEALVQRYAEDLEQP
ERDVATNMDQIRVKLLRKQHRMAIPSGGLTEICRKPLSPGCVASMSDWLISIGLPMYTSTLSDAGFSTLS
QVPSLSHSCLQEAGITEERHIRKLITAARLFKLPPSPEAM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175155
ORF Size 3690 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175155.4, NP_780364.3
RefSeq Size 7183 bp
RefSeq ORF 3693 bp
Locus ID 70097
UniProt ID P59808
Cytogenetics 10 A1
Summary May have a role in a signaling pathway. Could act as a tumor suppressor.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175155" in other vectors (4)
SKU Description Size Price
MC224014 Sash1 (untagged) - Mouse SAM and SH3 domain containing 1 (Sash1), (10ug) 10 ug
$1,179.00
MR216244 Sash1 (Myc-DDK-tagged) - Mouse SAM and SH3 domain containing 1 (Sash1) 10 ug
$589.00 $1,071.00
MR216244L3 Lenti ORF clone of Sash1 (Myc-DDK-tagged) - Mouse SAM and SH3 domain containing 1 (Sash1) 10 ug
$1,371.00
MR216244L4 Lenti ORF clone of Sash1 (mGFP-tagged) - Mouse SAM and SH3 domain containing 1 (Sash1) 10 ug
$1,371.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.