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Eml2 (NM_028153) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216243
Eml2 (tGFP-tagged) - Mouse echinoderm microtubule associated protein like 2 (Eml2) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $812.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Eml2
Synonyms 1600029N02Rik; EMAP-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216243 representing NM_028153
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTAGTTTTGGAATTGGGAAAACCAAAGAAGTTATCTTCAGCATGGAGGAGGGATCCGTAAAGATGT
TTCTGCGGGGACGCCCTGTGCCCATGCTGATCCCGGACGAACTGGCGCCCACCTATAGCCTGGACACTCG
TTCTGAGCTGCCCTCCAGCCGGCTAAAGCTAGACTGGGTGTATCCTTTGACTAGCTCCCTTTCTGCCCTC
TTGGAAGAGGCCCTGGGCTTCTCCAGCTATGGCTATCGAGGCCGGGACTGCAGGGCCAACCTTTACCTCC
TGCCCACTGGGGAGGTGGTGTACTTTGTGGCCTCTGTGGCCGTACTGTACAGCGTGGAGGAGCAGAGACA
ACGACACTACCTGGGGCACAACGATGACATCAAATGTCTGGCTGTCCATCCAGATATGGTCACCATCGCC
ACAGGACAGGTGGCAGGCACCACAAAGGAAGGCAAGCCTCTGCCACCCCATGTGCGTGTGTGGGACTCGG
TTTCCCTTTCCACCTTACACGTCCTGGGCCTGGGGGTGTTTGACAGAGCTGTGTGCTGTGTGGCCTTCTC
CAAATCCAATGGGGGCAACCTGCTATGTGCAGTGGACGAATCCAACGACCATGTGCTCTCTGTGTGGGAC
TGGGCCAAGGAGAGCAAAGTGGTGGATAGCAAGTGCTCTAACGAGGCAGTGCTAGTGGCCACCTTCCACC
CCACTGATCCCAGCCTGCTGATAACCTGTGGCAAATCCCACATCTACTTCTGGAGCCTGGAGGGAGGCAG
TCTGAGCAAGAGGCAGGGCCTGTTCGAGAAACACGAGAAACCAAAGTACGTGCTGTGTGTGACCTTCCTG
GAAGGTGGCGATGTGGTCACTGGAGACTCTGGGGGGAACCTCTATGTCTGGGGCAAAGGTGGAAACCGCA
TCACCCAGGAAGTTCAAGGAGCCCATGATGGTGGCGTGTTTGCTCTCTGCGCCCTGCGGGACGGGACGCT
GGTGTCTGGAGGGGGCCGCGATCGTAGGGTGGTCCTCTGGGGTTCGGACTACAGCAAGGTGCAGGAGGTG
GAGGTCCCAGAGGACTTCGGCCCTGTACGCACCGTGGCAGAGGGCCGGGGAGACACACTGTACGTGGGGA
CCACCCGTAACTCCATCTTGCTAGGCTCGGTGCACACAGGGTTTTCGCTGCTTGTCCAGGGACATGTGGA
GGAGCTGTGGGGCCTGGCCACACATCCTAGCAGGGCACAGTTTGTGACCTGTGGGCAGGACAAGCTGGTG
CATCTGTGGAGTTCCGAGACCCACCAGCCAGTGTGGAGCAGGAGCATCGAGGACCCTGCCCGTTCGGCTG
GCTTCCACCCCAGTGGCTCCGTCCTAGCGGTGGGCACAGTGACCGGCAGGTGGCTGCTACTGGACACAGA
GACCCATGACCTGGTGGCTATCCACACAGATGGCAATGAGCAGATCTCCGTGGTCAGCTTTTCACCAGAC
GGGGCGTACCTGGCTGTGGGCTCCCACGACAACTTGGTGTACGTGTACACGGTGGACCAGGGTGGCCGCA
AGGTCAGCCGCCTGGGCAAGTGCTCGGGCCATTCCAGTTTTATCACCCACCTGGATTGGGCCCAGGACAG
CACCTGCTTTGTCACCAACTCTGGAGACTACGAGATTCTGTACTGGGACCCCGTCACCTGTAAGCAAATC
ACCAGTGCAGACACCGTGAGAAACGTGGAATGGGCTACCGCCACTTGTGTGTTGGGTTTTGGAGTGTTTG
GGATTTGGCCCGAGGGAGCAGATGGCACAGACATCAATGCTGTGGCCCGCTCTCATGATGGGAAGTTGCT
GGTTTCTGCTGACGACTTTGGCAAAGTGCACCTGTTCAGCTACCCCTGCTGTCAGCCGAGAGCCCTCAGC
CACAAATACGGTGGACACAGCAGCCATGTGACTAATGTGGCCTTCTTGTGGGATGACAGCATGGCCCTAA
CCACTGGGGGCAAAGACACGAGCGTGCTGCAGTGGCGGGTGGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216243 representing NM_028153
Red=Cloning site Green=Tags(s)

MSSFGIGKTKEVIFSMEEGSVKMFLRGRPVPMLIPDELAPTYSLDTRSELPSSRLKLDWVYPLTSSLSAL
LEEALGFSSYGYRGRDCRANLYLLPTGEVVYFVASVAVLYSVEEQRQRHYLGHNDDIKCLAVHPDMVTIA
TGQVAGTTKEGKPLPPHVRVWDSVSLSTLHVLGLGVFDRAVCCVAFSKSNGGNLLCAVDESNDHVLSVWD
WAKESKVVDSKCSNEAVLVATFHPTDPSLLITCGKSHIYFWSLEGGSLSKRQGLFEKHEKPKYVLCVTFL
EGGDVVTGDSGGNLYVWGKGGNRITQEVQGAHDGGVFALCALRDGTLVSGGGRDRRVVLWGSDYSKVQEV
EVPEDFGPVRTVAEGRGDTLYVGTTRNSILLGSVHTGFSLLVQGHVEELWGLATHPSRAQFVTCGQDKLV
HLWSSETHQPVWSRSIEDPARSAGFHPSGSVLAVGTVTGRWLLLDTETHDLVAIHTDGNEQISVVSFSPD
GAYLAVGSHDNLVYVYTVDQGGRKVSRLGKCSGHSSFITHLDWAQDSTCFVTNSGDYEILYWDPVTCKQI
TSADTVRNVEWATATCVLGFGVFGIWPEGADGTDINAVARSHDGKLLVSADDFGKVHLFSYPCCQPRALS
HKYGGHSSHVTNVAFLWDDSMALTTGGKDTSVLQWRVA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028153
ORF Size 2004 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028153.1, NP_082429.1
RefSeq Size 2312 bp
RefSeq ORF 2007 bp
Locus ID 72205
UniProt ID Q7TNG5
Cytogenetics 7 A3
Summary Tubulin binding protein that inhibits microtubule nucleation and growth, resulting in shorter microtubules.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028153" in other vectors (4)
SKU Description Size Price
MC220265 Eml2 (untagged) - Mouse echinoderm microtubule associated protein like 2 (Eml2), transcript variant 1, (10ug) 10 ug
$674.00
MR216243 Eml2 (Myc-DDK-tagged) - Mouse echinoderm microtubule associated protein like 2 (Eml2), transcript variant 1 10 ug
$589.00 $612.00
MR216243L3 Lenti ORF clone of Eml2 (Myc-DDK-tagged) - Mouse echinoderm microtubule associated protein like 2 (Eml2), transcript variant 1 10 ug
$912.00
MR216243L4 Lenti ORF clone of Eml2 (mGFP-tagged) - Mouse echinoderm microtubule associated protein like 2 (Eml2), transcript variant 1 10 ug
$912.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.