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Fbxl20 (NM_028149) Mouse Tagged ORF Clone

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SKU
MG216205
Fbxl20 (tGFP-tagged) - Mouse F-box and leucine-rich repeat protein 20 (Fbxl20), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fbxl20
Synonyms 2610511F20Rik; 4632423N09Rik; AI849362; AL117906; C86145; Fbl2; mKIAA4147; Scr
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216205 representing NM_028149
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAGGGACGTGAACGGAGTGACCAAGAGCAGGTTTGAGATGTTCTCAAACAGTGATGAGGCTGTAA
TCAATAAAAAACTTCCCAAAGAACTCCTGTTGAGGATATTTTCTTTCCTGGATGTTGTTACCCTGTGTCG
CTGTGCTCAGGTCTCCAGGGCCTGGAATGTCCTAGCTCTGGATGGCAGTAACTGGCAACGGATAGACCTG
TTTGATTTCCAGAGGGACATTGAGGGCCGGGTAGTGGAAAATATTTCAAAACGATGTGGAGGCTTTTTAC
GAAAGTTAAGTCTTCGTGGGTGTCTTGGAGTAGGAGACAATGCATTAAGGACCTTTGCACAAAACTGTAG
GAATATTGAAGTACTAAGTCTCAACGGATGTACAAAGACAACAGATGCTACCTGCACTAGCCTTAGCAAG
TTCTGCTCCAAACTCAGACACCTCGACTTGGCTTCCTGTACGTCAATAACAAACATGTCTCTAAAAGCTC
TGAGTGAGGGCTGTCCACTGTTGGAGCAGTTGAACATTTCCTGGTGTGACCAAGTAACCAAGGATGGCAT
TCAAGCACTAGTGAGAGGCTGTGGGGGTCTCAAGGCCTTATTCTTAAAAGGCTGCACTCAGCTAGAAGAT
GAAGCTCTCAAGTACATAGGTGCACACTGCCCTGAGCTAGTGACTTTAAACCTGCAAACTTGCTTACAAA
TCACAGATGAAGGTCTCATTACCATATGCAGAGGGTGTCATAAGTTGCAGTCCCTCTGTGCCTCTGGGTG
CTCCAATATCACAGATGCCATCCTGAACGCTCTAGGTCAGAACTGCCCTCGACTTAGAATATTAGAAGTG
GCCAGATGTTCCCAGTTAACAGATGTGGGCTTCACTACCCTAGCCAGGAATTGCCATGAGCTTGAGAAGA
TGGACCTAGAAGAATGTGTGCAGATCACGGATAGCACATTAATCCAGCTTTCCATTCACTGTCCTCGGCT
TCAAGTGTTGAGTCTTTCTCACTGTGAGCTGATCACAGACGATGGAATCCGTCACCTAGGGAACGGGGCC
TGTGCCCATGACCAGCTGGAGGTGATAGAGCTGGACAACTGCCCACTAATCACCGATGCATCCCTGGAGC
ACTTGAAGAGCTGTCACAGCCTTGAGCGAATAGAACTCTATGACTGCCAGCAGATCACCCGGGCTGGCAT
CAAGAGACTCAGGACCCATTTACCCAACATTAAAGTCCACGCCTACTTCGCCCCTGTCACTCCGCCCCCA
TCAGTCGGGGGCAGCAGACAGCGCTTCTGCAGGTGCTGCATCATCCTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216205 representing NM_028149
Red=Cloning site Green=Tags(s)

MRRDVNGVTKSRFEMFSNSDEAVINKKLPKELLLRIFSFLDVVTLCRCAQVSRAWNVLALDGSNWQRIDL
FDFQRDIEGRVVENISKRCGGFLRKLSLRGCLGVGDNALRTFAQNCRNIEVLSLNGCTKTTDATCTSLSK
FCSKLRHLDLASCTSITNMSLKALSEGCPLLEQLNISWCDQVTKDGIQALVRGCGGLKALFLKGCTQLED
EALKYIGAHCPELVTLNLQTCLQITDEGLITICRGCHKLQSLCASGCSNITDAILNALGQNCPRLRILEV
ARCSQLTDVGFTTLARNCHELEKMDLEECVQITDSTLIQLSIHCPRLQVLSLSHCELITDDGIRHLGNGA
CAHDQLEVIELDNCPLITDASLEHLKSCHSLERIELYDCQQITRAGIKRLRTHLPNIKVHAYFAPVTPPP
SVGGSRQRFCRCCIIL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028149
ORF Size 1308 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028149.1, NP_082425.1
RefSeq Size 8607 bp
RefSeq ORF 1311 bp
Locus ID 72194
UniProt ID Q9CZV8
Cytogenetics 11 D
Summary Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Isoform 3 regulates neural transmission by binding and ubiquitinating RIMS1, a modulator of presynaptic plasticity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028149" in other vectors (4)
SKU Description Size Price
MC215734 Fbxl20 (untagged) - Mouse F-box and leucine-rich repeat protein 20 (Fbxl20), (10ug) 10 ug
$503.00
MR216205 Fbxl20 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 20 (Fbxl20) 10 ug
$289.00 $457.00
MR216205L3 Lenti ORF clone of Fbxl20 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 20 (Fbxl20) 10 ug
$757.00
MR216205L4 Lenti ORF clone of Fbxl20 (mGFP-tagged) - Mouse F-box and leucine-rich repeat protein 20 (Fbxl20) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.