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Sema5b (NM_013661) Mouse Tagged ORF Clone

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SKU
MG216182
Sema5b (tGFP-tagged) - Mouse sema domain seven thrombospondin repeats (type 1 and type 1-like) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 5B (Sema5b), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,151.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sema5b
Synonyms AI893641; mKIAA1445; Semag; SemG
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216182 representing NM_013661
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTAGTCCCAGGACCCCTGGCTCTCTCGCTGTTGCTGTCCAGCCTCACCCTGCTGGTGTCCCACCTCT
CCAGCTCCCAGGACATTGCCAGTGAGTCCAGCAGTGAGCAACAGATGTGCACGCGGAGGGAGCACCCCAT
CGTGGCCTTTGAAGATCTGAAGCCGTGGGTCTTTAACTTCACCTACCCTGGAGTCCGGGATTTCTCCCAG
CTTGCTCTTGATCCCTCGAGGAATCAGCTCATCGTGGGAGCCAGGAACTACCTCTTCAGACTCAGCCTCG
CCAATGTCTCCCTCCTTCAGGCTACAGAGTGGGCCTCCAGTGAGGACACGCGCCGCTCCTGCCAGAGCAA
AGGGAAAACGGAGGAGGAGTGTCAGAACTATGTACGAGTCCTGATTGTTTCCGGCCGGAAGGTGTTCATG
TGCGGTACCAATGCCTTTTCCCCAGTGTGCTCCAGCAGACAGGTAGGGAACCTCAGCCGGACTATTGAGA
AGATCAACGGTGTGGCCCGGTGCCCCTATGACCCACGCCACAACTCTACAGCCGTCATCTCCTCTCAGGG
GGAGCTCTATGCAGCCACAGTCATTGACTTCTCCGGTCGGGACCCAGCCATCTACCGCAGCCTGGGCAGT
GGGCCACCGCTTCGCACTGCCCAGTATAACTCCAAATGGCTCAATGAGCCAAATTTTGTGGCAGCCTTTG
ACATCGGGCTGTTTGCGTATTTCTTCCTTCGGGAGAATGCCGTGGAGCATGATTGTGGGCGCACTGTGTA
CTCTCGGGTGGCCCGGGTGTGCAAGAATGATGTAGGTGGCCGTTTCCTGCTGGAGGACACGTGGACCACA
TTCATGAAGGCCCGGCTCAACTGCTCCCGCCCGGGAGAGGTCCCCTTCTACTATAATGAGCTGCAGAGTG
CCTTCCATCTGCCCGAGCAGGACCTCATCTATGGCGTCTTCACCACTAACGTAAACAGCATTGCGGCTTC
TGCTGTCTGCGCCTTCAACCTCAGTGCCATCTCCAAGGCTTTCAATGGCCCATTTCGTTACCAGGAAAAC
CCCAGGGCTGCCTGGCTCCCAATCGCCAATCCCATCCCCAATTTCCAGTGTGGCACTCTGCCGGAGACTG
GCCCCAACGAGAACCTCACGGAGCGCAGCCTGCAGGACGCACAGCGGCTCTTCCTGATGAGCGAAGCTGT
GCAGCCAGTGACACCAGAGCCCTGTGTCACCCAGGACAGCGTCCGCTTCTCACATCTCGTGGTAGACCTT
GTGCAAGCTAAGGACACGCTCTATCACGTACTCTACATAGGCACGGAGTCGGGTACCATCCTGAAAGCGC
TGTCCACGGCCAGCCGCAGCCTCCGTGGCTGCTACCTGGAGGAGCTACATGTGCTGCCTCCTGGGCGCCT
TGAACCTCTGCGGAGCCTGCGCATCCTGCACAGCGCGCGTGCGCTCTTCGTGGGATTGAGCGACAGGGTG
CTGCGGGTCCCACTGGAGAGGTGTTCGGCCTATCATAGCCAGGGGGCATGCCTGGGAGCACGGGACCCAT
ACTGCGGCTGGGATGGGAAGCGGCAACTTTGCAGCACGCTTGAAGACAGTTCCAACATGAGCCTGTGGAT
CCAGAACATCACAACCTGTCCTGTACGAAATGTGACACGGGATGGGGGCTTCGGCCCATGGTCACCATGG
AAACCGTGTGAGCACTTAGATGGAGACAACTCGGGTTCTTGCCTGTGCCGGGCCAGATCCTGTGACTCCC
CAAGGCCTCGCTGTGGGGGCCTCGAGTGCCTGGGGCCATCCATCCATATTGCCAATTGCTCCAGGAATGG
GGCGTGGACCGCATGGTCATCGTGGGCTCAGTGCAGCACGTCCTGTGGGATCGGCTTCCAGGTCCGTCAG
CGAAGTTGCAGCAACCCGGCGCCCCGCCATGGGGGCCGCATTTGCGTGGGCAAGAGCCGGGAGGAGCGGT
TCTGTAATGAAAATACACCTTGCCCAGTGCCCATCTTCTGGGCTTCCTGGGGTTCCTGGAGCAAGTGCAG
CAACAACTGTGGAGGCGGCGTGCAGTCGCGACGCCGTTCTTGCGAGAATGGCAACTCGTGCCCGGGTTGC
GGCGTGGAGTTCAAGACCTGCAACCCCGAGGCTTGCCCGGAAGTGCGACGCAACACACCCTGGACGCCCT
GGCTGCCCGTGAACGTGACCCAGGGTGGAGCGCGCCAGGAGCAGCGATTCCGCTTCACCTGCCGCGCGCC
GCTGCCGGACCCGCACGGTCTGCAGTTCGGCAAGAGGAGGACAGAGACCAGGACTTGCCCGGCAGACGGC
ACCGGAGCCTGCGACACCGACGCCCTGGTGGAGGATCTCCTGCGCAGCGGGAGCACGTCACCACACACTC
TGAACGGAGGCTGGGCCACCTGGGGCCCGTGGTCATCCTGCTCCCGGGACTGCGAGCTGGGCTTCCGCGT
CCGCAAGAGAACTTGTACCAACCCGGAGCCTCGCAACGGGGGCTTGCCCTGCGTGGGAGACGCTGCGGAG
TACCAAGACTGCAACCCACAGGCTTGCCCAGTGCGGGGTGCTTGGTCCTGCTGGACCGCATGGTCCCAGT
GCTCAGCATCCTGCGGTGGTGGCCACTATCAACGCACCCGTTCCTGCACCAGCCCCGCCCCATCCCCAGG
TGAGGACATCTGCCTCGGCCTGCACACGGAGGAGGCCCTATGTTCAACACAGGCCTGCCCAGAAGGCTGG
TCACTGTGGTCTGAGTGGGGTGTCTGCACTGAGGATGGGGCCCAGAGCCGGAGCCGGAGCTGTGAGGAGC
TTCTCCCAGGACCAGGTGCCTGTGTTGGCAACAGCAGCCAGAGCCGGCCCTGCCCCTACAGTGAGATTCC
TGTCATCCTACCTGCTTCCAGTGTGGAGGAGACCACCAGCTGTGGAGGGTTCAATCTCATCCACCTGATA
GTCACTGGTGTGTCCTGCTTCCTGGTTTCTGGGCTCTTGACCTTGGCAGTGTACCTGTCTTGCCAGCACT
GCCAGCGCCAGTCTCAGGAGTCCACGCTTGTCCATCCTGCCACACCTAACCACTTGCACTACAAGGGTGG
GGGCACCCCCAAGAATGAGAAGTACACCCCTATGGAATTCAAGACACTGAACAAGAACAACTTAATCCCT
GATGACAGAGCCAACTTCTACCCACTGCAGCAGACCAATGTGTATACAACCACGTACTACCCCAGCCCAC
TGAACAAGCCCAGCTTCCGGCCTGAGGCCTCACCTGGACAGCGCTGTTTCCCCAACAGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216182 representing NM_013661
Red=Cloning site Green=Tags(s)

MVVPGPLALSLLLSSLTLLVSHLSSSQDIASESSSEQQMCTRREHPIVAFEDLKPWVFNFTYPGVRDFSQ
LALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVSGRKVFM
CGTNAFSPVCSSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGS
GPPLRTAQYNSKWLNEPNFVAAFDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTT
FMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISKAFNGPFRYQEN
PRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDL
VQAKDTLYHVLYIGTESGTILKALSTASRSLRGCYLEELHVLPPGRLEPLRSLRILHSARALFVGLSDRV
LRVPLERCSAYHSQGACLGARDPYCGWDGKRQLCSTLEDSSNMSLWIQNITTCPVRNVTRDGGFGPWSPW
KPCEHLDGDNSGSCLCRARSCDSPRPRCGGLECLGPSIHIANCSRNGAWTAWSSWAQCSTSCGIGFQVRQ
RSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSNNCGGGVQSRRRSCENGNSCPGC
GVEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLPDPHGLQFGKRRTETRTCPADG
TGACDTDALVEDLLRSGSTSPHTLNGGWATWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAE
YQDCNPQACPVRGAWSCWTAWSQCSASCGGGHYQRTRSCTSPAPSPGEDICLGLHTEEALCSTQACPEGW
SLWSEWGVCTEDGAQSRSRSCEELLPGPGACVGNSSQSRPCPYSEIPVILPASSVEETTSCGGFNLIHLI
VTGVSCFLVSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIP
DDRANFYPLQQTNVYTTTYYPSPLNKPSFRPEASPGQRCFPNS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013661
ORF Size 3279 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013661.2, NP_038689.2
RefSeq Size 4556 bp
RefSeq ORF 3282 bp
Locus ID 20357
UniProt ID Q60519
Cytogenetics 16 B3
Summary May act as positive axonal guidance cues.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013661" in other vectors (4)
SKU Description Size Price
MC223507 Sema5b (untagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b), (10ug) 10 ug
$1,047.00
MR216182 Sema5b (Myc-DDK-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b) 10 ug
$589.00 $951.00
MR216182L3 Lenti ORF clone of Sema5b (Myc-DDK-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b) 10 ug
$1,251.00
MR216182L4 Lenti ORF clone of Sema5b (mGFP-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (Sema5b) 10 ug
$1,251.00

file-alt Citations

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