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Krt82 (NM_053249) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216167
Krt82 (tGFP-tagged) - Mouse keratin 82 (Krt82), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $681.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Krt82
Synonyms Krt2-20
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216167 representing NM_053249
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCATGCCGAAATTTCCAGTTGAGCCCCAGATGTGGCAACCGGAGTTTCAGCTCGTGCTCAGCCATCA
TGCCCCGGATGGTCACCCACTATGAAGTGAGCAAGGCGCCCTGTCGGTCCGGGGGTGGCGGGGGCCTCCG
AGCCCTGGGTTGCCTTGGCTCTCGGAGCCTGTGCAACGTGGGTTTCGGGAGGCCCCGTGTGGCCTCCAGA
TGCGGCATGCCAGGCTTCGGGTACCGAGCGGGAGCCACCTGTGGATCTTCTGCCTGCATCACCCCTGTCA
CCATCAATGAGAGTCTGCTGGTCCCCCTGGAGCTGGAGATTGACCCGACCGTGCAAAGGGTGAAGAGAGA
TGAAAAGGAGCAGATTAAGTGCCTGAATAACCGATTCGCATCCTTCATCAACAAGGTGCGGTTCCTGGAG
CAGAAAAACAAGCTTCTGGAGACCAAGTGGAACTTCATGCAGCAGCAGAGAAGTTGCCAGAGCAACATGG
AGCCGCTCTTCGAGGGCTACATCTGTGCCCTGCGGCGACAGCTGGACTGTGTGTCCGGGGACCACGGCAG
ACTAGAGGCCGAGCTCTGCAGTCTCCAGGAGGCGCTGGAGGGTTATAAGAAAAAGTACGAAGAGGAGCTG
TCTCTGCGTCCCTGTGCTGAGAATGAGTTTGTCACCCTGAAGAAGGACGTGGACACAGCCTTCCTGGTGA
AGGCTGACCTGGAAACCAACTTGGAGGCTCTAGAACATGAGATTGAGTTCCTGAAAGCCCTGTTTGAGGA
GGAGATCGGCCTGCTGCAGTCTCAGATCTCAGAGACTTCAGTCATCGTGAAGATGGACAACAGCCGGGAA
CTGGATGTGGACGGCATCGTAGCCGAGATCAAGGCCCAGTATGACGACATCGCCAGCCGCAGCAAAGCAG
AAGCAGAGGCCTGGTACCAGTGCCGGTATGAGGAGCTGAGGTTAACGGCTGGGAACCACTGTGACAACCT
ACGCAACCGCAAAAATGAGATCCTGGAAATGAACAAGCTAATCCAGCGGCTGCAGCAGGACATTGAGGCA
GTCAAAGGCCAGCGATGCAAGCTCGAGGGGGCCATTGCCCAGGCAGAGCAGCAGGGAGAGGCCGCCCTCA
CTGATGCCAAGTGCAAGCTGGCAGGGCTGGAGGAGGCCTTGCAGAAGGCCAAGCAGGACATGGCCTGCCT
GCTCAAGCAGTACCAAGAGGTGATGAACTGCAAGCTGGGGCTGGACATCGAGATCGCCACCTACAGGCGC
CTGCTGGAGGGCGAGGAGCACAGGTTGTGTGAAGGCATCGGGCCTGTGAATATCTCTGTCAGCAGCTCCA
AAGGTGCCGTACTCTACGAGCCCTGTGTGATGGGCACGCCCATGCTGAGGACCGAATACTGCACCGGGAC
CACGGGTGTCCTGAGGAACAGTGGGGGCTGCAACGTGGTGGGCACCGGTGAACTCTACATCCCCTGCGAG
CCCCAAGGGCTCCTGGGCTGTGGAAATGGGCGGAGCTCCAGCATGAAGGTGGGGGTGGGTAGCAATTCGT
GCAGCCGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216167 representing NM_053249
Red=Cloning site Green=Tags(s)

MSCRNFQLSPRCGNRSFSSCSAIMPRMVTHYEVSKAPCRSGGGGGLRALGCLGSRSLCNVGFGRPRVASR
CGMPGFGYRAGATCGSSACITPVTINESLLVPLELEIDPTVQRVKRDEKEQIKCLNNRFASFINKVRFLE
QKNKLLETKWNFMQQQRSCQSNMEPLFEGYICALRRQLDCVSGDHGRLEAELCSLQEALEGYKKKYEEEL
SLRPCAENEFVTLKKDVDTAFLVKADLETNLEALEHEIEFLKALFEEEIGLLQSQISETSVIVKMDNSRE
LDVDGIVAEIKAQYDDIASRSKAEAEAWYQCRYEELRLTAGNHCDNLRNRKNEILEMNKLIQRLQQDIEA
VKGQRCKLEGAIAQAEQQGEAALTDAKCKLAGLEEALQKAKQDMACLLKQYQEVMNCKLGLDIEIATYRR
LLEGEEHRLCEGIGPVNISVSSSKGAVLYEPCVMGTPMLRTEYCTGTTGVLRNSGGCNVVGTGELYIPCE
PQGLLGCGNGRSSSMKVGVGSNSCSR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053249
ORF Size 1548 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053249.3, NP_444479.2
RefSeq Size 2100 bp
RefSeq ORF 1551 bp
Locus ID 114566
UniProt ID Q99M74
Cytogenetics 15 56.9 cM
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Documents
Resources
"NM_053249" in other vectors (4)
SKU Description Size Price
MC217345 Krt82 (untagged) - Mouse keratin 82 (Krt82), (10ug) 10 ug
$529.00
MR216167 Krt82 (Myc-DDK-tagged) - Mouse keratin 82 (Krt82) 10 ug
$289.00 $481.00
MR216167L3 Lenti ORF clone of Krt82 (Myc-DDK-tagged) - Mouse keratin 82 (Krt82) 10 ug
$781.00
MR216167L4 Lenti ORF clone of Krt82 (mGFP-tagged) - Mouse keratin 82 (Krt82) 10 ug
$781.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.