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Pde1a (NM_001159582) Mouse Tagged ORF Clone

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SKU
MG216159
Pde1a (tGFP-tagged) - Mouse phosphodiesterase 1A calmodulin-dependent (Pde1a) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $707.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pde1a
Synonyms AI987702; AW125737
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216159 representing NM_001159582
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGTCTACTGATACGGACATCGAAGAATTGGAAAACGCCACTTACAAGTATCTCATTGGAGAACAGA
CCGAGAAGATGTGGCAGCGCCTGAAGGGAATACTAAGATGCTTAGTGAAGCAGCTGGAGAAAGGTGATGT
CAACGTTGTTGACCTAAAGAAGAATATCGAATATGCAGCATCAGTACTGGAAGCTGTTTACATCGATGAG
ACAAGAAGACTGCTGGACACAGAGGATGAACTCAGCGACATCCAGACTGACTCCGTCCCATCGGAAGTCC
GGGATTGGTTGGCTTCAACTTTCACACGCAAAATGGGGATGATGAAAAAGAAGCCTGAAGAAAAACCAAA
GTTTCGCAGCATTGTCCATGCTGTCCAGGCTGGGATTTTTGTGGAAAGGATGTACAGGAAGAACTATCAC
ATGGTTGGTTTGACATATCCTGCAGCTGTCATAGTGACACTCAAGGAAGTTGATAAATGGTCTTTTGATG
TATTTGCCTTAAATGAAGCAAGCGGGGAGCATAGCCTGAAGTTTATGATTTATGAACTCTTTACCAGATA
TGATCTTATCAACCGCTTCAAGATTCCTGTTTCTTGCCTAATTGCCTTTGCAGAAGCCTTAGAAGTTGGT
TACAGCAAGCACAAAAATCCATATCATAATTTGGTCCATGCAGCTGACGTCACTCAAACTGTGCATTATA
TAATGCTTCATACAGGTATCATGCACTGGCTCACTGAACTGGAAATTTTAGCCATGGTCTTTGCAGCTGC
CATTCATGATTATGAGCACACAGGAACAACAAACAACTTCCATATTCAGACGAGGTCGGACGTTGCTATT
CTGTACAACGACCGCTCAGTGCTTGAGAATCACCACGTCAGCGCAGCCTACAGACTTATGCAAGAGGAGG
AAATGAATATTTTGGTAAATTTGTCTAAAGATGACTGGAGGGATCTTCGGAACTTAGTGATTGAAATGGT
CTTAGCGACAGACATGTCAGGGCATTTTCAGCAAATTAAAAACATAAGAAACAGCTTGCAGCAGCCGGAA
GGGATTGACAGAGCCAAAACTATGTCCCTGATACTCCATGCAGCTGACATCAGCCACCCAGCCAAAACTT
GGAAGTTGCACTACAGATGGACCATGGCCCTAATGGAGGAGTTTTTCCTGCAGGGAGACAAAGAAGCTGA
ATTAGGGCTTCCATTTTCTCCACTTTGTGATCGGAAGTCAACAATGGTTGCCCAGTCGCAAATAGGTTTC
ATTGATTTCATAGTGGAGCCAACGTTTTCTCTCCTGACGGACTCAACAGAGAAAATTGTCATTCCTCTTA
TAGAGGAAGCTTCAAAGTCACAGTCTTCAAACTACGGGGCAAGCAGCTCGTCCACCATGATTGGGTTCCA
TGTTGCTGACTCTCTGAGGCGGTCAAATACAAAAGGCTCCGTGTGTGATGGGAGCTATGCCCCAGACTAC
TCGCTCTCAGCTGTGGATCTGAAGAGTTTCAAAAACAACCTGGTGGACATCATTCAGCAGAACAAAGAGA
GGTGGAAAGAGTTGGCTGCCCAAGGTGAACTTGATCTTCATAAGAACTCAGAAGAGTTAGGAAATACTGA
AGAAAAGCATGCTGACACACGTCCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216159 representing NM_001159582
Red=Cloning site Green=Tags(s)

MGSTDTDIEELENATYKYLIGEQTEKMWQRLKGILRCLVKQLEKGDVNVVDLKKNIEYAASVLEAVYIDE
TRRLLDTEDELSDIQTDSVPSEVRDWLASTFTRKMGMMKKKPEEKPKFRSIVHAVQAGIFVERMYRKNYH
MVGLTYPAAVIVTLKEVDKWSFDVFALNEASGEHSLKFMIYELFTRYDLINRFKIPVSCLIAFAEALEVG
YSKHKNPYHNLVHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAI
LYNDRSVLENHHVSAAYRLMQEEEMNILVNLSKDDWRDLRNLVIEMVLATDMSGHFQQIKNIRNSLQQPE
GIDRAKTMSLILHAADISHPAKTWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGF
IDFIVEPTFSLLTDSTEKIVIPLIEEASKSQSSNYGASSSSTMIGFHVADSLRRSNTKGSVCDGSYAPDY
SLSAVDLKSFKNNLVDIIQQNKERWKELAAQGELDLHKNSEELGNTEEKHADTRP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001159582
ORF Size 1635 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001159582.1, NP_001153054.1
RefSeq Size 4514 bp
RefSeq ORF 1638 bp
Locus ID 18573
UniProt ID Q61481
Cytogenetics 2 C3
Summary Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001159582" in other vectors (4)
SKU Description Size Price
MC218815 Pde1a (untagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 1, (10ug) 10 ug
$559.00
MR216159 Pde1a (Myc-DDK-tagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 1 10 ug
$289.00 $507.00
MR216159L3 Lenti ORF clone of Pde1a (Myc-DDK-tagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 1 10 ug
$807.00
MR216159L4 Lenti ORF clone of Pde1a (mGFP-tagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 1 10 ug
$807.00

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