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March9 (NM_001033262) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216142
41342 (tGFP-tagged) - Mouse membrane-associated ring finger (C3HC4) 9 (March9), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
  $886.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol March9
Synonyms AI608492; BC019560
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216142 representing NM_001033262
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCAAGTCTCGGCTCCGCATGTTCCTGAACGAGCTGAAGCTGCTGGTGCTGACGGGCGGGGGGCGGC
CCCGGGCCGAGCCGCAGCCCCGGGGGGGCGGGGGAGGCGGCTGCGGCTGGGCGCCCTTCGCCGGCTGCTC
CGCCCGGGACGGGGACGGCGACGAGGAGGAGTACTACGGGTCGGAGCCGCGGGCCCGGGGCCTGGCCGGC
GACAAGGAACCGCGGGCCGGACCCCCGCCGCCGCCCGCGCCGCCGCCGCCGCCCCCAGGCGCGCTGGACG
CTCTGTCGCTCAGCAGCAGCCTGGACAGTGGGCTCCGAACCCCCCAGTGCAGGATCTGCTTCCAAGGCCC
GGAGCAGGGGGAGCTCCTGAGCCCTTGCCGCTGTGACGGCTCAGTGCGTTGCACACACCAACCCTGCCTA
ATCCGCTGGATCAGTGAGAGGGGCTCATGGAGCTGTGAGCTTTGCTACTTCAAGTACCAGGTCCTGGCGA
TCAGCACCAAGAATCCACTGCAGTGGCAGGCCATCTCCCTGACCGTCATCGAGAAGGTCCAGATTGCTGC
TATCGTCCTGGGATCGCTGTTCCTCGTCGCCAGCATCTCCTGGCTCATCTGGTCCTCGCTCAGCCCTTCA
GCCAAGTGGCAGCGCCAGGACCTCCTCTTCCAGATTTGCTATGGCATGTACGGCTTCATGGATGTCGTCT
GCATAGGCCTCATCGTCCACGAAGGGTCTTCTGTTTACCGCATCTTCAAGCGCTGGCAGGCCGTGAACCA
GCAGTGGAAAGTCCTGAACTATGACAAGACCAAGGACGTGGGAGGAGACACAGGGGGAGGGGCGGCAGGG
AAGCCAGGCCCCAGGACCTCACGGACCAGCCCCCCTGCAGGGGCCCCCACCCGGCCCCCAGCAGCCCAGC
GCATGCGCATGCGGACGCTCTTGCCTCAGCGCTGTGGTTATACGATCCTGCATCTCCTCGGACAGCTGCG
ACCACCAGATGCCCGTTCCAGCTCTCATTCTGGTCGAGAGGTTGTCATGAGGGTCACCACAGTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216142 representing NM_001033262
Red=Cloning site Green=Tags(s)

MLKSRLRMFLNELKLLVLTGGGRPRAEPQPRGGGGGGCGWAPFAGCSARDGDGDEEEYYGSEPRARGLAG
DKEPRAGPPPPPAPPPPPPGALDALSLSSSLDSGLRTPQCRICFQGPEQGELLSPCRCDGSVRCTHQPCL
IRWISERGSWSCELCYFKYQVLAISTKNPLQWQAISLTVIEKVQIAAIVLGSLFLVASISWLIWSSLSPS
AKWQRQDLLFQICYGMYGFMDVVCIGLIVHEGSSVYRIFKRWQAVNQQWKVLNYDKTKDVGGDTGGGAAG
KPGPRTSRTSPPAGAPTRPPAAQRMRMRTLLPQRCGYTILHLLGQLRPPDARSSSHSGREVVMRVTTV

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033262
ORF Size 1044 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033262.2, NP_001028434.1
RefSeq Size 1998 bp
RefSeq ORF 1047 bp
Locus ID 216438
UniProt ID Q3TZ87
Cytogenetics 10 D3
Summary E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I, CD4 and ICAM1, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033262" in other vectors (4)
SKU Description Size Price
MC212640 41342 (untagged) - Mouse membrane-associated ring finger (C3HC4) 9 (March9), (10ug) 10 ug
$686.00
MR216142 41342 (Myc-DDK-tagged) - Mouse membrane-associated ring finger (C3HC4) 9 (March9) 10 ug
$686.00
MR216142L3 Lenti ORF clone of 41342 (Myc-DDK-tagged) - Mouse membrane-associated ring finger (C3HC4) 9 (March9) 10 ug
$986.00
MR216142L4 Lenti ORF clone of 41342 (mGFP-tagged) - Mouse membrane-associated ring finger (C3HC4) 9 (March9) 10 ug
$986.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.