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Ganc (NM_172672) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216095
Ganc (tGFP-tagged) - Mouse glucosidase alpha; neutral C (Ganc), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,036.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ganc
Synonyms 5830445O15Rik; 9330160A12; mFLJ00088
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216095 representing NM_172672
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGCAGCTGAGAAAGAGGAAATCAGTGTTGAAGATGAAGCAGTGGATAAAACTATTTTCAAAGACT
GTGGCAAGATTGCATTCTACAGGCGGCAGAAACAGCAGCTCACCAAGACTACCACCTACCAGGCTTTGCT
AGGCTCAGTCGACACAGAACAGGACAGCACCAGGTTCCAAATCATCAGTGAAGCAACTAAGATTCCTCTC
GTGGCTGAAGTTTATGGTATAGAGAAAGACATTTTCAGACTTAAGATCAATGAAGAGACTCCACTGAAAC
CAAGGTATGAAGTTCCTGACGTCATCAACAGCAAGCTAGGAACTGTCAGGCTGGTCTGCTCAGGGGACAC
AGGCAGCCTAATACTGACAAATAGAAAAGGAGATCTGAAATGTCACGTCTCAGCAAACCCATTCAAGATC
GATTTGCTGTCGAAGAACGAGGCTGTGATCAGCATCAACTCCCTGGGCCAGTTATACTTTGAGCACCTAC
AGGTTCCTCACAAGCAGAGAGCTACCAAAGGAAATGGGCAAAATACACCAGCTGCCACCTCTCAGGAAAA
TCAAGAGGACCTGGGCCTGTGGGAAGAAAAATTTGGAAAATTTGTGGATGTCAAAGCTAATGGCCCTTCC
TCTGTTGGTTTGGATTTCTCTCTTCATGGATTTGAACATCTCTATGGTATTCCTCAACATGCAGAGTCAC
ATCAACTTAAAAATACCAGAGATGGAGATGCATACCGTCTTTATAATCTGGACGTCTATGGATACCAAGT
GCATGACAAAATGGGCATATATGGTTCAGTGCCTTATCTCCTGGCCCACAAACAGGGCAGGACTGTAGGC
ATTTTCTGGCTGAATGCTTCAGAAACACTCGTGGAGATCAACACAGAGCCAGCAGTTGAGTACACACTGA
CCCAGATGGGCCCAGCAGCTGCCAAACAAAAGGTCAGGTGCCGAACTGATGTGCACTGGATGTCAGAGAG
TGGGATCATTGACGTTTTTCTGCTGACAGGACCTACACCTGCTGACGTCTTCAAACAGTACTCATATATC
ACAGGCACACAAGCCATGCCCCCTCTTTTTTCCTTGGGGTACCACCAGTGTCGCTGGAACTATGAAGATG
AACAGGATGTGAAGGCAGTAGATGCAGGGTTTGATGAGCATGACATTCCATATGATGTCATGTGGCTGGA
CATAGAGCACACGGAGGACAAGAAGTACTTCACCTGGGACAAAAAGAGATTTGCAAACCCCAAAAGGATG
CAAGAGCTGCTCAGGAGCAAAAAACGCAAGCTTGTGGTTATCAGTGACCCCCACATCAAGGTGGATCCTG
ACTACACAGTCTATGCTCAAGCCAAAGAACAGGGCTTCTTTGTGAAGAATCCAGAGGGTGGCGACTTCGA
AGGAGTATGTTGGCCTGGTCTCTCGTCTTACCTGGATTTCACCAACCCCAAGGTCAGAGAGTGGTATTCC
AGCCTTTTTGCTTTTCCTGTTTATCAGGGATCTACGGACATCCTTTTCCTATGGAATGACATGAATGAGC
CCTCAGTCTTCAGAGGGCCTGAGCTAACCATGCACAAGAGTGCTGTTCATTATGGCGACTGGGAACACAG
AGAACTTCACAACATCTACGGATTTTATCAGCAAATGGCTACTGCAGAAGGATTGATACAACGATCTAAG
GGGAAGGAGAGGCCCTTTGTTCTTTCACGTTCCTTCTTTGCCGGATCACAAAAGTATGGTGCAGTGTGGA
CAGGAGACAACAAAGCGGAATGGAGCTATCTGAAAATCTCCATCCCGATGTTACTCACTCTCAGTGTCTC
GGGAATCTCCTTCTGTGGAGCTGATGTGGGTGGCTTCATTGGCAATCCTGAGGCAGAGCTGCTTGTGCGT
TGGTACCAGGCTGGAGCTTACCAGCCCTTCTTCCGAGGCCATGCCACCATGAACACCAAGCGACGAGAAC
CCTGGCTGTTTGGGGAAGAATACACTCAGCTGATCCGGGAAGCCATCAGGCAGCGCTACGCCCTCCTGCC
CTATCTGTATTCTCTGTTCTACCACACGCACGTGTCTTCTCAGCCCGTCATGAGGCCTCTGTGGGTCGAA
TATCCTGATGACTTAGAGACTTTTGCTGTGGAAGATGAATACATGCTGGGAAGCGCTTTATTGGTTCATC
CAGTTACAGACCCACAGACCGCCACGATTGACGTGTTCCTGCCAGGGTCAGATGAGGTCTGGTACGACTC
TAAGACATTTGCATATTGGAAAGGAGGGTGTACTGTGAAGATTCCAGTAACCTTGGATACTATCCCAGTG
TTTCAGCGAGGTGGAAGTGTGGTACCAGTAAAGACCACTGTGGGAACGTCCACTGGCTGGATGGCTGACT
CCCCTTATGAGCTCCGGGTTGCCCTAAGCACTCAGGGTTCTGCTGTGGGAGAGCTGTATCTTGATGACGG
CCATTCATTCCAGTACCTCCACCAGAACCAGTTCTTATACCGCAAGTTCTTATTCTGCTCCAGTGTTCTG
ACCAACAGGTGTGCCAATGAGAAGGGTCATTACCCCAGCAAGTGCATTGTGGAGCAGATCTTGGTCTTAG
GCCTTAAGAAGAAGCCATCTTCTGTGACTACCCACTTGTCTGATGGTAGAGCTCAGCCTGCAGCTTTTAC
ATATTGTGCCGAAACATCTGCCCTGCGTCTGGAGAAGCTGTCTCTGCGCATTGGTGAGGACTGGGAAGTC
CGTGTGGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216095 representing NM_172672
Red=Cloning site Green=Tags(s)

MEAAEKEEISVEDEAVDKTIFKDCGKIAFYRRQKQQLTKTTTYQALLGSVDTEQDSTRFQIISEATKIPL
VAEVYGIEKDIFRLKINEETPLKPRYEVPDVINSKLGTVRLVCSGDTGSLILTNRKGDLKCHVSANPFKI
DLLSKNEAVISINSLGQLYFEHLQVPHKQRATKGNGQNTPAATSQENQEDLGLWEEKFGKFVDVKANGPS
SVGLDFSLHGFEHLYGIPQHAESHQLKNTRDGDAYRLYNLDVYGYQVHDKMGIYGSVPYLLAHKQGRTVG
IFWLNASETLVEINTEPAVEYTLTQMGPAAAKQKVRCRTDVHWMSESGIIDVFLLTGPTPADVFKQYSYI
TGTQAMPPLFSLGYHQCRWNYEDEQDVKAVDAGFDEHDIPYDVMWLDIEHTEDKKYFTWDKKRFANPKRM
QELLRSKKRKLVVISDPHIKVDPDYTVYAQAKEQGFFVKNPEGGDFEGVCWPGLSSYLDFTNPKVREWYS
SLFAFPVYQGSTDILFLWNDMNEPSVFRGPELTMHKSAVHYGDWEHRELHNIYGFYQQMATAEGLIQRSK
GKERPFVLSRSFFAGSQKYGAVWTGDNKAEWSYLKISIPMLLTLSVSGISFCGADVGGFIGNPEAELLVR
WYQAGAYQPFFRGHATMNTKRREPWLFGEEYTQLIREAIRQRYALLPYLYSLFYHTHVSSQPVMRPLWVE
YPDDLETFAVEDEYMLGSALLVHPVTDPQTATIDVFLPGSDEVWYDSKTFAYWKGGCTVKIPVTLDTIPV
FQRGGSVVPVKTTVGTSTGWMADSPYELRVALSTQGSAVGELYLDDGHSFQYLHQNQFLYRKFLFCSSVL
TNRCANEKGHYPSKCIVEQILVLGLKKKPSSVTTHLSDGRAQPAAFTYCAETSALRLEKLSLRIGEDWEV
RVG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172672
ORF Size 2739 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172672.2, NP_766260.2
RefSeq Size 4818 bp
RefSeq ORF 2742 bp
Locus ID 76051
UniProt ID Q8BVW0
Cytogenetics 2 E5
Summary Has alpha-glucosidase activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172672" in other vectors (4)
SKU Description Size Price
MC222596 Ganc (untagged) - Mouse glucosidase, alpha, neutral C (Ganc), (10ug) 10 ug
$920.00
MR216095 Ganc (Myc-DDK-tagged) - Mouse glucosidase, alpha, neutral C (Ganc) 10 ug
$589.00 $836.00
MR216095L3 Lenti ORF clone of Ganc (Myc-DDK-tagged) - Mouse glucosidase, alpha; neutral C (Ganc) 10 ug
$1,136.00
MR216095L4 Lenti ORF clone of Ganc (mGFP-tagged) - Mouse glucosidase, alpha; neutral C (Ganc) 10 ug
$1,136.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.