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Prpf4b (NM_013830) Mouse Tagged ORF Clone

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SKU
MG216087
Prpf4b (tGFP-tagged) - Mouse PRP4 pre-mRNA processing factor 4 homolog B (yeast) (Prpf4b), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,077.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Prpf4b
Synonyms 2610037H07; cbp143; Prp4; Prp4k; Prpk
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216087 representing NM_013830
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCCACGGAGCCCCCGTCCCTGCGGGAGCAAGCTGAGATGGATGATGCTGATAATTCTGAAAAGA
GTGTAAATGAAGAAAATGGAGAGGTATCAGAAGACCAATCTCAAAATAAACACAGTCGTCACAAAAAAAA
GAAGCATAAACACAGAAGTAAACATAAGAAACATAAACATTCCTCAGAAGAAGATAGGGATAAAAAACAC
AAACATAAGCATAAGCATAAGAAGCACAAACGGAAAGAAGTTATCGAGGCTTCCGACAAAGAAGGCCTGT
CCCCTGCAAAAAGAACTAAGCTTGATGATTTAGCTTTGCTGGAAGACTTGGAAAAGCAGAGAGCCTTGAT
TAAAGCTGAGCTTGATAATGAGTTAATGGAGGGGAAGGTCCAGTCTGGAATGGGGCTCATTTTGCAAGGC
TATGAGTCTGGCTCCGAAGAAGAAGGAGAGATTCACGAAAAGGCAAGAAATGGGAACAGGTCCAGTACCA
GGTCTTCCAGTACTCGGGGAAAACTTGAAATTACGGACAACAAAAATAGTGCAAAGAAACGAAGTAAGAG
CAGATCCAAAGAACGGACCAGGCACAGATCGGACAAAAGGAAAAGCAAGGGGGCTGGGGAAATGCTGAGA
GAGAAAGCAAACAGGAGCAAGTCCAAGGAGAGGAGAAAATCCAAAAGCCCATCCAAAAGAAGCAAGTCTC
AAGACCAAGCTAGAAAGTCAAAGTCCCCTCCACTCAGACGGCGGTCCCAAGAGAAAGTGGGGAAGGCCAG
ATCTCCTGCTGAGGAGAAGATGAAGAGTGAAGAGAAAGGCAAAATAAAGGATAGGAAAAAATCACCAATT
GTAAATGAAAGGAGTCGAGATAGATCTAAAAAATCCAAGTCCCCAGTTGACTTAAGAGATAAATCCAAAG
ACAGAAGGTCAAGGTCCAAAGAGCGGAAGTCAAAACGATCTGAAATTGACAAAGAAAAGAAGCCAATTAA
ATCACCTTCAAAGGATGCTTCTTCTGGGAAGGAAAATAGGTCACCCAGTAGAAGACCTGGCCGTAGTCCT
AAAAGAAGAAGTTTGTCTCCAAAACTGCGAGACAAGTCAAGAAGAAGTAGGTCTCCGCTTCTCAATGACA
GAAGATCTAAGCAGAGCAAATCGCCTTCTCGAACTCTGTCTCCAGGCAGAAGGGCCAAGAGCCGATCCTT
AGAAAGAAAACGGAGAGAACCAGAGAGGAGACGACTTTCTTCTCCGAGAACAAGACCTCGAGATGATATC
CTTGGTAGATGTGAACGGTCAAAAGATGCCAGCCCCATCAATAGGTGGTCTCCAACCCGAAGAAGAAGTA
GGTCTCCAATTAGAAGGAGGTCCCGTTCCCCATTGAGACGCAGCAGGTCTCCTAGAAGAAGAAGCAGGTC
TCCTCGGAGAAGAGATAGGAGCCGAAGGAGCAGGTCACGCTTGAGGAGAAGGTCTCGATCACGGGGTGGT
CATAGGAGAAGGAGCAGAAGCAAAGTAAAGGAAGATAAATTTAAAGGAAGTCTCTCAGAAGGAATGAAAG
TTGAGCAAGAGTCTTCATCTGATGATAACCTTGAAGATTTTGATGTGGAGGAGGAAGATGAAGAGGCTCT
AATAGAACAGAGAAGAATACAGAGGCAAGCAATTGTGCAGAAATATAAATATCTTGCTGAAGATAGCAAT
ATATCTGTGCCATCTGAGCCAAGCAGCCCCCAGAGCAGCACAAGATCACGATCTCCTTCGCCAGACGACA
TCTTGGAGCGAGTTGCTGCTGATGTAAAAGAATATGAGCGGGAGAATGTTGACACCTTTGAGGCCTCAGT
AAAAGCCAAGCATAATCTAATGACAGTTGAACAGAATAATGGCTCATCTCAGAAGAAGATTTTGGCACCT
GACATGTTTACAGAATCTGATGACATGTTTGCTGCTTATTTTGATAGTGCTCGTCTCCGAGCTGCTGGCA
TTGGGAAAGATTTCAAAGAAAATCCCAACCTCAGAGATAATTGGACAGATGCAGAAGGCTATTACCGTGT
GAACATCGGGGAAGTCCTAGATAAACGTTATAATGTGTATGGCTACACTGGGCAGGGTGTGTTCAGCAAT
GTTGTGCGTGCCAGGGATAATGCGAGAGCCAACCAGGAAGTGGCTGTAAAGATCATCAGGAACAATGAAC
TCATGCAAAAGACTGGTTTAAAAGAGCTAGAATTCTTGAAAAAACTTAATGATGCTGATCCTGATGACAA
GTTCCATTGCCTGCGCCTCTTCAGACACTTTTACCACAAACAGCACCTCTGTCTCGTCTTTGAACCTCTC
AGTATGAATTTGCGAGAGGTATTAAAAAAATATGGTAAGGATGTCGGTCTCCATATTAAGGCTGTACGAT
CGTACAGTCAGCAGTTGTTCCTGGCATTGAAACTCCTTAAAAGATGTAATATCTTACATGCAGATATCAA
ACCGGATAATATTCTGGTTAATGAATCCAAAACAATTTTAAAACTTTGTGATTTTGGATCGGCCTCACAT
GTTGCAGATAATGACATTACGCCTTATCTTGTCAGTAGATTTTATCGTGCTCCGGAGATCATTATAGGTA
AAAGCTATGACTACGGTATCGATATGTGGTCTGTAGGTTGTACCTTATATGAACTCTACACTGGAAAAAT
CTTATTCCCTGGCAAAACTAATAACCATATGTTGAAGCTCGCCATGGACCTTAAAGGAAAAATGCCAAAT
AAGATGATCCGGAAAGGTGTATTCAAAGATCAACATTTTGACCAAAATCTGAACTTCATGTACATAGAGG
TTGATAAAGTGACAGAGAGGGAGAAAGTTACTGTCATGAGCACCATTAATCCAACTAAGGACCTGTTGGC
TGACTTGATTGGGTGCCAGCGACTTCCTGAAGATCAACGTAAAAAAGTACACCAGCTAAAGGACTTGTTG
GACCAGATCCTAATGTTGGACCCAGCTAAGCGAATTAGCATCAACCAGGCCCTGCAGCATGCATTCATCC
AGGAAAAAATT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216087 representing NM_013830
Red=Cloning site Green=Tags(s)

MAATEPPSLREQAEMDDADNSEKSVNEENGEVSEDQSQNKHSRHKKKKHKHRSKHKKHKHSSEEDRDKKH
KHKHKHKKHKRKEVIEASDKEGLSPAKRTKLDDLALLEDLEKQRALIKAELDNELMEGKVQSGMGLILQG
YESGSEEEGEIHEKARNGNRSSTRSSSTRGKLEITDNKNSAKKRSKSRSKERTRHRSDKRKSKGAGEMLR
EKANRSKSKERRKSKSPSKRSKSQDQARKSKSPPLRRRSQEKVGKARSPAEEKMKSEEKGKIKDRKKSPI
VNERSRDRSKKSKSPVDLRDKSKDRRSRSKERKSKRSEIDKEKKPIKSPSKDASSGKENRSPSRRPGRSP
KRRSLSPKLRDKSRRSRSPLLNDRRSKQSKSPSRTLSPGRRAKSRSLERKRREPERRRLSSPRTRPRDDI
LGRCERSKDASPINRWSPTRRRSRSPIRRRSRSPLRRSRSPRRRSRSPRRRDRSRRSRSRLRRRSRSRGG
HRRRSRSKVKEDKFKGSLSEGMKVEQESSSDDNLEDFDVEEEDEEALIEQRRIQRQAIVQKYKYLAEDSN
ISVPSEPSSPQSSTRSRSPSPDDILERVAADVKEYERENVDTFEASVKAKHNLMTVEQNNGSSQKKILAP
DMFTESDDMFAAYFDSARLRAAGIGKDFKENPNLRDNWTDAEGYYRVNIGEVLDKRYNVYGYTGQGVFSN
VVRARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDKFHCLRLFRHFYHKQHLCLVFEPL
SMNLREVLKKYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASH
VADNDITPYLVSRFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLKLAMDLKGKMPN
KMIRKGVFKDQHFDQNLNFMYIEVDKVTEREKVTVMSTINPTKDLLADLIGCQRLPEDQRKKVHQLKDLL
DQILMLDPAKRISINQALQHAFIQEKI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013830
ORF Size 3021 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013830.3
RefSeq Size 4482 bp
RefSeq ORF 3024 bp
Locus ID 19134
UniProt ID Q61136
Cytogenetics 13 A3.3
Summary Has a role in pre-mRNA splicing. Phosphorylates SF2/ASF.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013830" in other vectors (4)
SKU Description Size Price
MC223174 Prpf4b (untagged) - Mouse PRP4 pre-mRNA processing factor 4 homolog B (yeast) (Prpf4b), (10ug) 10 ug
$965.00
MR216087 Prpf4b (Myc-DDK-tagged) - Mouse PRP4 pre-mRNA processing factor 4 homolog B (yeast) (Prpf4b) 10 ug
$589.00 $877.00
MR216087L3 Lenti ORF clone of Prpf4b (Myc-DDK-tagged) - Mouse PRP4 pre-mRNA processing factor 4 homolog B (yeast) (Prpf4b) 10 ug
$1,177.00
MR216087L4 Lenti ORF clone of Prpf4b (mGFP-tagged) - Mouse PRP4 pre-mRNA processing factor 4 homolog B (yeast) (Prpf4b) 10 ug
$1,177.00

file-alt Citations

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