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Wdr89 (NM_028203) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216085
Wdr89 (tGFP-tagged) - Mouse WD repeat domain 89 (Wdr89), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wdr89
Synonyms 2600001A11Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216085 representing NM_028203
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGATTGAGGACCAGTTTGCTAGCCTGCATATTGTTAGGAGGTCCTCGGAACCAAAAGAACCCA
CTTACCTGCTTGGCATAGACACGTCAAAGACTGTACAAGCAGACAAAGGAGGCTTGGTGGCTGTTCTGTG
CTCAAATGGGTCGATAAGAATATATGATAAGGAGACACTACATCTGCTACGAGAATTTGGTGGCTCTCCC
GGGCTTCTTAGTGGAGTCAGCTTTGCAAATTCCTGTGACAGTGTCTACTCTGCAAGCACAGATGGCACCG
TGAAGTGTTGGGACGCTCGGGGAGCTAGCGAAAAGCCTGTGCAGTTGTTCAAGGGTTACCCTTCCTGTAG
TTTCATCAGCTTTGATGTCAACTGCAAAGATCATGTCATTTGTGCTGGTGCAGAGAAAGTTGACGAGGAT
GCATTGTTGGTATTTTGGGATGCAAGGTTCACATCTCAGGATCTGTCGACGAGAGACCCACTTGGTGCCT
ACTCAGAGACGCACAGTGATGATATCACTCAAGTGCGTTTCCATCCCAGCAATCCCAACTTGGTTGTCTC
AGGGTCCACTGATGGTCTGGTCAATGTGTTTGATCTCAGTGCTGATAAGGAAGAGGACGCACTGGTTGCC
ACCTGTAACTCCGTTTCATCAGTGAGCTGTATCGGTTGGTGTGGAAAAGATTATAAGCAAATCTACTGCA
TGACACACGATGAAGGGTTTTGTTGGTGGGATCTCAATCATCTGGATACAGATGAACCGATCACGTGTTT
GAACATCCAGGATGTAAGAGAAATAACCGATGTGAAAGATGGGCACTTGGACTATCTGATTGGTGGCCTG
TATCATGAGAAGATGGACAGACTGTTTGTTATTGGAGGAACCAACACCGGAAAGATTCACCTACTGAGCT
GCACCTCGGCAGGACTGACCCATGTGACCAGCCTTCATGGGGGCCATGCAGCCACCGTCCGTTCTTTCTG
TTGGAACGTGTCTGAGGATTCTCTGCTGACCGGAGGTGAAGATGCACAGCTATTGCTGTGGAAACCTGGA
GCTATGGAGAAGACGTTCACAAAGAAAGACAGCTTGAAAATTGCATCCTCCGTGCAGCAGCGAGTTCGAG
TCCACAGCAGCGATTCTTACAAGAAAAGGAAGCAGCAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216085 representing NM_028203
Red=Cloning site Green=Tags(s)

MEKIEDQFASLHIVRRSSEPKEPTYLLGIDTSKTVQADKGGLVAVLCSNGSIRIYDKETLHLLREFGGSP
GLLSGVSFANSCDSVYSASTDGTVKCWDARGASEKPVQLFKGYPSCSFISFDVNCKDHVICAGAEKVDED
ALLVFWDARFTSQDLSTRDPLGAYSETHSDDITQVRFHPSNPNLVVSGSTDGLVNVFDLSADKEEDALVA
TCNSVSSVSCIGWCGKDYKQIYCMTHDEGFCWWDLNHLDTDEPITCLNIQDVREITDVKDGHLDYLIGGL
YHEKMDRLFVIGGTNTGKIHLLSCTSAGLTHVTSLHGGHAATVRSFCWNVSEDSLLTGGEDAQLLLWKPG
AMEKTFTKKDSLKIASSVQQRVRVHSSDSYKKRKQQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028203
ORF Size 1158 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028203.1, NP_082479.1
RefSeq Size 2981 bp
RefSeq ORF 1161 bp
Locus ID 72338
UniProt ID Q9D0R9
Cytogenetics 12 C3
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Documents
Resources
"NM_028203" in other vectors (4)
SKU Description Size Price
MC211319 Wdr89 (untagged) - Mouse WD repeat domain 89 (Wdr89), (10ug) 10 ug
$503.00
MR216085 Wdr89 (Myc-DDK-tagged) - Mouse WD repeat domain 89 (Wdr89) 10 ug
$289.00 $457.00
MR216085L3 Lenti ORF clone of Wdr89 (Myc-DDK-tagged) - Mouse WD repeat domain 89 (Wdr89) 10 ug
$757.00
MR216085L4 Lenti ORF clone of Wdr89 (mGFP-tagged) - Mouse WD repeat domain 89 (Wdr89) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.