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Ckap2 (NM_001004140) Mouse Tagged ORF Clone

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SKU
MG216079
Ckap2 (tGFP-tagged) - Mouse cytoskeleton associated protein 2 (Ckap2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $819.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ckap2
Synonyms AI461656; AW228814; LB1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216079 representing NM_001004140
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGTCCAGGAAACGCTTCCTTGGCAGGGCTGCGAGGAATCCCTTGCCGGTGACCCGGGACCTGC
AGCTGCCCCCGACCAGGCGAGACCAGCCGGCCTTCCGAGAACAAAGAAAACAAAAACTCAAGGAATATCT
GTTAATACGAAAAACAGTGTTTCCATACAAACAAGAAAATCAGATATCCAGAGATCAGAAAATGATAACA
TCTGAGGATCGGGTCCAAGAAGGGAAAAAAGTTGTGAAACTCAAAACAGAAGTGGCTGATAAAGAAAATA
TTGAAAGTACAGTTGAGAAAAACTGCATTCCATTAAAAGCTGGAGAAGTAACCAGCTCAGAAATACATAA
TTCAAAGGATAATGTTCAAGCTGTGCAGTTATTATCTACAAGAGATGATTTGCCAGGTCAGACTGTGACA
TTAGACCCGGCATGTCACCATAAAGACAATAAAAAGATGCAAATGACTGCAGAGAAGCCCAAACAAGACA
GTAATGTGTCTAAAAAGCGAGTGCTTGGCTATTATCATGGCCAGATTGTTCAGTCTAAGATTAATTCATT
TAGAAAGCTTCCCAGTGTCAAAGGCGAGAGTCTTACAACAACCAAGAAACTTCCCACTACTGTGTCTAAA
GCCATGAAGGCTCAGAGTGAGCCTGCGAACACCGTCAGCGTAAAAGCCTCAACTACAGCTGCTGCCACCA
AGTTTGCAGACGCTAAACCTGTGAGCACTGCGTCGAAGGACACACTTGTGCGACCACCTATCCGAAGCCT
CCACAGTAGTTCCCACGGAGCTGCGAAACAGGGCCTCAGCAGGCCGCTTGCCAATGTTACAGTCAGAAAA
GGGATGCTTGACAAGGAGTCACATCGATCAGAACCAGTTGTGTCCGTTGTCAAAGCCGGTTCTTCTCAGG
CCCCATCAAGAAGCATAGCATCTAAAGATGCAGCCAGGACTGATTCGTCTAATACCAGACTGATGGTAAA
GCCAAAAGATACTGACCAGCGCAGATACACGATTGCAGGAGCAAGTGTTCATAGATCAGCTCAGCTCAAG
GACACCACCGCAGAGAGGAAAGCTCAGATGACGGAGTGGAGAACTGGCAAAGGAAAGGGGCTAAAACGAC
CTCCTCATTCAGTAGTCACCCAGGCTGAGCCAAAGGGACAAAGTGAAAACCCAGTTGGGTCCTTTTGGAC
TACCATGGCAGAAGAAGATGAGCAGAGATTATTTACTGAAAAAGTAAACAAAACTATTTCTGAATGCCTG
AACCTTATTAATGAGGGATGCCCAAAAGAAGAAATTTTGGCTACATTGAATGACCTGATTCATAATATTC
CAGATGCCAAAAAACTTGTTAAATATTGGATATGTCTTGTACGTATTGAACCAATCACAAGTCCTATTGA
AAATATTATCTCAATCTATGAGAAGGCCATCCTGGCAGGGGCTCAGCCTATTGAAGAGATGCGTCATATA
ATCATAGATATTCTAACAACGAAGAGTCAAGAAAAAGTCAATTTGGGAGAAAATATTGAGGAGGCTCATG
CAACCAAGGAACCTATCCAAGAAGTAAATGCTGATGCTAATGTAGGGTCAGGAAAACCAGGAGAGGAGAA
TGAACATCATGGCAAAGTTGAGGTGTATGAAGATGATCAAGACAACAAAATAAAAGATCCGGATTTAACA
ACTCCGGACTCAAAAACTGAAGCAGGTTGCATAATTCGATATAATGTATCTTCTACACCTCGGCTGCAAA
GTATGAAAAAGATGCAACATGATAAAAATTCCACACTTAAAGAGCTAAAGTTCCTGACACCAGTGAGACG
CTCACGGCGCATTCAAGACAAGACTTCACGACTGCCAGCCATGTTAAAAGACCACGACCCTTCTGTGTCT
TCCCTGGAGCAGTTGTCTGAGTTGGGAGGGGATGCCTTTGTCTGCCGCCCTAATGCAGCACTGTGCCCTC
TGTTCTTTGAGACCGACGTAGCGGAAGAGGAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216079 representing NM_001004140
Red=Cloning site Green=Tags(s)

MAESRKRFLGRAARNPLPVTRDLQLPPTRRDQPAFREQRKQKLKEYLLIRKTVFPYKQENQISRDQKMIT
SEDRVQEGKKVVKLKTEVADKENIESTVEKNCIPLKAGEVTSSEIHNSKDNVQAVQLLSTRDDLPGQTVT
LDPACHHKDNKKMQMTAEKPKQDSNVSKKRVLGYYHGQIVQSKINSFRKLPSVKGESLTTTKKLPTTVSK
AMKAQSEPANTVSVKASTTAAATKFADAKPVSTASKDTLVRPPIRSLHSSSHGAAKQGLSRPLANVTVRK
GMLDKESHRSEPVVSVVKAGSSQAPSRSIASKDAARTDSSNTRLMVKPKDTDQRRYTIAGASVHRSAQLK
DTTAERKAQMTEWRTGKGKGLKRPPHSVVTQAEPKGQSENPVGSFWTTMAEEDEQRLFTEKVNKTISECL
NLINEGCPKEEILATLNDLIHNIPDAKKLVKYWICLVRIEPITSPIENIISIYEKAILAGAQPIEEMRHI
IIDILTTKSQEKVNLGENIEEAHATKEPIQEVNADANVGSGKPGEENEHHGKVEVYEDDQDNKIKDPDLT
TPDSKTEAGCIIRYNVSSTPRLQSMKKMQHDKNSTLKELKFLTPVRRSRRIQDKTSRLPAMLKDHDPSVS
SLEQLSELGGDAFVCRPNAALCPLFFETDVAEEE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001004140
ORF Size 1992 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001004140.2, NP_001004140.2
RefSeq Size 2601 bp
RefSeq ORF 1995 bp
Locus ID 80986
UniProt ID Q3V1H1
Cytogenetics 8 A2
Summary Possesses microtubule stabilizing properties. Involved in regulating aneuploidy, cell cycling, and cell death in a p53-dependent manner.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001004140" in other vectors (4)
SKU Description Size Price
MC220276 Ckap2 (untagged) - Mouse cytoskeleton associated protein 2 (Ckap2), (10ug) 10 ug
$681.00
MR216079 Ckap2 (Myc-DDK-tagged) - Mouse cytoskeleton associated protein 2 (Ckap2) 10 ug
$289.00 $619.00
MR216079L3 Lenti ORF clone of Ckap2 (Myc-DDK-tagged) - Mouse cytoskeleton associated protein 2 (Ckap2) 10 ug
$919.00
MR216079L4 Lenti ORF clone of Ckap2 (mGFP-tagged) - Mouse cytoskeleton associated protein 2 (Ckap2) 10 ug
$919.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.