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Bpifa5 (NM_025990) Mouse Tagged ORF Clone

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SKU
MG216078
Bpifa5 (tGFP-tagged) - Mouse RIKEN cDNA 2310021H06 gene (2310021H06Rik), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Bpifa5 or NM_025990"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bpifa5
Synonyms 2310021H06Rik; 2310074B19Rik; Pluncl; Splunc5; Tpl
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216078 representing NM_025990
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTCTAGCTGGGAGCTTCATTGTCCTCTGTGGGCTGCTGGCCCAGAGCACAGCCCAGCTGGCAGGAC
TGCCCTATCCCCTGGGCCAAGATCTGCCCATGTCCATGGGTCACTGCCGGTCCTTGCACGTGGGCCAGAC
CCTGCCCTACTACGGAGTGACTCCAGTCGTGTCTACATATCCTTCAGACCATCTTGATAGAAACTTCAGA
GATGCTTTCAGACACGGCCTGCTTTCCGGAGGGATTCTGAGCTTTCTGGAACACATACCACTCTTAAACT
ATGTGAGACCTACAGGAAGCAATGCCGGTGGCCTGGTTGGTGTGCTTGGAAAAGTGATTTCATCAATCCC
TCTCTTGAACAACATCCTTGACATAAGAGTCACTAATCCCCAGCTACTGGAAATTGGTCTTGTGCAGAGC
TATGATTTCCACCGCCTCTATGTCACCATCCCACTGGGCTTTGATCTCAGAGTGAACACACTGGTGGTTG
GAAGTCTGTTGGAGCTATCTGTGAAGCTAGATGTCACTGCAGAAGTCTATGCTGTGAGAGATTCTTATGG
GAGGAGTCGCCTGGTCATTGGTGACTGTATATACCCTCCTGGCAGCTTGCGTATCTCCTTGCTTAATAGA
CTTGGTCCCCTGCAAAACCTTATAGACAGCCTCACAGACATCTTGACTAGAGTCATTCCTGGCCTGGTGC
AGGGTGTGGTGTGTCCTCTGGTCAATGGGGTTCTCAGCCTCTTGGACGTCACCCTAGCACATGATGTTGC
TGATGCGCTGCTGCGTGGAGTACAGTTTGTCATTAAGGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216078 representing NM_025990
Red=Cloning site Green=Tags(s)

MFLAGSFIVLCGLLAQSTAQLAGLPYPLGQDLPMSMGHCRSLHVGQTLPYYGVTPVVSTYPSDHLDRNFR
DAFRHGLLSGGILSFLEHIPLLNYVRPTGSNAGGLVGVLGKVISSIPLLNNILDIRVTNPQLLEIGLVQS
YDFHRLYVTIPLGFDLRVNTLVVGSLLELSVKLDVTAEVYAVRDSYGRSRLVIGDCIYPPGSLRISLLNR
LGPLQNLIDSLTDILTRVIPGLVQGVVCPLVNGVLSLLDVTLAHDVADALLRGVQFVIKA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025990
ORF Size 810 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025990.4, NP_080266.2
RefSeq Size 1085 bp
RefSeq ORF 813 bp
Locus ID 67135
UniProt ID Q9CQX3
Cytogenetics 2 H1
Summary May play a role in innate immunity in the oral cavity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025990" in other vectors (4)
SKU Description Size Price
MC210494 Bpifa5 (untagged) - Mouse RIKEN cDNA 2310021H06 gene (2310021H06Rik), (10ug) 10 ug
$330.00
MR216078 Bpifa5 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2310021H06 gene (2310021H06Rik) 10 ug
$289.00 $300.00
MR216078L3 Lenti ORF clone of Bpifa5 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2310021H06 gene (2310021H06Rik) 10 ug
$600.00
MR216078L4 Lenti ORF clone of Bpifa5 (mGFP-tagged) - Mouse RIKEN cDNA 2310021H06 gene (2310021H06Rik) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.