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Myrip (NM_144557) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216068
Myrip (tGFP-tagged) - Mouse myosin VIIA and Rab interacting protein (Myrip), (10ug)
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ORF Plasmid:
tGFP DDK
  $985.00
3 Weeks*
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Other products for "Myrip or NM_144557"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Myrip
Synonyms A230081N12Rik; Slac2-c; Slac2c
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216068 representing NM_144557
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAGGAAGCTGGACCTGTCGGGTCTGACCGACGATGAGACCGAGCATGTTCTCCAAGTGGTGCAGA
GAGACTTCAATCTTCGCAAAAAGGAGGAGGACCGGCTAAGTGAGATGAAGCAGAGGCTGGCCGAGGAGAA
CAGCAAGTGCAGCATCCTCTCGAAGCACCAGAAGTTCGTGGAGCGCTGCTGCATGCGGTGCTGCTCGCCC
TTCACCTTCCTGGTCAACGCCAGGCGCCGCTGCGGGGAATGCAAGTTCAGCGTCTGCAAGAGCTGCTGCT
CCTACCAGAAGCACGAGAAGCTATGGGTTTGCTGTGTCTGCCAGCAAGCCAGGCTTCTGAGGACACAGTC
TCTGGAGTGGTTCTACAACAATGTGAAAAGCCGCTTCAAGCGCTTTGGCAGTGCCAAGGTCCTGAAGAAC
CTGTACAGAAAACACCGTCTGGAGAGCGGTGCCTGCTTCGACATCCTAGGAGGAGGATTGTTTGAACCAA
ACCTGGAGAATGAAGGGAGCATTTCAGGAAGTGACTCCACGTTCTACAGGCAGTCAGAAGGGCACAGCAT
GATGGACACCTTGGCTGTGGCCCTACGAGTGGCAGAAGAGGCCATCGAGGAGGCCATTTCCAAAGCAGAA
TCCCATGGGGACAGCCTGGACAAACAGAACGAGGCCAGCTACCTGCGGGACCACAAGCAAGAGCTAACTG
AGGAGCTGGCCGGCACCATCCTGCAGAGGATTATAAGAAAGCAGAAGGATAAAGCTGAGCTGCGAGCTGA
GGAGGAGGAGCCAGAGTGGCCACGGTCCCAGAGTGGCAGTGTGAAGGCCAGGGGTGAGGGGACCACGGCC
CCTCCAGGACGACACAAAGCACGTGCCACCTTCAGGCGGTCCCAGTCTGCATTCTCCTTCACTATGGAAG
ATGCTCTGAAGTCTGGTTCAGCAGAGGCTGCACCAAGGTCACCCAAGGACCGCGCCCAGCGTCTGCTGGA
GGAGGCCGCTCTGCCCAGCTGGAGGAGCATGGATGGACTGGACGGAACAAACCTGGCCCCACTTTTGCAG
AGCCCCGATGGGAACTGGATGACCTTGAAGGACGGTAGTAGACAGCCCCCAACCCGCCTGCTGACCAAAC
CGAAGAGTGGGACCTTTCAGGCCCTGGAGGTGGCGTCCAGTGTGACGTCTGCCTATGATGAGATCGGTTC
CGACAGTGAGGAAGACTTTGATTACAGCGAAGCCTTGAGTAAGCTGTGCCCACCGTCCCAGAGCAGGCTC
AAGCAACCCCAGCCTCAGCCTACACAGGCCCAGAGTTCTGGCCAAGGTCCCTTAGCCACCTCTCCCTCAA
ACCCTGAGGCCATGTGCTCGGATTCAGAGACATCTTCCACCAGCTCATCTCGGGAAGCTGGCTGCCGGGC
TAAGCTGTCCTGGCTGCAGAGGAAGGCCCCCAAGAACCCCGCAGTGGAGAAGATGCCCCTCCAGGGAGAA
CTGGACGTCAATTTCAACCCTCAGGCAGCCGGTGGAGAGACTTCCGACAGTAGCGATCCAGAGGAGACCC
TCCGCACTGCAGAGCGCCGGGCCAGGAGGTGGAGAAGGGCACGAGTGGGCCCCGAGGAGTCAAACAGGGG
TCTGCCTTCACCTGGTGCCCATCCACGGGCACTGCACACGGCTCAGGTGTCAGACAACGTGTCAGAGACT
GACATCAGCAACGAGACTCAGAATTCCCGGTCCAGCACGGACTCAGTAGAGGAGAAGCTGAGAAATAGAC
TGTATGAGTTAGCCATGAAAATGAGCAAAAAGGAGACTTCTTCTGGGGAGGACCAGGAGTCCGAGTCCAA
GGCGGAACCTAAGAACCAGAAGGGAAGTCTCTCTTCTGAGGAGAACAACCAGGGTGTCCAGGAGGAGCTG
AAGAAGAAGTGTTCTGCTGTTTCTCTCTGCAACATCTCCACAGAAGTCCTGAAGGTCATCAATGCTACCG
AGGAGTTGATCGCAGAGTCTGCAGGGCCCTGGGAGATCCCCCCAGTCTCCACTGACAGAGAAAACGGGAT
GTTTCCTCTTGGGACAGACCAAGTGAGACTGGACAAGCAGCTGACGTCCTTGGAAGAAAACGTGTACCTG
GCAGCGGGCACCGTGTATGGGCTGGAGGGCCAGCTGAGTGAGCTGGAAGACGCTGCCCGCTGCATCCACA
GCAGCACTGGCGAGACGGAGCTGGCAGACCTGGAGGACCAGGTGGCTGCAGCTGCAGCCCAGGTGCACCA
CGCCGAACTCCAGATCTCGGACATCGAGAGCCGCATTTCAGCCCTGACCATCGCTGGCTTAAACATAGCA
CCGTGTGTGCGTCTCACACGAAGACGGGACCAGAAGCAAAGGAGCCAGGTACAAACTATAGACACGTCGA
GGCAGCAGAGGAGGAAACTGCCTGCCCCTCCTGTGAAAGCTGAAAAAATCGAAGCATCTTCCGTGACCCC
CATTAAGACATTCAACCGCAACTTCCTTCTCCAAGGCTCCTCCACAAACAGGCCCACAGCAAGTACCGGT
GACACCAAGGACTTGATGGAGCCTGATCTGGAGTCAGCTGTGATGTAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216068 representing NM_144557
Red=Cloning site Green=Tags(s)

MGRKLDLSGLTDDETEHVLQVVQRDFNLRKKEEDRLSEMKQRLAEENSKCSILSKHQKFVERCCMRCCSP
FTFLVNARRRCGECKFSVCKSCCSYQKHEKLWVCCVCQQARLLRTQSLEWFYNNVKSRFKRFGSAKVLKN
LYRKHRLESGACFDILGGGLFEPNLENEGSISGSDSTFYRQSEGHSMMDTLAVALRVAEEAIEEAISKAE
SHGDSLDKQNEASYLRDHKQELTEELAGTILQRIIRKQKDKAELRAEEEEPEWPRSQSGSVKARGEGTTA
PPGRHKARATFRRSQSAFSFTMEDALKSGSAEAAPRSPKDRAQRLLEEAALPSWRSMDGLDGTNLAPLLQ
SPDGNWMTLKDGSRQPPTRLLTKPKSGTFQALEVASSVTSAYDEIGSDSEEDFDYSEALSKLCPPSQSRL
KQPQPQPTQAQSSGQGPLATSPSNPEAMCSDSETSSTSSSREAGCRAKLSWLQRKAPKNPAVEKMPLQGE
LDVNFNPQAAGGETSDSSDPEETLRTAERRARRWRRARVGPEESNRGLPSPGAHPRALHTAQVSDNVSET
DISNETQNSRSSTDSVEEKLRNRLYELAMKMSKKETSSGEDQESESKAEPKNQKGSLSSEENNQGVQEEL
KKKCSAVSLCNISTEVLKVINATEELIAESAGPWEIPPVSTDRENGMFPLGTDQVRLDKQLTSLEENVYL
AAGTVYGLEGQLSELEDAARCIHSSTGETELADLEDQVAAAAAQVHHAELQISDIESRISALTIAGLNIA
PCVRLTRRRDQKQRSQVQTIDTSRQQRRKLPAPPVKAEKIEASSVTPIKTFNRNFLLQGSSTNRPTASTG
DTKDLMEPDLESAVMY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144557
ORF Size 2568 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144557.5, NP_653140.1
RefSeq Size 4762 bp
RefSeq ORF 2571 bp
Locus ID 245049
UniProt ID Q8K3I4
Cytogenetics 9 F4
Summary Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor proteins MYO5A and MYO7A. May link RAB27A-containing vesicles to actin filaments. Functions as a protein kinase A-anchoring protein (AKAP). May act as a scaffolding protein that links PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_144557" in other vectors (4)
SKU Description Size Price
MC222202 Myrip (untagged) - Mouse myosin VIIA and Rab interacting protein (Myrip), (10ug) 10 ug
$863.00
MR216068 Myrip (Myc-DDK-tagged) - Mouse myosin VIIA and Rab interacting protein (Myrip) 10 ug
$589.00 $785.00
MR216068L3 Lenti ORF clone of Myrip (Myc-DDK-tagged) - Mouse myosin VIIA and Rab interacting protein (Myrip) 10 ug
$1,085.00
MR216068L4 Lenti ORF clone of Myrip (mGFP-tagged) - Mouse myosin VIIA and Rab interacting protein (Myrip) 10 ug
$1,085.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.