Pebp4 (NM_028560) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG216065
Pebp4 (tGFP-tagged) - Mouse phosphatidylethanolamine binding protein 4 (Pebp4) transcript variant 1, (10ug)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pebp4
Synonyms PEBP-4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216065 representing NM_028560
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAATGAAGCTGGTCGCAGCAGCACTTTGCTTGAGCCTCCTTGCAGCCGGCCTGTGGGTGGGCCTCT
CATTGACAGCTGAGTCCATTGAAGAAGGGAAACCTGGAGGAGAGAAACCTGGAGGAGGGAAACCTGGAGG
CAGCGGACGAGGATGTTTTCTCCCACCACTGCCAAAAGAAGATGTTTCACTATGCAGGAACCTAGAAGTT
TTCTACATGGAGATGGGGAACATCAGCTGTAAAATTGTTCCTAAGTGCAATCTATACAGACAGAAGATCA
CGGCCTGGCAGGCGCCGATAGTCAAGTTCCACACGGCTTTGGATGGTGCGCTGTACCTGCTGGTAATGGT
GGATCCAGATGCCCCCAGCAGGTCTAACCCCGTGATGAAATACTGGAGACACTGGTTGGTGTCCAACATC
ACAGGTGCTGACATGAAAAGTGGGAGTATCCGTGGCAATGTACTATCAGACTACTCGCCGCCTACCCCAC
CGCCAGAAACTGGTCTACACCGCTACCAGTTCTTTGTCTACCTTCAGGGAGACAGAGACATTTCTCTCTC
AGTGGAAGAAAAGGCAGACTTAGGTGGTTGGAATCTGGACAAATTCCTGCAGCAATATGGGCTGAGAGAT
CCCGACACAAGCACCCAGTTCATGACTCAGTTTGACGAGGAACTGTCATCAGAGTTTGGTAGAATTAACG
ACGATCAAGAGCAATTCAATCAAAAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216065 representing NM_028560
Red=Cloning site Green=Tags(s)

MTMKLVAAALCLSLLAAGLWVGLSLTAESIEEGKPGGEKPGGGKPGGSGRGCFLPPLPKEDVSLCRNLEV
FYMEMGNISCKIVPKCNLYRQKITAWQAPIVKFHTALDGALYLLVMVDPDAPSRSNPVMKYWRHWLVSNI
TGADMKSGSIRGNVLSDYSPPTPPPETGLHRYQFFVYLQGDRDISLSVEEKADLGGWNLDKFLQQYGLRD
PDTSTQFMTQFDEELSSEFGRINDDQEQFNQK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028560
ORF Size 726 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028560.4, NP_082836.2
RefSeq Size 1047 bp
RefSeq ORF 729 bp
Locus ID 73523
UniProt ID G3X8V3
Cytogenetics 14 D2
Summary Promotes AKT phosphorylation, suggesting a possible role in the PI3K-AKT signaling pathway.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028560" in other vectors (3)
SKU Description Size Price
MR216065 Pebp4 (Myc-DDK-tagged) - Mouse phosphatidylethanolamine binding protein 4 (Pebp4), transcript variant 1 10 ug
$289.00 $300.00
MR216065L3 Lenti ORF clone of Pebp4 (Myc-DDK-tagged) - Mouse phosphatidylethanolamine binding protein 4 (Pebp4), transcript variant 1 10 ug
$600.00
MR216065L4 Lenti ORF clone of Pebp4 (mGFP-tagged) - Mouse phosphatidylethanolamine binding protein 4 (Pebp4), transcript variant 1 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.