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B4galnt2 (NM_008081) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216007
B4galnt2 (tGFP-tagged) - Mouse beta-14-N-acetyl-galactosaminyl transferase 2 (B4galnt2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $676.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol B4galnt2
Synonyms AI593864; Dlb-1; Dlb1; Galg2; Galgt2; Ggm3; Igf2bp1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216007 representing NM_008081
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTTCCAGCGTGTCTTTTGCCAGCTTCAGGTTTCCATGGCTCCTCAAGACATTTGTCCTCATGGTAG
GACTTGCCACTGTTGCGTTTATGGTGAGAAAGGTGTCCCTTACAACAGACTTCAGCACCTTCAAGCCAAA
GTTTCCAGAGCCTGCAAGGGTTGACCCAGTGCTGAAGCTTCTACCAGAGGAGCATCTGAGGAAACTCTTT
ACCTACAGTGACATCTGGCTCTTCCCCAAAAATCAGTGTGACTGTAACTCTGGCAAACTGCGAATGAAAT
ATAAGTTTCAGGATGCCTATAACCAGAAGGACCTTCCAGCTGTGAATGCCAGAAGACAGGCAGAATTTGA
GCACTTTCAGAGGAGAGAAGGGCTGCCTCGCCCACCACCTCTGCTGGCTCCACCCAATCTCCCCTTCGGA
TACCCAGTCCATGGTGTGGAGGTGATGCCCCTGCATACAATTCTCATCCCAGGCCTCCAGTATGAAGGGC
CAGATGCTCCAGTCTATGAGGTCATCCTGAAAGCTTCTCTGGGGACACTGAACACCCTTGCTGATGTGCC
AGATGATGAGGTTCAGGGCAGAGGCCAGAGGCAGCTGACCATTTCCACCAGACATCGGAAGGTCCTGAAT
TTCATCCTTCAGCATGTGACGTACACCAGCACAGAGTACTATCTCCACAAGGTGGACACAGTAAGTATGG
AATACGAGTCGTCAGTGGCCAAGTTTCCAGTGACTATCAAACAACAGACTGTACCCAAGTTGTATGACCC
TGGACCTGAGAGGAAGATCAGAAACCTGGTGACCATTGCCACGAAGACTTTTCTCCGTCCCCACAAGCTT
AAGATCCTGCTTCAGAGTATTCGAAAATATTACCCAGACATTACCGTGATTGTAGCTGATGACAGCAAGG
AGCCCCTGGAAATTAATGATGACTACGTGGAGTACTACACCATGCCCTTTGGGAAGGGCTGGTTTGCTGG
GAGGAACCTGGCCATCTCACAGGTGACTACTAAATATGTCCTCTGGGTGGACGATGACTTTCTCTTCAGC
GACAAGACCAAGATTGAGGTACTGGTGGATGTCCTGGAGAAAACCGAACTGGATGTGGTGGGCGGCAGCG
TGCAGGGGAATACTTACCAGTTCAGGCTGCTGTATGAACAGACCAAGAATGGGAGCTGTCTTCACCAGAG
GTGGGGATCATTCCAGGCCCTTGACGGCTTTCCCGGATGCACGTTGACCAGCGGCGTGGTGAACTTCTTC
TTGGCTCACACGGAACAACTCCGAAGAGTTGGTTTTGATCCCATCTTGCAACGAGTGGCCCACGGAGAGT
TCTTTATTGATGGGCTGGGGAGACTGTTGGTGGGCTCTTGCCCGGGTGTAATTATAAACCACCAAGTAAG
AACACCACCAAAGGATCCAAAGCTGGCTGCCTTGGAGAAGACTTATGACAAATACCGGGCCAACACCAAT
TCTGTGATCCAATTCAAGGTTGCACTCCAGTACTTCAAGAACCATCTCTACTGCTCCACT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216007 representing NM_008081
Red=Cloning site Green=Tags(s)

MTSSVSFASFRFPWLLKTFVLMVGLATVAFMVRKVSLTTDFSTFKPKFPEPARVDPVLKLLPEEHLRKLF
TYSDIWLFPKNQCDCNSGKLRMKYKFQDAYNQKDLPAVNARRQAEFEHFQRREGLPRPPPLLAPPNLPFG
YPVHGVEVMPLHTILIPGLQYEGPDAPVYEVILKASLGTLNTLADVPDDEVQGRGQRQLTISTRHRKVLN
FILQHVTYTSTEYYLHKVDTVSMEYESSVAKFPVTIKQQTVPKLYDPGPERKIRNLVTIATKTFLRPHKL
KILLQSIRKYYPDITVIVADDSKEPLEINDDYVEYYTMPFGKGWFAGRNLAISQVTTKYVLWVDDDFLFS
DKTKIEVLVDVLEKTELDVVGGSVQGNTYQFRLLYEQTKNGSCLHQRWGSFQALDGFPGCTLTSGVVNFF
LAHTEQLRRVGFDPILQRVAHGEFFIDGLGRLLVGSCPGVIINHQVRTPPKDPKLAALEKTYDKYRANTN
SVIQFKVALQYFKNHLYCST

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008081
ORF Size 1530 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008081.1
RefSeq Size 4086 bp
RefSeq ORF 1533 bp
Locus ID 14422
UniProt ID Q09199
Cytogenetics 11 59.02 cM
Summary Responsible for synthesis of murine T-lymphocyte CT antigen. Can transfer N-acetylgalactosamine moiety from UDP-GalNAc to the low molecular weight acceptor 3'-sialyl-N-acetyllactosamine, to form a non-reducing terminal tetrasaccharide Sda blood group structure.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008081" in other vectors (4)
SKU Description Size Price
MC217136 B4galnt2 (untagged) - Mouse beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4galnt2), (10ug) 10 ug
$476.00
MR216007 B4galnt2 (Myc-DDK-tagged) - Mouse beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4galnt2) 10 ug
$289.00 $476.00
MR216007L3 Lenti ORF clone of B4galnt2 (Myc-DDK-tagged) - Mouse beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4galnt2) 10 ug
$776.00
MR216007L4 Lenti ORF clone of B4galnt2 (mGFP-tagged) - Mouse beta-1,4-N-acetyl-galactosaminyl transferase 2 (B4galnt2) 10 ug
$776.00

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