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Tnn (NM_177839) Mouse Tagged ORF Clone

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SKU
MG215968
Tnn (tGFP-tagged) - Mouse tenascin N (Tnn), (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
  $1,668.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Tnn or NM_177839"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tnn
Synonyms tenascin-W; TN-W; Tnw
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215968 representing NM_177839, codon optimized.
Due to the complexity of NM_177839, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGACTGTGGGGAATGCTCGCATTCCCCCTGGGCTTTTTGCTCGCCTCCGTGCTGCTTGTCGCCAGCG
CGCCCGCAACACCAGAATCTCCAGGCTGCTCCAACAAGGAGCAGCAGGTCACTGTGTCCCATACATACAA
GATAGATGTGCCTAAATCTGCCCTCGTGCAGGTTGAGACAGATCCTCAGAGTCTCAGTGACGACGGAACA
AGCCTGCTGGCTCCTGGCGAAGACGGAGAGGAGCAGAACATAATCTTCAGACATAACATCAGACTGCAGA
CCCCACAGAAAAACTGCGATCTTGCGGACAGTGTCCAGGATCTGTTGGCCAGGATGAAAAAGCTGGAGGA
AGAGATGGCTGAGTTGAAGGAGCAATGTAATACCAACAGATGCTGCCAGGGGGCCGCGGACTTGTCCAGA
CATTGCTCTGGCCACGGCACCTTTCTGCCTGAAACATGCTCCTGCCATTGCGACCAAGGCTGGGAGGGAG
CCGATTGCGATCAGCCAACATGCCCCGGGGCTTGCAACGGACACGGACGATGCGTCGATGGACAGTGCGT
GTGCGATGCTCCCTATGTAGGAGTGGACTGCGCTTACGCCGCGTGTCCCCAGGATTGTTCAGGGCATGGA
GTGTGTGTCCAAGGTGTCTGTCAGTGCCACGAGGACTTCACAGCTGAGGACTGCTCCGAGCAGAGGTGCC
CGGGAGATTGCAGTGGGAATGGTTTCTGCGATACTGGTGAGTGCTATTGCGAGATGGGGTTTACCGGGCC
GGACTGCAGTCAGGTGGTAGCCCCACAAGGACTGCAGCTGCTGAAGAGCACCGAGAACTCTCTGCTCGTG
AGCTGGGAGCCCTCCAGTGAGGTAGACTATTACCTCCTCTCTTACTATCCTCTGGGCAAGGAGCAGGCTA
CTAAACAGGTGCGGGTTCCTAAAGAGCAGCATACATACGATATTACAGGGCTGTTGCCCGGGACCAAGTA
TATCGTCACACTTCGAAACGTAAAGAAGGACATCAGCTCAAGTCCCCAGCACCTGCTGGCAACAACCGAT
CTCGCAGTCGTAGGCACCGCCTGGGTTAACGAAGAAACAGAAACTTCTCTTGACGTGGAATGGGAGAACC
CACTTACGGAAGTCGACTATTATAAGCTCAGATATGGGCCCCTGACAGGCCAGGAAGTGACTGAAGTCAC
GGTCCCAAAAAGCAGAGATCCAAAAAGTCGCTATGATATCACAGGCCTGCAGCCAGGGACCGAATATAAA
ATCACTGTGGTGCCCATCCGAGGCGATCTGGAGGGCAAGCCAATACTGTTGAATGGGCGGACCGAAATCG
ATGGCCCGACGAATGTGGTGACAAACCAAGTGACGGAGGATACCGCTTCCGTGTCTTGGGACCCCGTCAG
AGCTGATATCGATAAGTATGTAGTGCGGTACATCGCACCTGATGGGGAAACTAAGGAGAAAGCAGTGCCA
AAAGACCAGAGTAGTACGGTGCTCACTGGACTTAAACCCGGTGAAGCTTATAAAGTGTTTGTCTGGGCCG
AACGAGGCAATCAGGGCTCAAAGAAGGCAGACACAAAGGCATTGACCGAAATTGACTCCCCTGAAAATCT
GGTGACAGATAGGGTCACTGAGAACAGTCTTAGCGTCTCATGGGACCCCGTGGAAGCAGATATCGACCGC
TATGTGGTGAGCTACACTTCCGTCGATGGCGAAACCAAGCAGGTCCCAGTCAAAAAGGATCAGCGATCAA
CCGTGCTGACAGGGTTGTCTCCAGGCGTCGAGTATAAAGTGTACGTGTGGGCTGAGAAGGGTGATAGGGA
GAGCAAGAAGGCCAACACCAAGGCTCCAACAGACATTGATTCTCCCAAGAACCTCGTCACTGACCAGGTC
ACCGAAAACACACTGTCTGTTTCTTGGGATCCTGTACAAGCAAACATCGATAGGTATATGGTGTCATATA
CCAGCGCTGACGGAGAAACACGGGAGGTGCCTGTGCCCAAAGAAAAATCCTCCACCGTCCTGACAGGCCT
TCGCCCAGGCGTGGAGTATAAGGTCCACGTCTGGGCTCAGAAGGGCACTCAGGAATCACGCAAAGCAAAC
ACTAAAGCTCCCACCGATATTGATGGGCCTAAGAACTTGGTGACAGATCAGGTTACTGAAACCACGCTCT
CAGTCAGCTGGGACCCCGTAGAGGCTGATATCGACAGATACATGGTGCGGTATACATCTCCTGACGGTGA
AACAAAGGAAGTGCCAGTTTCCAAAGACAAATCTTCTACAGTTCTCAGGGGGCTGCGCCCTGGCGTCGAA
TACAAGGTCGATGTATGGGCTCAGAAGGGAGCACAGGATAGCAGGAAGGCGAATACTAAGGCGCCCACAG
ATATCGACTCCCCAAAGAATTTGGTGACCGAGCAAGTCACTGAGAGTACAGCAACTGTTAGCTGGGACCC
TGTGGAAGCAGATATTGACCGGTACGTCGTCCGGTACACCTCCGTCGATGGCGAAACGCGAGAATTCTTG
GTGGGCAAAGATCAGACCTCTACGGTTTTGACAGGCATGCGGCCAGGCGTTGAGTACCAAGTCGATGTCT
GGGCACAGAAGGGGACCCAGGAGAGTAGAAAGACTAGCACTAAGGCCCCAACAGACATTGACGGGCCTAA
AAATTTGGTTACTGACCAAGTCACCGAGACTACACTGAGTGTATCTTGGGACCCAGTTGAGGCCGATATC
GATAGGTACATGGTTCGGTACACCTCCCCCGATGGGGAGACGAAAGAGGTGCCAGTTTCCAAGGATAAGT
CAAGCACAGTCCTCCGAGGTTTGAGGCCTGGAGTAGAATACAAGGTTGACGTGTGGGCTCAGAAAGGAGC
ACAAGATTCCCGGAAGGCCAACACCAAAGCACCGACCGATATAGACTCACCCAAAAACCTCGCAATCGAT
CAGGTCACCGAGACAACCCTGTCAGTATCCTGGGATCCTGTGCAGGCTGATATCGATAGGTACGTGGTGA
GATACACGAGCGCTGACGGGGAAAGCAAAGAGTTCTTGATCGGCAAAGAACAGCGAAGTACTGTGCTTAC
GGGACTTAGGCCAGGCGTCGAATATAAGGTCGAGGTCTGGGCCCAGAAGGGGGCCCGGGAGTCCAAGAAA
GCAAATACAGAAGGTCACACTGACATTGACTCTCCTAAGAATCTGGTCACCAATCAGGTGACCGAGAATA
CAGCTACCATTTCTTGGGATCCAGTGCAGGCCGATATTGACCGCTACATGGTGCGGTATACGTCTGCCGA
CGGAGAGACTAGAGAGATCCCGGTCCGAAAAGAGAAGTCATCTACCGTCCTCACAGGTCTGCGACCCGGG
GTCGAGTACACAGTGCAGGTATGGGCCCAGAAAGGCGCCCGCGAATCCAAAAAAGCCAAGACCAAGGCCC
CCACCGAAATTGACTCCCCCAAAAATCTCGTCACTAACCGGGTTACGGAAAATACAGCCACCATAAGTTG
GGACCCTGTGAGGGCTAATATTGATAGGTACATGGTCCGGTATACCTCTGCCGATGGGGAAACAAAGGAG
ATACCCGTCTCAAAGGACCAGAGTTCCACTATACTGACCGGTCTGAAGCCTGGGATGGAGTATACAATCC
ACGTCTGGGCTCAGAAAGGCGCACGGGAGTCTAAGAAAGCCGACACTAAGGCGTTGACCGAGATTGATCC
CCCAAGAAACCTCCGACCTTTCGGGGTAACTCACAGCGGGGGCGTCCTGACATGGCTCCCACCATCCGCC
CAAATCGATGGCTACATTTTGACATACCAGTTTCCTAATGGGACCGTCAAAGAAGTGGAGCTGCCTCGAG
GTCAGCAGCGGTTCGAGCTGCAAGATCTGGAGCAAGGCGTCACATACCCCGTGTCCCTTGTGGCCTTCAA
GGGTAACCAACGGAGCCGAACTGTTAGTACTACACTCTCTACCGTAGATGCCCGGTTTCCACATCCATCT
GACTGTTCACAGGTGCAGCAGAACACAAATGCCGCCTCCGGTCTGTACACCATCTATCTGAACGGGGACG
CCAGTAGGCCTATGCAAGTATACTGCGACATGGATACTGACGGTGGTGGGTGGATCGTTTTTCAGAGAAG
AAATACCGGCCAACTGGACTTTTTTAAAAGATGGAGGAGTTACGTGGAAGGCTTCGGCGACCCTATGAAG
GAGTTTTGGCTGGGACTGGATAAGCTGCACAACCTGACTACAGGAACTACTACAAGGTACGAGGTGAGAG
CCGACCTCCAGACGTTCAACGAAAGTGCTTACGCAGTGTACGATTTTTTTCAGGTTGCATCTAGTAAAGA
GCGGTATAAGTTGTCCGTAGGAAAATACAGAGGTACAGCAGGCGACGCACTGACCTATCACAACGGATGG
AAGTTCACTACTTTTGACAGGGATAACGACATCGCACTCTCCAACTGTGCTCTGACTCACCACGGTGGAT
GGTGGTACAAAAATTGTCACTTGGCTAATCCAAACGGAAAGTATGGGGAGACTAAACATAGCGAGGGCGT
CAACTGGGAACCTTGGAAGGGACACGAATTTTCAATCCCGTATGTGGAGCTCAAAATCAGGCCCTTCGGG
TACAGCCGGGACCGATTCTCTGGGCGCAAGAAAAGGTCAATTGGGAAAGCTCGGATGTTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215968 representing NM_177839
Red=Cloning site Green=Tags(s)

MGLWGMLAFPLGFLLASVLLVASAPATPESPGCSNKEQQVTVSHTYKIDVPKSALVQVETDPQSLSDDGT
SLLAPGEDGEEQNIIFRHNIRLQTPQKNCDLADSVQDLLARMKKLEEEMAELKEQCNTNRCCQGAADLSR
HCSGHGTFLPETCSCHCDQGWEGADCDQPTCPGACNGHGRCVDGQCVCDAPYVGVDCAYAACPQDCSGHG
VCVQGVCQCHEDFTAEDCSEQRCPGDCSGNGFCDTGECYCEMGFTGPDCSQVVAPQGLQLLKSTENSLLV
SWEPSSEVDYYLLSYYPLGKEQATKQVRVPKEQHTYDITGLLPGTKYIVTLRNVKKDISSSPQHLLATTD
LAVVGTAWVNEETETSLDVEWENPLTEVDYYKLRYGPLTGQEVTEVTVPKSRDPKSRYDITGLQPGTEYK
ITVVPIRGDLEGKPILLNGRTEIDGPTNVVTNQVTEDTASVSWDPVRADIDKYVVRYIAPDGETKEKAVP
KDQSSTVLTGLKPGEAYKVFVWAERGNQGSKKADTKALTEIDSPENLVTDRVTENSLSVSWDPVEADIDR
YVVSYTSVDGETKQVPVKKDQRSTVLTGLSPGVEYKVYVWAEKGDRESKKANTKAPTDIDSPKNLVTDQV
TENTLSVSWDPVQANIDRYMVSYTSADGETREVPVPKEKSSTVLTGLRPGVEYKVHVWAQKGTQESRKAN
TKAPTDIDGPKNLVTDQVTETTLSVSWDPVEADIDRYMVRYTSPDGETKEVPVSKDKSSTVLRGLRPGVE
YKVDVWAQKGAQDSRKANTKAPTDIDSPKNLVTEQVTESTATVSWDPVEADIDRYVVRYTSVDGETREFL
VGKDQTSTVLTGMRPGVEYQVDVWAQKGTQESRKTSTKAPTDIDGPKNLVTDQVTETTLSVSWDPVEADI
DRYMVRYTSPDGETKEVPVSKDKSSTVLRGLRPGVEYKVDVWAQKGAQDSRKANTKAPTDIDSPKNLAID
QVTETTLSVSWDPVQADIDRYVVRYTSADGESKEFLIGKEQRSTVLTGLRPGVEYKVEVWAQKGARESKK
ANTEGHTDIDSPKNLVTNQVTENTATISWDPVQADIDRYMVRYTSADGETREIPVRKEKSSTVLTGLRPG
VEYTVQVWAQKGARESKKAKTKAPTEIDSPKNLVTNRVTENTATISWDPVRANIDRYMVRYTSADGETKE
IPVSKDQSSTILTGLKPGMEYTIHVWAQKGARESKKADTKALTEIDPPRNLRPFGVTHSGGVLTWLPPSA
QIDGYILTYQFPNGTVKEVELPRGQQRFELQDLEQGVTYPVSLVAFKGNQRSRTVSTTLSTVDARFPHPS
DCSQVQQNTNAASGLYTIYLNGDASRPMQVYCDMDTDGGGWIVFQRRNTGQLDFFKRWRSYVEGFGDPMK
EFWLGLDKLHNLTTGTTTRYEVRADLQTFNESAYAVYDFFQVASSKERYKLSVGKYRGTAGDALTYHNGW
KFTTFDRDNDIALSNCALTHHGGWWYKNCHLANPNGKYGETKHSEGVNWEPWKGHEFSIPYVELKIRPFG
YSRDRFSGRKKRSIGKARMF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177839
ORF Size 4680 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177839.3, NP_808507.2
RefSeq Size 5825 bp
RefSeq ORF 4683 bp
Locus ID 329278
UniProt ID Q80Z71
Cytogenetics 1 H2.1
Summary Extracellular matrix protein that seems to be a ligand for ITGA8:ITGB1, ITGAV:ITGB1 and ITGA4:ITGB1 (By similarity) (PubMed:14709716). Involved in neurite outgrowth and cell migration in hippocampal explants (PubMed:12812753). During endochondral bone formation, inhibits proliferation and differentiation of proteoblasts mediated by canonical WNT signaling (PubMed:17395156). In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (By similarity). Expressed in most mammary tumors, may facilitate tumorigenesis by supporting the migratory behavior of breast cancer cells (PubMed:15592496).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_177839" in other vectors (2)
SKU Description Size Price
MC224688 Tnn (untagged) - Mouse tenascin N (Tnn), (10ug) 10 ug
$1,469.00
MR215968 Tnn (Myc-DDK-tagged) - Mouse tenascin N (Tnn). Note: ORF is codon optimized 10 ug
$1,468.00

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