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Dusp7 (NM_153459) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215943
Dusp7 (tGFP-tagged) - Mouse dual specificity phosphatase 7 (Dusp7), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Dusp7 or NM_153459"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dusp7
Synonyms AU015694; Mkpx; Pyst2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215943 representing NM_153459
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAAACCAGCTCCGCGGGCCCCCAGTGCGGGCGCACATGTCGACTTCGGGGGCGGCGGCGGCGGCGG
CGGCTGGGGGCACTCGGGCGGGGTCGGAGCCCGGTGCGGGATCTGGGTCCGGCGCAGGCATCGGAGCGGG
AGCGACGACGGGAGCGGGGGCCATGCCCTGCAAGAGCGCCGAGTGGCTGCAGGAGGAGCTGGAGGCGCGC
GGTGGCGCGTCCCTGCTGCTGCTAGATTGCCGGCCGCACGAGCTCTTCGAATCGTCGCACATTGAGACGG
CCATCAACCTGGCCATCCCGGGTCTCATGCTGCGCCGCCTGCGCAAAGGCAACCTGCCCATCCGCTCCAT
CATTCCCAACCATGCCGACAAGGAGCGCTTCGCCACGCGCTGCAAGGCGGCCACCGTGCTGCTCTACGAC
GAGGCCACGGCGGAGTGGCAGCCCGAGCCCGGCGCCCCCGCCTCCGTTCTCGGCCTGCTCCTGCAGAAGC
TGCGAGACGACGGCTGCCAGGCCTACTACCTCCAAGGTGGTTTCAACAAGTTCCAGACGGAATACTCCGA
GCATTGTGAGACTAACGTGGACAGCTCGTCCTCCCCGAGTGGCTCGCCACCCACCTCCGTGCTGGGCCTG
GGGGGCCTGCGCATCAGCTCTGACTGCTCAGACGGCGAGTCGGACCGAGAGCTGCCCAGCAGTGCCACTG
AGTCGGATGGCAGCCCTGTACCATCCAGCCAGCCGGCCTTTCCAGTCCAGATCCTGCCCTACCTCTACCT
CGGCTGTGCCAAGGACTCTACCAACCTGGATGTACTTGGCAAATACGGCATCAAATACATCCTCAACGTC
ACACCCAACCTGCCCAACGCCTTTGAGCACGGTGGTGAGTTCACCTACAAGCAAATCCCCATCTCTGACC
ACTGGAGCCAGAACCTCTCTCAGTTCTTCCCCGAGGCCATCAGCTTCATTGATGAAGCCCGCTCCAAGAA
GTGTGGTGTCTTGGTGCACTGCCTAGCAGGCATCAGCCGTTCAGTGACAGTTACTGTAGCCTACCTGATG
CAGAAGATGAACCTGTCCCTCAACGACGCCTATGACTTCGTCAAGAGGAAAAAGTCTAACATCTCGCCCA
ACTTCAACTTCATGGGGCAGCTGCTGGACTTTGAGCGGACGCTGGGGCTCAGCAGCCCATGCGACAACCA
CGCACCCAGTGAGCAGCTCTACTTCTCCACGCCCACCAACCATAACCTGTTCCCGATCAACACGCTCGAG
TCCACG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215943 representing NM_153459
Red=Cloning site Green=Tags(s)

MKNQLRGPPVRAHMSTSGAAAAAAAGGTRAGSEPGAGSGSGAGIGAGATTGAGAMPCKSAEWLQEELEAR
GGASLLLLDCRPHELFESSHIETAINLAIPGLMLRRLRKGNLPIRSIIPNHADKERFATRCKAATVLLYD
EATAEWQPEPGAPASVLGLLLQKLRDDGCQAYYLQGGFNKFQTEYSEHCETNVDSSSSPSGSPPTSVLGL
GGLRISSDCSDGESDRELPSSATESDGSPVPSSQPAFPVQILPYLYLGCAKDSTNLDVLGKYGIKYILNV
TPNLPNAFEHGGEFTYKQIPISDHWSQNLSQFFPEAISFIDEARSKKCGVLVHCLAGISRSVTVTVAYLM
QKMNLSLNDAYDFVKRKKSNISPNFNFMGQLLDFERTLGLSSPCDNHAPSEQLYFSTPTNHNLFPINTLE
ST

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153459
ORF Size 1266 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153459.4, NP_703189.3
RefSeq Size 3227 bp
RefSeq ORF 1269 bp
Locus ID 235584
UniProt ID Q91Z46
Cytogenetics 9 F1
Summary Dual specificity protein phosphatase (PubMed:27783954). Shows high activity towards MAPK1/ERK2 (By similarity). Also has lower activity towards MAPK14 and MAPK8 (By similarity). In arrested oocytes, plays a role in meiotic resumption (PubMed:27783954). Promotes nuclear envelope breakdown and activation of the CDK1/Cyclin-B complex in oocytes, probably by dephosphorylating and inactivating the conventional protein kinase C (cPKC) isozyme PRKCB (PubMed:27783954). May also inactivate PRKCA and/or PRKCG (PubMed:27783954). Also important in oocytes for normal chromosome alignment on the metaphase plate and progression to anaphase, where it might regulate activity of the spindle-assembly checkpoint (SAC) complex (PubMed:27783954).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_153459" in other vectors (4)
SKU Description Size Price
MC213009 Dusp7 (untagged) - Mouse dual specificity phosphatase 7 (Dusp7), (10ug) 10 ug
$457.00
MR215943 Dusp7 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 7 (Dusp7) 10 ug
$289.00 $457.00
MR215943L3 Lenti ORF clone of Dusp7 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 7 (Dusp7) 10 ug
$757.00
MR215943L4 Lenti ORF clone of Dusp7 (mGFP-tagged) - Mouse dual specificity phosphatase 7 (Dusp7) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.