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Prpf40b (NM_018786) Mouse Tagged ORF Clone

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SKU
MG215937
Prpf40b (tGFP-tagged) - Mouse PRP40 pre-mRNA processing factor 40 homolog B (yeast) (Prpf40b), (10ug)
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ORF Plasmid:
tGFP DDK
  $999.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Prpf40b
Synonyms 2610317D23Rik; Hypc
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215937 representing NM_018786
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCCACCACCCTTCATGCCCCCTCCAGGGCTCCCACCTCCTTTTCCTCCGATGGGGCTCCCCCCTA
TGAGTCAGAGACCACCAGCCATTCCCCCCATGCCACCTGGCATACTGCCCCCAATGCTTCCACCAATGGG
GGCACCGCCACCACTTACACAGATACCAGGAATGGTCCCTCCAATGATGCCGGGGATGCTGATGCCAGCG
GTGCCTGTCACTGCAGCGACGGCTCCGGGTGCGGACACCGCCAGCTCTGCTGTGGCTGGAACAGGCCCTC
CGAGGGCCTTATGGAGTGAGCACGTAGCCCCTGACGGGCGCATCTACTACTACAATGCTGATGATAAGCA
ATCCGTGTGGGAGAAGCCCAGCGTACTCAAGTCTAAGGCAGAGCTGCTGCTGTCCCAGTGTCCCTGGAAA
GAGTATAAGTCAGATACAGGGAAGCCTTACTACTACAACAACCAGAGTCAGGAGTCCCGGTGGACTCGGC
CCAAGGACCTGGATGACCTGGAAGCCCTCGTCAAACAAGAGTCTGCAGGAAAACAGCAGACCCAGCAACT
ACAGACACTACAGCCACAACCACCTCAGCCACAGCCTGACCCTCCACCTATACCTCCGGGTCCCATCCCA
GTGCCTATGGCCCTCCTGGAACCTGAACCAGGTCGAAGTGAAGACTGTGATGTGCTGGAGGCTGCCCAGC
CCCTGGAGCAGGGCTTCCTGCAGCGGGAGGAGGGCCCCAGCAGCTCTACTGGACAGCATCGGCAGCCACA
GGAAGAGGAGGAAGCTAAGCCAGAACCAGAGCGATCTGGCCTCAGTTGGAGCAACCGGGAGAAGGCAAAA
CAGGCTTTCAAAGAACTGCTAAGGGACAAGGCTGTCCCTTCCAATGCTTCATGGGAACAGGCCATGAAGA
TGGTAGTCACTGACCCCCGCTACAGTGCCTTGCCCAAACTAAGTGAGAAGAAGCAGGCGTTTAATGCTTA
CAAGGCTCAGCGGGAGAAGGAGGAGAAAGAGGAGGCCCGGCTGAGAGCCAAGGAGGCCAAGCAGACCCTG
CAGCATTTCCTGGAACAGCATGAACGCATGACCTCCACCACCCGCTACCGGCGGGCAGAACAGACTTTTG
GGGACCTGGAGGTCTGGGCTGTGGTCCCTGAGAGAGAGAGAAAGGAAGTTTATGATGATGTCCTCTTCTT
CCTGGCCAAGAAGGAGAAGGAACAAGCCAAGCAGCTCCGGCGTCGGAATATCCAGGCCCTGAAGAGCATC
CTGGATGGGATGAGCAGTGTCAACTTCCAAACCACTTGGTCCCAGGCCCAGCAGTACCTCATGGATAACC
CCAGCTTTGCTCAGGACCAGCAGCTGCAGAACATGGACAAGGAAGATGCACTTATCTGCTTTGAGGAGCA
TATCCGGGCTTTGGAGAGAGAGGAGGAGGAAGAGCGAGAACGGGCCCGGCTTCGGGAGCGGCGACAGCAG
CGGAAGAATCGGGAGGCCTTTCAGAGCTTCCTGGACGAGCTGCATGAGACAGGGCAGCTGCACTCCATGT
CCACCTGGATGGAGCTGTACCCGGCAGTCAGCACTGACGTTCGCTTTGCCAACATGCTGGGCCAGCCGGG
CTCTACTCCTTTGGACTTGTTCAAGTTCTATGTGGAGGAGTTGAAGGCTCGATTCCACGATGAGAAGAAG
ATCATAAAGGATATCCTTAAGGATCGGGGCTTCTGTGTGGAGGTGAACACAGCATTTGAGGACTTCGCCC
ACGTCATAAGCTTTGACAAGAGGGCTGCTGCGCTGGACGCAGGCAACATCAAGCTGACCTTCAATAGTCT
GCTGGAAAAAGCAGAGGCACGGGAGAGGGAACGGGAAAAGGAGGAGGCCCGAAGGATGCGGCGCAGAGAA
GCTGCCTTTCGAAGCATGCTGAGGCAGGCCGTGCCTGCTCTGGAGCTGGGCACTGCCTGGGAAGAGGTCC
GTGAGCGCTTTGTGTGCGACTCAGCCTTTGAGCAGATCACCCTGGAGTCGGAGCGGATCCGGCTCTTCCG
AGAGTTCCTGCAGGTGTTGGAGACTGAATGCCAGCACCTCCACACCAAAGGCCGAAAGCATGGCAGAAAG
GGCAAGAAACACCATCGCAAGCGTTCACACTCACCCTCAGTGAGTCGGCAGGGCTCTGAGTCAGATGAAG
AGGAGCTGCCCCCACCATCCCTCCGGCCCCCCAAGCGGAGGAGGCGGAACCCCTCAGAATCTGGCTCTGA
GCCCTCCTCCTCACTTGATTCAGTTGAAAGTGGGGGTGCTGCTCTTGGAGGACCAGGCTCCCCATCTTCC
CACCTTCTTCTTGGGTCAGATCATGGTCTTCGGAAAACCAAGAAACCAAAAAAGAAAACCAAGAAAAGAA
GACACAAGTCGACCAGTCCTGACAGTGAGACGGACCCCGAAGACAAAGCTGGTAAGGAGAGTGAAGACAG
AGAACAGGAACAGGACAGGGAACCCCGGCAGGCAGAGCTCCCTAACCGTTCCCCAGGCTTCGGAATCAAG
AAGGAGAAGACAGGCTGGGACACGTCAGAAAGCGAGCTGAGTGAGGGGGAGCTGGAGAGGCGAAGGCGGA
CACTCCTACAGCAGCTGGACGACCACCAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215937 representing NM_018786
Red=Cloning site Green=Tags(s)

MMPPPFMPPPGLPPPFPPMGLPPMSQRPPAIPPMPPGILPPMLPPMGAPPPLTQIPGMVPPMMPGMLMPA
VPVTAATAPGADTASSAVAGTGPPRALWSEHVAPDGRIYYYNADDKQSVWEKPSVLKSKAELLLSQCPWK
EYKSDTGKPYYYNNQSQESRWTRPKDLDDLEALVKQESAGKQQTQQLQTLQPQPPQPQPDPPPIPPGPIP
VPMALLEPEPGRSEDCDVLEAAQPLEQGFLQREEGPSSSTGQHRQPQEEEEAKPEPERSGLSWSNREKAK
QAFKELLRDKAVPSNASWEQAMKMVVTDPRYSALPKLSEKKQAFNAYKAQREKEEKEEARLRAKEAKQTL
QHFLEQHERMTSTTRYRRAEQTFGDLEVWAVVPERERKEVYDDVLFFLAKKEKEQAKQLRRRNIQALKSI
LDGMSSVNFQTTWSQAQQYLMDNPSFAQDQQLQNMDKEDALICFEEHIRALEREEEEERERARLRERRQQ
RKNREAFQSFLDELHETGQLHSMSTWMELYPAVSTDVRFANMLGQPGSTPLDLFKFYVEELKARFHDEKK
IIKDILKDRGFCVEVNTAFEDFAHVISFDKRAAALDAGNIKLTFNSLLEKAEAREREREKEEARRMRRRE
AAFRSMLRQAVPALELGTAWEEVRERFVCDSAFEQITLESERIRLFREFLQVLETECQHLHTKGRKHGRK
GKKHHRKRSHSPSVSRQGSESDEEELPPPSLRPPKRRRRNPSESGSEPSSSLDSVESGGAALGGPGSPSS
HLLLGSDHGLRKTKKPKKKTKKRRHKSTSPDSETDPEDKAGKESEDREQEQDREPRQAELPNRSPGFGIK
KEKTGWDTSESELSEGELERRRRTLLQQLDDHQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018786
ORF Size 2619 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018786.3
RefSeq Size 3182 bp
RefSeq ORF 2622 bp
Locus ID 54614
UniProt ID Q80W14
Cytogenetics 15 F1
Summary May be involved in pre-mRNA splicing.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_018786" in other vectors (4)
SKU Description Size Price
MC222325 Prpf40b (untagged) - Mouse PRP40 pre-mRNA processing factor 40 homolog B (yeast) (Prpf40b), (10ug) 10 ug
$880.00
MR215937 Prpf40b (Myc-DDK-tagged) - Mouse PRP40 pre-mRNA processing factor 40 homolog B (yeast) (Prpf40b) 10 ug
$589.00 $799.00
MR215937L3 Lenti ORF clone of Prpf40b (Myc-DDK-tagged) - Mouse PRP40 pre-mRNA processing factor 40 homolog B (yeast) (Prpf40b) 10 ug
$1,099.00
MR215937L4 Lenti ORF clone of Prpf40b (mGFP-tagged) - Mouse PRP40 pre-mRNA processing factor 40 homolog B (yeast) (Prpf40b) 10 ug
$1,099.00

file-alt Citations

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