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Hectd2 (NM_172637) Mouse Tagged ORF Clone

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SKU
MG215928
Hectd2 (tGFP-tagged) - Mouse HECT domain containing 2 (Hectd2) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $911.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hectd2
Synonyms 4921524L07; A630025O09Rik; AW212605
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215928 representing NM_172637
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGAGGCGGCTCGGGATCTCTCGCCCGGAGCGCCGCCGGCGGTAGCCGCGGCCGCCCCGGAGGAGA
GGAAGGGGAAAGAACCGGAGCGCGAGAAGCTGCCGCCCATCGTGACGGCCGGCGCAGCCGCGGGCTTGGA
CAGAGGATCCAAGGGCCAGATTTCCACCTTCAGCAGCTTTGTCTCGACTGTCACCCAGAAGAAGGAAGCT
GCTGAGAACAGAAGCTCGCCTACCCACCTTGCTCTCCCTAACATCAGGAATGTGAGAGACCTACCACCAA
TTTGTCTTGATGTTAGACAAAAACAGCGCATGTCTGTGGAAGCGTTACCATCTGAAGTGAAGGTCCCGCC
CCTTCCAGAACCTTCTCTTCCTAGCCAGCCCAAAACTGTAAAAGACTTTGAGGAAGATTTAGAAAAAGCT
GAGGCCACAGGGAATTGGAAGACAGTACATGCTTTTTATATAACAGCATTTGATTCTTTCACAGAACTAA
ACACTGCATTTAAGAAAGATGCCACGGCCTCATTTAATACTATTGAGGACTCTGGGCTTAATGCCAATTT
GGTGAATGCTGTGTTTGATGCCTTACTTAATACTCCTCAAGATATCCAGAAGTCAGTATTGAAGGGAATC
ATTAACAGCTTGTTACAAGAATGGAAAGGTCCACGAACAAAAGATGATCTTAGAGCATATTTTATACTGT
TACAGAATCCTCAATTTAATATCACATCTACATATGTCATCTATGCTCATTTGCTACGACAGATAGCTAC
CTTAGTGGAAGCTGACCATCACTTCCTAGTTCACTGGCTTAAAAAATTATCCCAGAAGAAATTCAAGCAA
CTGGTAGAGAGATTGCTGCAATTTGTTTCTTTACGGCTGTTTCCAGCAAAACCTGAAGAATTCCCGCCTC
TAACGAAGTGTACCTGGTGGATCCCATCAGCCGCTAAAGTTCTGGCTTTGCTTAACACTGCCAACAATTT
GGTTCACCCTCCCCTCGTTCCTTACACTGATTTCTATAATTCTACCCTGGATCACATTGATCTCATGGAA
GAGTATCACACGTGGCAGAGCTTTGGGAACTCTCACAGCAGGTTTTCCTTCTGTCAGTACCCATTCGTTA
TTTCTATAGCTGCAAAAAAAATCATTATTCAAAGAGACTCAGAACAACAGATGATAAGCATCGCAAGGCA
AAGTCTGGTGGATAAAGTATCTCGAAGACAAAGACCTGATATGAATATGTTATTTCTAAATATGAAAGTA
AGGAGGACACATCTGGTTAGCGACTCACTTGATGAGTTAACCCGGAAGAGAGCTGACTTGAAGAAGAAGT
TGAAAGTTACATTTGTAGGTGAAGCTGGTTTGGATATGGGCGGCTTGACTAAAGAATGGTTCCTTCTTCT
AATTCGCCAGATTTTCCATCCAGATTATGGCATGTTTACATACCACAAGGATTCACACTGCCATTGGTTT
AGCAGCTTTAAATGTGATAACTATTCTGAATTCCGATTGGTTGGAATTCTCATGGGACTAGCTGTTTATA
ACAGCATCACCTTGGATATTCGTTTTCCTCCCTGCTGTTACAAAAAATTACTGAGCCCTCCTGTCGTACC
AAGTGATCAGAGTACACCAGTAGGCATCTGCAGTGTTACCATTGACGACTTGTGCCAGGTTATGCCTGAA
TTGGCCCATGGACTAAAGGAACTTTTATCATATGAAGGCAACGTTGAAGAAGATTTCTATTCAACATTTC
AGGTTTTTCAAGAAGAATTTGGAGTAATTAAATCCTATAACTTAAAACCAGGGGGTGATAAGATTCCAGT
TACCAATCAGAATAGAAGAGAGTATGTACAGCTTTATACTGACTTCCTGTTGAACAAATCTATCTATAAG
CAATTTGCTGCATTTTACTGTGGATTTCATAGTGTGTGTGCTTCAAATGCCCTAATGCTGCTTCGTCCAG
AAGAAGTAGAAATCCTGGTTTGTGGCAGTCCTGAACTGGATATGCATGCACTGCAGAGGAGCACACAGTA
CGACGGCTATGCGAAGACAGACCTGACCATACGATACTTTTGGGATGTTGTGCTTGGATTTCCACTTGAA
CTTCAGAAAAAGCTGTTACACTTTACTACAGGAAGCGACAGAGTACCTGTTGGAGGGATGGCTGATTTGA
ATTTTAAAATTTCAAAGAATGAAACTTCTACTAACTGGTTACCTGTGGCACACACTTGTTTCAATCAACT
CTGCCTTCCCCCATACAAGAGCAAAAAAGATCTGAAACAGAAACTGATTATTGGAATTTCAAATTCAGAA
GGTTTTGGACTTGAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215928 representing NM_172637
Red=Cloning site Green=Tags(s)

MSEAARDLSPGAPPAVAAAAPEERKGKEPEREKLPPIVTAGAAAGLDRGSKGQISTFSSFVSTVTQKKEA
AENRSSPTHLALPNIRNVRDLPPICLDVRQKQRMSVEALPSEVKVPPLPEPSLPSQPKTVKDFEEDLEKA
EATGNWKTVHAFYITAFDSFTELNTAFKKDATASFNTIEDSGLNANLVNAVFDALLNTPQDIQKSVLKGI
INSLLQEWKGPRTKDDLRAYFILLQNPQFNITSTYVIYAHLLRQIATLVEADHHFLVHWLKKLSQKKFKQ
LVERLLQFVSLRLFPAKPEEFPPLTKCTWWIPSAAKVLALLNTANNLVHPPLVPYTDFYNSTLDHIDLME
EYHTWQSFGNSHSRFSFCQYPFVISIAAKKIIIQRDSEQQMISIARQSLVDKVSRRQRPDMNMLFLNMKV
RRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSHCHWF
SSFKCDNYSEFRLVGILMGLAVYNSITLDIRFPPCCYKKLLSPPVVPSDQSTPVGICSVTIDDLCQVMPE
LAHGLKELLSYEGNVEEDFYSTFQVFQEEFGVIKSYNLKPGGDKIPVTNQNRREYVQLYTDFLLNKSIYK
QFAAFYCGFHSVCASNALMLLRPEEVEILVCGSPELDMHALQRSTQYDGYAKTDLTIRYFWDVVLGFPLE
LQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNWLPVAHTCFNQLCLPPYKSKKDLKQKLIIGISNSE
GFGLE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172637
ORF Size 2325 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172637.3, NP_766225.2
RefSeq Size 4771 bp
RefSeq ORF 2325 bp
Locus ID 226098
UniProt ID Q8CDU6
Cytogenetics 19 C2
Summary Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172637" in other vectors (4)
SKU Description Size Price
MC221524 Hectd2 (untagged) - Mouse HECT domain containing 2 (Hectd2), transcript variant 2, (10ug) 10 ug
$781.00
MR215928 Hectd2 (Myc-DDK-tagged) - Mouse HECT domain containing 2 (Hectd2), transcript variant 2 10 ug
$589.00 $711.00
MR215928L3 Lenti ORF clone of Hectd2 (Myc-DDK-tagged) - Mouse HECT domain containing 2 (Hectd2), transcript variant 2 10 ug
$1,011.00
MR215928L4 Lenti ORF clone of Hectd2 (mGFP-tagged) - Mouse HECT domain containing 2 (Hectd2), transcript variant 2 10 ug
$1,011.00

file-alt Citations

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