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Chp2 (NM_027363) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215924
Chp2 (tGFP-tagged) - Mouse RIKEN cDNA 2010110P09 gene (2010110P09Rik), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Chp2 or NM_027363"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Chp2
Synonyms 2010110P09Rik; Cbhp2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215924 representing NM_027363
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTCACGAAGCTCTCATATCGCGCTCATTCCAGACGTGGAGCACATTCGCAGAGAGACAGGCTTCT
CGCAAGCCAGCCTGCTCCGCCTCTACCACCGGTTCCAAGCCCTGGACAGGGATGAAAAGGGCTTCCTAAG
CCGCCTAGACCTCCAGCAGATCGGAGCACTGGCTGTGAATCCACTGGGTGACCGCATTATAGACAGCTTC
TTCCCCAACGGAAGTCAGAGACTGTATTTCGCAGGCTTTGCCAGGGTCCTGGCTTATTTTCGACCTATAG
ATGAAGAAGATGCCACACTCCGAGACCCCAAGCAACCTGAACCCCTAAACAGCAGAATGAACAAACTGCG
CTTTGCATTTCAGCTCTATGATCTGGATCGGGATGGGAAGATCTCCAGGAATGAAATGCTACAGGTTCTC
CGCCTCATGGTTGGGGTCCAGGTGACGGATGAGCAGCTGGAGAGCATCACAGACCGTACAGTGCAGGAAG
CTGATGAGGACGGGGATGGGGCTGTGTCCTTCCTGGAGTTCACCAAGTCCTTAGAGAAGATGAACATCGA
ACAAAAAATGAGCATCCGGATCCTGAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215924 representing NM_027363
Red=Cloning site Green=Tags(s)

MGSRSSHIALIPDVEHIRRETGFSQASLLRLYHRFQALDRDEKGFLSRLDLQQIGALAVNPLGDRIIDSF
FPNGSQRLYFAGFARVLAYFRPIDEEDATLRDPKQPEPLNSRMNKLRFAFQLYDLDRDGKISRNEMLQVL
RLMVGVQVTDEQLESITDRTVQEADEDGDGAVSFLEFTKSLEKMNIEQKMSIRILK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027363
ORF Size 588 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027363.1, NP_081639.1
RefSeq Size 1171 bp
RefSeq ORF 591 bp
Locus ID 70261
UniProt ID Q9D869
Cytogenetics 7 F2
Summary Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027363" in other vectors (4)
SKU Description Size Price
MC211048 Chp2 (untagged) - Mouse RIKEN cDNA 2010110P09 gene (2010110P09Rik), (10ug) 10 ug
$330.00
MR215924 Chp2 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2010110P09 gene (2010110P09Rik) 10 ug
$289.00 $300.00
MR215924L3 Lenti ORF clone of Chp2 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2010110P09 gene (2010110P09Rik) 10 ug
$600.00
MR215924L4 Lenti ORF clone of Chp2 (mGFP-tagged) - Mouse RIKEN cDNA 2010110P09 gene (2010110P09Rik) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.