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Carmil1 (NM_026825) Mouse Tagged ORF Clone

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SKU
MG215923
Lrrc16a (tGFP-tagged) - Mouse leucine rich repeat containing 16A (Lrrc16a), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,375.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Carmil1 or NM_026825"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Carmil1
Synonyms 1110037D04Rik; AI425970; CARMIL; CARML1; D130057M20; Lrrc16; Lrrc16a
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215923 representing NM_026825
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGACGAGAGCTCCGATGTCCCCCGGGAGTTAATGGAAAGCATCAAGGATGTTATTGGCAGGAAGA
TCAAAATCTCAGTGAAGAAGAAAGTGAAGTTGGAAGTGAAGGGCGACAGGGTGGAAAACAAAGTGCTGGT
GCTCACATCATGCCGAGCCTTCCTCCTGTCAGCACGCATCCCCAGCAAGCTCGAGTTAACCTTCAGTTAT
CTAGAGATTCATGGAGTCATCTGCCACAAGCCAGCCCAGATGGTTGTGGAGACTGAGAAGTGTAACATGT
CCATGAAGATGGTGTCCCCAGAGGATGTGAGTGAAGTGCTGGCTCACATAGGCACCTGCTTGCGGAGGAT
ATTCCCAGGCCTTTCCCCACTGAGAATCATGAAGAAAGTCTCCATGGAGCCATCTGAACGTCTGGCCAGT
CTCCAGGCCCTGTGGGACAGCCAGACCTTGGCTGAGCCAGGCCCCTGTGGTGGGTTTTCTCAGATGTATG
CCTGTGTGTGCGACTGGCTGGGCTTTTCATACAAGGAAGAAGTGCAGTGGGATGTGGATACAATTTATCT
GACACAAGACACCAGGGAATTAAATTTACAGGACTTCAGTCATCTCGAGCACAGAGACCTGATCCCCATC
ATTGCTGCCCTGGAGTATAACCAGTGGTTCACCAAACTGTCCTCTAAGGATCTCAAACTGTCCACGGATG
TTTGTGAACAGATTTTGAGAGTGGTGAGTAGGTCCAATCGACTGGAAGAGTTGGTGTTGGAAAATGCTGG
ACTCAGAATAGATTTTGCACAGAAATTGGCCGGTGCTCTAGCACACAATCCTAACTCAGGACTTCACACA
ATCAACCTTGCTGGGAACTCGCTGGAGGATCGAGGTGTGTCCTCCTTAAGTATTCAATTTGCCAAACTCC
CAAAGGGATTGAAGCATTTGAACCTCTCTAAAACCTCATTGTCACCTAAAGGGGTGAACAGCCTTTGTCA
GTCCCTCAGTGCCAACCCTCTGACTGCCTCTACCCTCACCCACCTGGACCTCTCAGGGAATGCCCTCCGC
GGAGATGACCTCTCGCACATGTACAATTTTTTGGCCCAGCCCAACACCATTGTTCATCTGGATCTATCTA
ATACAGAATGTTCTCTGGAGATGGTGTGTAGCGCTCTCCTCCGGGGTTGCCTTCAGTGTCTCGCTGTGCT
CAATCTCTCCAGATCTGTCTTCTCTCACAGGAAAGGAAAGGAAGTCCCTCCGTCTTTCAAGCAGTTTTTC
AGCAGTTCTCTGGCTTTGATCCAAATCAACCTTTCAGGCACGAAGCTGTCTCCCGAGCCCTTGAAAGCGC
TGCTCTTGGGCCTGGCCTGCAATCATAGCCTGAAGGGAGTCTCTCTGGATCTCAGCAACTGTGAGCTTGG
CCACTGTCTGAGGTCAGGAGGAGCTCAAGTGTTAGAAGGCTGCATCGCTGAAATCCACAACATCACCAGC
TTAGATATCTCTGACAATGGTTTAGAATCCGACCTATCTACCCTAATTGTGTGGCTCAGTAAAAACAGAT
CAATACAGCACCTGGCATTGGGCAAAAATTTTAATAATATGAAATCCAAAAATCTGACGCCTGTGTTGGA
CAACTTAGTGCAGATGATCCAAGATGAAGACTCTCCTCTGCAGTCGTTGTCCCTGGCTGACTCAAAACTC
AAGGCTGAGGTCACCATCATCATCAATGCCCTGGGCAGCAACACCTCCCTGACCAAAGTGGACATCAGTG
GGAATGGCATGGGGGACATGGGAGCGAAGATGCTGGCCAAGGCTCTCCAGATCAACACCAAACTCAGGAC
AGTCATCTGGGACAAAAACAACATCACTGCACAAGGCTTTCAAGACATAGCTGTTGCTATGGAAAAGAAC
TACACACTGCGATTTATGCCAATTCCGATGTATGATGCCGCTCAAGCTCTCAAAACCAACCCCGAGAAAA
CAGAAGAGGCTCTGCAGAAGATAGAAAATTATCTGCTCCGGAATCACGAGACCAGGAAATACCTTCAGGA
GCAGGCCTATCGCCTCCAGCAGGGCATCGTCACCAGCACCACCCAGCAGATGATTGACCGGATATGTGTG
AAGGTTCAGGATCACCTCAACTCCTTACGAGCCTGTGGGGGTGATGCTATTCAGGAGGATTTAAAGGCAG
CGGAGCGGCTCATGCGAGATGCTAAGAACTCTAAAACATTGTTGCCCAATTTATATCATGTGGGCGGTGC
ATCCTGGGCAGGAGCCAGTGGGCTGTCATCCAGTCCCATTCAGGAGACCCTGGAGTCGATGGCTGGAGAA
GTCACAAGAGTGGTGGATGAACAACTGAAGGATTTGCTAGAATCCATGGTGGACGCAGCCGAGACCCTCT
GCCCCAACGTCATGAGAAAAGCCCACATCCGACAGGACCTGATCCATGCCAGCACCGAAAAGATTTCCAT
CCCACGTACCTTTGTTAAAAATGTCCTGTTGGAACAGTCCGGGATTGACATTCTTAACAAAATCAGTGAG
GTGAAGCTGACAGTGGCCTCGTTCTTGTCTGACCGGATCGTCGATGAGATCCTGGATTCTCTCTCCAGCA
GCCATCGGAAGCTGGCCAACCATTTCAGCAGGCTAAACAAGAGCCTTCCCCAGCGAGAGGACCTAGAGGT
GGAGCTGGTGGAGGAGAAGCCGGTGAAACGGGCCATCCTCACGGTAGAGGATCTCACAGAGGTGGAGAGG
CTGGAAGACCTAGACACTTGTATGATGACTCCTAAGTCCAAGAGGAAGAGCATCCACAGCCGAATGCTGC
GGCCTGTCTCCAGGGCCTTCGAAATGGAGTTTGATCTTGATAAAGCCCTGGAAGAGGTGCCGATTCACAT
CGAAGACCCACCTTTCCCATCAGTCCGGCAAGAGAAGCGAAGCTCGGGGTTAATCTCTGAGCTGCCCTCG
GAGGAAGGCAGGAGACTGGAACACTTCACCAAGTTAAGGCCCAAGCGGAACAAGAAGCAGCAACCTACGC
AAGCAGCGGTCTGTACCATCAGCATCTTGCCCCAGGATGGAGAACAGAATGGCCTCATGGGCAGAGTGGA
TGAAGGGGTAGATGAGTTTTTCACCAAGAAGGTGACTAAAATGGATTGCAAGAGGTCATCCTCGCGCTCC
TCAGATGCCCACGAACTCGGGGAAGGAGATGAGAAGAAAAAACGGGACTCTCGGAGGAGTGGCTTCCTCA
ACCTGATTAAATCCAGGTCCAGATCTGAGCGGCCACCCACAGTCCTGATGACGGAAGAGCTCTCCTCCCC
GAAAGGGGCAATGCGGAGCCCACCAGTGGATACCACCAGGAAGGAGATAAAGGCAGCGGAGCACAATGGT
GCTCCAGACCGCACAGAAGAGATTAAGACACCGGAACCCCTGGAGGAGGGTCCAGCAGAGGAAGCTGGCA
GGGCTGAGCGCAGTGACAGCAGGGGCAGCCCGCAGGGTGGCCGGCGCTATGTGCAGGTGATGGGCAGCGG
CCTGCTGGCCGAGATGAAGGCCAAGCAGGAGCGGAGGGCAGCATGTGCACAGAAGAAGCTCGGCAATGAT
GTCATCTCCCAGGATCCTTCCAGCCCAGTCTCGTGCAACACAGAGCGTTTAGAAGGAGGGGCGACAGTGC
CTAAACTGCAACCAGGTCTTCCAGAGGCCCGCTTTGGTTCGGGAACACCAGAAAAGAATGCCAAAGCTGA
ACCCAGAGTGGATGGAGGCTGCAGGTCCCGGAGCTCCTCCAGCATGCCCACCAGCCCAAAGCCCCTCCTT
CAGTCCCCTAAGCCCAGCCCTTCAGCCCGGCCTTCTATCCCTCAGAAGCCAAGAACTGCCTCCCGACCTG
AAGACACCCCAGACTCTCCATCTGGTCCTAGTTCCCCTAAAGTTGCCCTCCTTCCACCCATCCTCAAAAA
AGTCTCCTCGGACAAGGAGCGTGACGGCCAGAACAGCTCACAGTCCAGTCCTAGGAGCTTCTCCCAGGAA
GCCTCAAGGAGAAGCTGGGGCCCGGCCCAGGAGTATCAAGAACAGAAGCAACGGTCCTCGGGCAAAGATG
GTCACCAAGGGAGCAAATGCAGTGACTCTGGAGAAGAGGCAGAAAAAGAGTTTATTTTTGTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215923 representing NM_026825
Red=Cloning site Green=Tags(s)

MTDESSDVPRELMESIKDVIGRKIKISVKKKVKLEVKGDRVENKVLVLTSCRAFLLSARIPSKLELTFSY
LEIHGVICHKPAQMVVETEKCNMSMKMVSPEDVSEVLAHIGTCLRRIFPGLSPLRIMKKVSMEPSERLAS
LQALWDSQTLAEPGPCGGFSQMYACVCDWLGFSYKEEVQWDVDTIYLTQDTRELNLQDFSHLEHRDLIPI
IAALEYNQWFTKLSSKDLKLSTDVCEQILRVVSRSNRLEELVLENAGLRIDFAQKLAGALAHNPNSGLHT
INLAGNSLEDRGVSSLSIQFAKLPKGLKHLNLSKTSLSPKGVNSLCQSLSANPLTASTLTHLDLSGNALR
GDDLSHMYNFLAQPNTIVHLDLSNTECSLEMVCSALLRGCLQCLAVLNLSRSVFSHRKGKEVPPSFKQFF
SSSLALIQINLSGTKLSPEPLKALLLGLACNHSLKGVSLDLSNCELGHCLRSGGAQVLEGCIAEIHNITS
LDISDNGLESDLSTLIVWLSKNRSIQHLALGKNFNNMKSKNLTPVLDNLVQMIQDEDSPLQSLSLADSKL
KAEVTIIINALGSNTSLTKVDISGNGMGDMGAKMLAKALQINTKLRTVIWDKNNITAQGFQDIAVAMEKN
YTLRFMPIPMYDAAQALKTNPEKTEEALQKIENYLLRNHETRKYLQEQAYRLQQGIVTSTTQQMIDRICV
KVQDHLNSLRACGGDAIQEDLKAAERLMRDAKNSKTLLPNLYHVGGASWAGASGLSSSPIQETLESMAGE
VTRVVDEQLKDLLESMVDAAETLCPNVMRKAHIRQDLIHASTEKISIPRTFVKNVLLEQSGIDILNKISE
VKLTVASFLSDRIVDEILDSLSSSHRKLANHFSRLNKSLPQREDLEVELVEEKPVKRAILTVEDLTEVER
LEDLDTCMMTPKSKRKSIHSRMLRPVSRAFEMEFDLDKALEEVPIHIEDPPFPSVRQEKRSSGLISELPS
EEGRRLEHFTKLRPKRNKKQQPTQAAVCTISILPQDGEQNGLMGRVDEGVDEFFTKKVTKMDCKRSSSRS
SDAHELGEGDEKKKRDSRRSGFLNLIKSRSRSERPPTVLMTEELSSPKGAMRSPPVDTTRKEIKAAEHNG
APDRTEEIKTPEPLEEGPAEEAGRAERSDSRGSPQGGRRYVQVMGSGLLAEMKAKQERRAACAQKKLGND
VISQDPSSPVSCNTERLEGGATVPKLQPGLPEARFGSGTPEKNAKAEPRVDGGCRSRSSSSMPTSPKPLL
QSPKPSPSARPSIPQKPRTASRPEDTPDSPSGPSSPKVALLPPILKKVSSDKERDGQNSSQSSPRSFSQE
ASRRSWGPAQEYQEQKQRSSGKDGHQGSKCSDSGEEAEKEFIFV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026825
ORF Size 4122 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026825.3, NP_081101.3
RefSeq Size 5158 bp
RefSeq ORF 4125 bp
Locus ID 68732
UniProt ID Q6EDY6
Cytogenetics 13 A3.1
Summary Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization, however, seems unable to nucleate filaments (PubMed:16054028). Plays a role in lamellipodial protrusion formations and cell migration (PubMed:16054028).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026825" in other vectors (4)
SKU Description Size Price
MC224360 Lrrc16a (untagged) - Mouse leucine rich repeat containing 16A (Lrrc16a), (10ug) 10 ug
$1,294.00
MR215923 Lrrc16a (Myc-DDK-tagged) - Mouse leucine rich repeat containing 16A (Lrrc16a) 10 ug
$589.00 $1,175.00
MR215923L3 Lenti ORF clone of Lrrc16a (Myc-DDK-tagged) - Mouse leucine rich repeat containing 16A (Lrrc16a) 10 ug
$1,475.00
MR215923L4 Lenti ORF clone of Lrrc16a (mGFP-tagged) - Mouse leucine rich repeat containing 16A (Lrrc16a) 10 ug
$1,475.00

file-alt Citations

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