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Bnip1 (NM_172149) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215918
Bnip1 (tGFP-tagged) - Mouse BCL2/adenovirus E1B interacting protein 1 (Bnip1), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Bnip1 or NM_172149"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bnip1
Synonyms 2010005M06Rik; 5930429G21Rik; Sec20
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215918 representing NM_172149
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTCCCCAGGATGTCCACGTCCGGATCTGTAACCAAGAGATTGTCAAATTCGACCTAGAGGTGA
AGGCGCTTATCCAGGATATTCGAGATTGTTCTGGACCCTTAAGTGAGCTTACTGAACTGAATACTAAGGT
GAAAGAAAAGTTCCAACAGTTAAAACAGAGGATCCAGGAGCTCGAACAGTCAGCAAGGGAGCAAGACAAG
GAGTCAGAGAAGCAGCTTCTACTTCAGGAAGTGGAGAACCACAAAAAGCAGATGCTCAGCAACCAGACCT
CATGGCGAAAAGCCAATCTTACCTGCAAGCTTGCCATCGACAACCTGGAGAAGGCAGAACTTCTGCAAGG
AGGAGACTCCTTACGGCAAAGGAAAACCACCAAAGAGAGTTTGGCCCAGACCTCCAGCTCCATCACAGAG
AGTCTCATGGGGATCAGCAGGATGATGTCACAGCAGGTGCAACAGAGCGAGGAGGCTATGCAGACTCTAG
TCAGCTCCTCACGGACTCTCCTGGATGCAAACGAAGAGTTTAAGTCCATGTCGGGAACCATCCAGTTGGG
CCGGAAGCTCATCACAAAGTACAACCGCCGAGAACTGACGGACAAGCTGCTCATCTTCCTGGCCCTGGCC
CTCTTCCTCGCTACAGTCCTTTATATTGTGAAAAAGCGCCTCTTTCCATTTTTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215918 representing NM_172149
Red=Cloning site Green=Tags(s)

MAAPQDVHVRICNQEIVKFDLEVKALIQDIRDCSGPLSELTELNTKVKEKFQQLKQRIQELEQSAREQDK
ESEKQLLLQEVENHKKQMLSNQTSWRKANLTCKLAIDNLEKAELLQGGDSLRQRKTTKESLAQTSSSITE
SLMGISRMMSQQVQQSEEAMQTLVSSSRTLLDANEEFKSMSGTIQLGRKLITKYNRRELTDKLLIFLALA
LFLATVLYIVKKRLFPFL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172149
ORF Size 684 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172149.5, NP_742161.4
RefSeq Size 1100 bp
RefSeq ORF 687 bp
Locus ID 224630
UniProt ID Q6QD59
Cytogenetics 17 A3.3
Summary As part of a SNARE complex may be involved in endoplasmic reticulum membranes fusion and be required for the maintenance of endoplasmic reticulum organization. Plays also a role in apoptosis. It is for instance required for endoplasmic reticulum stress-induced apoptosis. As a substrate of RNF185 interacting with SQSTM1, might also be involved in mitochondrial autophagy.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172149" in other vectors (4)
SKU Description Size Price
MC212729 Bnip1 (untagged) - Mouse BCL2/adenovirus E1B interacting protein 1 (Bnip1), (10ug) 10 ug
$330.00
MR215918 Bnip1 (Myc-DDK-tagged) - Mouse BCL2/adenovirus E1B interacting protein 1 (Bnip1) 10 ug
$289.00 $300.00
MR215918L3 Lenti ORF clone of Bnip1 (Myc-DDK-tagged) - Mouse BCL2/adenovirus E1B interacting protein 1 (Bnip1) 10 ug
$600.00
MR215918L4 Lenti ORF clone of Bnip1 (mGFP-tagged) - Mouse BCL2/adenovirus E1B interacting protein 1 (Bnip1) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.