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Scg3 (NM_009130) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215897
Scg3 (tGFP-tagged) - Mouse secretogranin III (Scg3) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $703.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Scg3
Synonyms 1B1075; AI385542; Chgd; SgIII
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215897 representing NM_009130
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGTTCCTTTGGACCGGCTCTTGGATACTGGTGTTGGTGCTCAACAGCGGCCCAATTCAAGCTTTCC
CCAAACCCGAAGGCAGCCAAGACAAATCCCTGCATAATAGAGAATTAAGTGCAGAAAGACCTTTGAATGA
ACAGATCGCTGAGGCAGAGGCAGACAAGATTAAAAAGGCATTCCCTTCAGAAAGCAAGCCGAGTGAAAGC
AATTATTCTTCTGTCGATAACTTGAATCTGCTGAGGGCAATAACAGAAAAGGAAACCGTTGAGAAAGAGA
GACAATCCATAAGAAGCCCCCCGTTTGATAACCAACTGAACGTGGAAGACGCTGATTCAACCAAAAATCG
GAAACTGATCGATGAGTACGATTCCACCAAGAGTGGACTGGACCACAAGTTTCAAGATGACCCAGACGGC
CTTCATCAACTGGATGGAACTCCTTTAACTGCTGAAGACATCGTCCATAAGATTGCCACCAGGATTTATG
AGGAGAACGACAGAGGAGTGTTTGACAAAATTGTTTCTAAACTGCTGAATCTTGGCCTGATCACTGAAAG
CCAGGCACATACTCTGGAAGATGAAGTAGCAGAAGCTTTACAAAAACTGATTTCAAAAGAGGCCAACAAT
TATGAGGAGACCCTGGATAAACCCACAAGCAGGACCGAGAATCAGGATGGGAAAATACCAGAGAAAGTGA
CTCCGGTGGCAGCAGTCCAAGATGGCTTCACTAACCGTGAAAACGATGAGACGGTGTCTAACACCTTGAC
CTTGTCCAATGGCTTGGAAAGGAGAACTAACCCCCACAGGGAAGACGACTTTGAGGAACTCCAGTATTTC
CCCAACTTCTATGCACTACTGACAAGCATCGACTCAGAAAAAGAAGCAAAAGAGAAAGAAACCCTGATCA
CCATCATGAAGACATTGATTGACTTCGTGAAAATGATGGTGAAATACGGTACGATATCTCCAGAGGAAGG
CGTGTCCTACCTTGAAAACTTGGATGAAACAATTGCTCTGCAGACCAAGAACAAGCTAGAAAAAAATACT
ACTGATAGCAAAAGTAAGCTATTCCCAGCTCCACCAGAGAAGAGTCAGGAAGAAACAGACAGTACCAAGG
AAGAAGCCGCCAAGATGGAAAAGGAATACGGAAGCCTAAAAGACTCTACAAAAGATGATAACTCCAACCT
AGGAGGAAAGACAGATGAAGCCACAGGGAAGACAGAAGCCTACTTGGAAGCCATTAGAAAAAACATCGAA
TGGCTGAAGAAACATAACAAGAAGGGCAACAAAGAAGATTACGACCTTTCAAAGATGAGGGACTTTATCA
ACCAACAAGCTGACGCTTATGTGGAGAAGGGCATCCTCGACAAGGAAGAAGCCAACGCCATCAAACGCAT
CTACAGCAGCCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215897 representing NM_009130
Red=Cloning site Green=Tags(s)

MGFLWTGSWILVLVLNSGPIQAFPKPEGSQDKSLHNRELSAERPLNEQIAEAEADKIKKAFPSESKPSES
NYSSVDNLNLLRAITEKETVEKERQSIRSPPFDNQLNVEDADSTKNRKLIDEYDSTKSGLDHKFQDDPDG
LHQLDGTPLTAEDIVHKIATRIYEENDRGVFDKIVSKLLNLGLITESQAHTLEDEVAEALQKLISKEANN
YEETLDKPTSRTENQDGKIPEKVTPVAAVQDGFTNRENDETVSNTLTLSNGLERRTNPHREDDFEELQYF
PNFYALLTSIDSEKEAKEKETLITIMKTLIDFVKMMVKYGTISPEEGVSYLENLDETIALQTKNKLEKNT
TDSKSKLFPAPPEKSQEETDSTKEEAAKMEKEYGSLKDSTKDDNSNLGGKTDEATGKTEAYLEAIRKNIE
WLKKHNKKGNKEDYDLSKMRDFINQQADAYVEKGILDKEEANAIKRIYSSL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009130
ORF Size 1413 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009130.3, NP_033156.1
RefSeq Size 2207 bp
RefSeq ORF 1416 bp
Locus ID 20255
UniProt ID P47867
Cytogenetics 9 42.32 cM
Summary Member of the granin protein family that regulates the biogenesis of secretory granules (PubMed:16219686). Acts as a sorting receptor for intragranular proteins including chromogranin A/CHGA (PubMed:12388744). May also play a role in angiogenesis (PubMed:28330905). Promotes endothelial proliferation, migration and tube formation through MEK/ERK signaling pathway (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009130" in other vectors (4)
SKU Description Size Price
MC216479 Scg3 (untagged) - Mouse secretogranin III (Scg3), transcript variant 1, (10ug) 10 ug
$503.00
MR215897 Scg3 (Myc-DDK-tagged) - Mouse secretogranin III (Scg3), transcript variant 1 10 ug
$289.00 $503.00
MR215897L3 Lenti ORF clone of Scg3 (Myc-DDK-tagged) - Mouse secretogranin III (Scg3), transcript variant 1 10 ug
$803.00
MR215897L4 Lenti ORF clone of Scg3 (mGFP-tagged) - Mouse secretogranin III (Scg3), transcript variant 1 10 ug
$803.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.