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Efcab10 (NM_029152) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215891
Efcab10 (tGFP-tagged) - Mouse EF-hand calcium binding domain 10 (Efcab10), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Efcab10
Synonyms 4930504H06Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215891 representing NM_029152
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCTGTGGCTGACCGGGAGCTTCAGGCCAAGCTGTATTTAGAAAGACATCGGATCATGGAATTGC
TGAATCAACTTACTAGCTTCCTCCTCTTTGCCCGACCAAAAAAACCAAGAGAGTATTTAATCTCTCTTTT
GGAACGTCTGAGAGTTGCCAAGGCAACACACGTGGCATTTCCCTACTTTATGGACAACTCTAACACTGTG
TCCATGTTTGAGATGATGGACATGGCCGGCAGAGGCTGCATATCATTTGTGCAGTATAAAGAAGCCCTAA
AAAACTTGGGTCTGTGTACTGCAGATGAAGTTTTGAATGATGATGGACATATAATCACTCTGGATACATT
CAGGGATGAAATGAACAAGAGGATGGAAAAAATGTGGTCAATGTTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215891 representing NM_029152
Red=Cloning site Green=Tags(s)

MEPVADRELQAKLYLERHRIMELLNQLTSFLLFARPKKPREYLISLLERLRVAKATHVAFPYFMDNSNTV
SMFEMMDMAGRGCISFVQYKEALKNLGLCTADEVLNDDGHIITLDTFRDEMNKRMEKMWSMF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029152
ORF Size 396 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029152.1, NP_083428.1
RefSeq Size 693 bp
RefSeq ORF 399 bp
Locus ID 75040
UniProt ID Q9D581
Cytogenetics 12 A3
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Documents
Resources
"NM_029152" in other vectors (4)
SKU Description Size Price
MC211583 Efcab10 (untagged) - Mouse EF-hand calcium binding domain 10 (Efcab10), (10ug) 10 ug
$165.00
MR215891 Efcab10 (Myc-DDK-tagged) - Mouse EF-hand calcium binding domain 10 (Efcab10) 10 ug
$289.00
MR215891L3 Lenti ORF clone of Efcab10 (Myc-DDK-tagged) - Mouse EF-hand calcium binding domain 10 (Efcab10) 10 ug
$450.00
MR215891L4 Lenti ORF clone of Efcab10 (mGFP-tagged) - Mouse EF-hand calcium binding domain 10 (Efcab10) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.