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Ap1s3 (NM_183027) Mouse Tagged ORF Clone

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SKU
MG215887
Ap1s3 (tGFP-tagged) - Mouse adaptor-related protein complex AP-1 sigma 3 (Ap1s3), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Ap1s3 or NM_183027"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ap1s3
Synonyms 1190009B22; Jr2; s3A
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215887 representing NM_183027
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATACATTTCATCTTGCTCTTCAGTCGACAAGGGAAGCTGCGGCTGCAGAAATGGTACACCACACTCC
CTGACAAGGAGAGGAAGAAGATCACCCGCGACATCATCCAGACCGTCCTCTCTCGTGGGCACAGGACCAG
CAGCTTCATTGACTGGAAGGAGCTGAAACTTGTTTATAAAAGGTATGCTAGTTTATATTTTTGCTGTGCA
ATAGAAAATCAAGACAATGAGCTCTTGACACTAGAGATTGTTCATCGTTATGTGGAGTTGCTGGATAAAT
ACTTTGGAAATGTCTGTGAGCTGGATATTATCTTTAATTTTGAAAAGGCATATTTTATCCTTGATGAGTT
TATAATAGGTGGAGAAATTCAGGAAACATCCAAGAAAACAGCAGTCAAGGCCATTGAAGACTCTGATATG
TTACAAGAGACAATGGAAGAATACATGAACAAGCCCACGTTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215887 representing NM_183027
Red=Cloning site Green=Tags(s)

MIHFILLFSRQGKLRLQKWYTTLPDKERKKITRDIIQTVLSRGHRTSSFIDWKELKLVYKRYASLYFCCA
IENQDNELLTLEIVHRYVELLDKYFGNVCELDIIFNFEKAYFILDEFIIGGEIQETSKKTAVKAIEDSDM
LQETMEEYMNKPTF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183027
ORF Size 462 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183027.2, NP_898848.1
RefSeq Size 2892 bp
RefSeq ORF 465 bp
Locus ID 252903
UniProt ID Q7TN05
Cytogenetics 1 C4
Summary Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules (By similarity). Involved in TLR3 trafficking (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_183027" in other vectors (4)
SKU Description Size Price
MC213261 Ap1s3 (untagged) - Mouse adaptor-related protein complex AP-1, sigma 3 (Ap1s3), (10ug) 10 ug
$165.00
MR215887 Ap1s3 (Myc-DDK-tagged) - Mouse adaptor-related protein complex AP-1, sigma 3 (Ap1s3) 10 ug
$289.00
MR215887L3 Lenti ORF clone of Ap1s3 (Myc-DDK-tagged) - Mouse adaptor-related protein complex AP-1, sigma 3 (Ap1s3) 10 ug
$450.00
MR215887L4 Lenti ORF clone of Ap1s3 (mGFP-tagged) - Mouse adaptor-related protein complex AP-1, sigma 3 (Ap1s3) 10 ug
$450.00

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