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Lonp1 (NM_028782) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215882
Lonp1 (tGFP-tagged) - Mouse lon peptidase 1 mitochondrial (Lonp1) nuclear gene encoding mitochondrial protein, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,069.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lonp1
Synonyms 1200017E13Rik; LON; Prss15
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215882 representing NM_028782
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGAGCACAGGCTACGTGCGGCTCTGGGCCGCTGCGCGGTGCTGGGTTCTGCGGCGGCCGCTGC
TGGCTGTCACCGGAGGCCGGGTCCCGAGCGCGTCCGGGTCGTGGCTGCGCCGAGGATGTCGGGCCTGCGA
CATGTCAGCTCCCTGGGGCGGCCGCGTTCTTCCCGGCGGGGTCCAGTGGCGGGGGCTGTGGGACTCGGGC
AACCGCGGTGGCAGCGACGAGACCTCCGAGGGCGGTGCGGAGGACGGGGCCACGGCGAGCACCGGGGAAG
GCCCGGTCGTCACAGCGCTCGCGCCCATGACCGTCCCGGATGTGTTTCCTCACCTGCCGCTCATCGCCAT
CACCCGCAACCCTGTGTTCCCGCGCTTTATCAAGATCGTGGAGGTTAAAAATAAGAAGTTGGTTGAGCTC
CTGAGAAGGAAAGTCCGACTTGCACAGCCCTATGTTGGCGTCTTCCTAAAGAGAGATGACAACAATGAGT
CGGACGTGGTGGAGAGCCTGGATGAAATCTACCATACGGGAACATTCGCTCAGATTCATGAGATGCAGGA
CCTCGGAGACAAGTTGCGAATGATTGTCACTGGCCACAGAAGGATTCACATCAGCCGGCAGCTGGAGGTG
GAACCAGAGGGGCTTGAGCCAGAGGCAGAGAAACAAAAGTCCAGGAGGAAGCTGAAGCGAGGCAAGAAAG
AGGTGGAGGATGAGCTGGGCCCCAAGCCCCAGCTGGAGATGGTGACCGAGGCTGCCACTGACACATCCAA
GGAGGTGCTCATGGTGGAGGTTGAGAATGTAGCCCATGAAGACTTCCAGGTCACAGAGGAAGTGAAGGCC
CTGACTGCAGAGATTGTGAAGACCATTCGGGATATCATCGCCTTGAACCCTCTGTACAGAGAGTCCGTGC
TGCAGATGATGCAGGCAGGCCAGCGTGTGGTGGACAACCCCATCTACTTGAGCGACATGGGTGCGGCACT
CACTGGGGCGGAGTCCCATGAGCTGCAAGATGTTCTGGAAGAGACCAACATCCTTAAGCGGCTCTATAAG
GCCCTGTCTCTCTTGAAGAAGGAGTTTGAGTTGAGCAAGCTTCAGCAACGCCTAGGCCGTGAGGTAGAGG
AGAAGATCAAGCAGACACACAGGAAGTACCTGCTGCAGGAGCAGTTGAAGATCATCAAGAAGGAATTGGG
GCTGGAGAAAGATGATAAAGATGCCATTGAGGAGAAGTTCCGGGAACGCCTCAGGGAGCTTGTGGTTCCC
AAGCATGTGATGGACGTGGTGGATGAGGAGCTGAGCAAGCTTGCTCTACTGGACAACCACTCCTCTGAGT
TCAATGTCACTCGAAACTACCTGGACTGGCTGACATCCATCCCATGGGGCCGGCAGAGTGATGAGAACTT
GGACTTGGCTCGGGCCCAGGCTGTGCTGGAGGAGGACCACTACGGCATGGAGGATGTGAAGAAGCGTGTC
CTGGAGTTCATTGCAGTCAGCCAGCTCAGAGGCTCCACACAAGGCAAGATCCTCTGCTTCCACGGCCCAC
CGGGGGTGGGCAAGACCAGCATTGCACGCTCCATTGCCCGTGCCCTTGGCCGAGAATACTTCCGTTTCAG
TGTTGGTGGCATGACAGATGTGGCAGAAATCAAGGGGCACAGGCGTACCTACGTGGGGGCCATGCCTGGG
AAGATCATCCAGTGTCTGAAGAAGACCAAGACAGAGAACCCGCTAGTGCTCATTGATGAGGTGGACAAGA
TTGGCCGAGGCTACCAAGGGGACCCGTCATCAGCACTGCTGGAGTTGCTGGACCCTGAGCAGAATGCCAA
CTTCCTGGACCACTACCTGGATGTGCCAGTGGATCTGTCCAAGGTGCTATTCATCTGCACGGCCAATGTC
ATTGACACCATCCCAGAACCACTGAGGGACCGCATGGAGATGATCAATGTGTCTGGCTATGTAGCACAGG
AGAAGCTGGCCATCGCAGAGCGGTACCTGGTGCCACAGGCCCGCACCCTGTGTGGCCTAGATGAGAGCAA
GGCCCAGCTGTCAGCAGCAGTGCTCACACTGCTCATCAAGCAATACTGCAGGGAGAGTGGTGTGCGCAAC
CTACAGAAGCAGGTGGAGAAGGTGCTGCGCAAGGCTGCATACAAGATCGTAAGTGGGGAGGCCCAGACAG
TACAGGTGACACCTGAGAACCTACAAGACTTTGTTGGGAAGCCTGTGTTCACAGTGGAGCGCATGTACGA
AGTCACACCTCCTGGCGTGGTCATGGGTTTGGCCTGGACTGCCATGGGGGGCTCTACATTGTTTGTGGAG
ACATCTTTGAGGCGGCCCCAGCCTAGTGGCAGCAAGGAGGACAAGGATGGCAGCCTAGAGGTGACAGGTC
AGCTGGGAGATGTGATGAAAGAGAGTGCCCGCATAGCCTACACCTATGCTCGTGCCTTCCTGATGGAGCA
GGACCCTGAAAATGACTTCCTAGTCACCTCACACATCCACCTGCATGTGCCTGAGGGTGCTACCCCCAAG
GATGGTCCCAGCGCAGGCTGTACTATTGTCACTGCACTGCTGTCCCTGGCTCTGGGCCAGCCAGTGTTGC
AGAACCTGGCTATGACTGGGGAGGTCTCCCTCACTGGCAAGGTGTTGCCTGTGGGTGGCATCAAGGAGAA
GACCATTGCGGCTAAGCGTGCAGGTGTGACCTGCATCATTCTGCCTGCTGAGAACAGAAAGGACTACTCG
GATTTAGCTCCTTTTATCACAGAGGGCCTTGAAGTTCACTTCGTGGAACACTATCGGGACATATTCCCCA
TTGCCTTCCCCCGGCGAGAGCACAGGGAGGCACTGGCCGTGGAGAGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215882 representing NM_028782
Red=Cloning site Green=Tags(s)

MAASTGYVRLWAAARCWVLRRPLLAVTGGRVPSASGSWLRRGCRACDMSAPWGGRVLPGGVQWRGLWDSG
NRGGSDETSEGGAEDGATASTGEGPVVTALAPMTVPDVFPHLPLIAITRNPVFPRFIKIVEVKNKKLVEL
LRRKVRLAQPYVGVFLKRDDNNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVTGHRRIHISRQLEV
EPEGLEPEAEKQKSRRKLKRGKKEVEDELGPKPQLEMVTEAATDTSKEVLMVEVENVAHEDFQVTEEVKA
LTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNILKRLYK
ALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDKDAIEEKFRERLRELVVP
KHVMDVVDEELSKLALLDNHSSEFNVTRNYLDWLTSIPWGRQSDENLDLARAQAVLEEDHYGMEDVKKRV
LEFIAVSQLRGSTQGKILCFHGPPGVGKTSIARSIARALGREYFRFSVGGMTDVAEIKGHRRTYVGAMPG
KIIQCLKKTKTENPLVLIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSKVLFICTANV
IDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARTLCGLDESKAQLSAAVLTLLIKQYCRESGVRN
LQKQVEKVLRKAAYKIVSGEAQTVQVTPENLQDFVGKPVFTVERMYEVTPPGVVMGLAWTAMGGSTLFVE
TSLRRPQPSGSKEDKDGSLEVTGQLGDVMKESARIAYTYARAFLMEQDPENDFLVTSHIHLHVPEGATPK
DGPSAGCTIVTALLSLALGQPVLQNLAMTGEVSLTGKVLPVGGIKEKTIAAKRAGVTCIILPAENRKDYS
DLAPFITEGLEVHFVEHYRDIFPIAFPRREHREALAVER

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028782
ORF Size 2847 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028782.2, NP_083058.2
RefSeq Size 2970 bp
RefSeq ORF 2850 bp
Locus ID 74142
UniProt ID Q8CGK3
Cytogenetics 17 D
Summary ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner. May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028782" in other vectors (4)
SKU Description Size Price
MC222791 Lonp1 (untagged) - Mouse lon peptidase 1, mitochondrial (Lonp1), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$957.00
MR215882 Lonp1 (Myc-DDK-tagged) - Mouse lon peptidase 1, mitochondrial (Lonp1), nuclear gene encoding mitochondrial protein 10 ug
$589.00 $869.00
MR215882L3 Lenti ORF clone of Lonp1 (Myc-DDK-tagged) - Mouse lon peptidase 1, mitochondrial (Lonp1), nuclear gene encoding mitochondrial protein 10 ug
$1,169.00
MR215882L4 Lenti ORF clone of Lonp1 (mGFP-tagged) - Mouse lon peptidase 1, mitochondrial (Lonp1), nuclear gene encoding mitochondrial protein 10 ug
$1,169.00

Plasmid Map for MG215882

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG215882
Circular map for MG215882

file-alt Citations

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