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Dcaf6 (NM_028759) Mouse Tagged ORF Clone

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SKU
MG215880
Dcaf6 (tGFP-tagged) - Mouse DDB1 and CUL4 associated factor 6 (Dcaf6), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,002.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dcaf6
Synonyms 1200006M05Rik; Iqwd1; PC326
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215880 representing NM_028759
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCGGAGTGGCTCCTGCCCGCACCTGTTGTGGGACGTGAGGAAAAGGTCCCTTGGGCTGGAGGACC
CGTCCCGGCTGAGGAGCCGCTACCTGGGAAGAAGAGAATTTATCCAAAGATTAAAACTTGAAGCAACTTT
AAATGTGCATGATGGCTGTGTTAATACAATCTGTTGGAATGACACTGGAGAATATATTTTATCTGGCTCT
GATGACACTAAACTTGTAATTAGTAATCCATACAGCAGAAAGGTTTTGACAACCATCCGTTCAGGGCATC
GAGCAAATATATTTAGTGCAAAGTTTTTGCCGTGCACAGATGATAAGCAGATTGTGTCTTGCTCTGGAGA
TGGAGTCATATTTTATACTAACATTGAGCAAGATGCAGAAACTAACAGACAGTGCCAATTTACGTGCCAT
TATGGAACTACTTATGAGATTATGACTGTACCAAACGACCCTTACACCTTTCTGTCCTGTGGTGAAGATG
GAACTGTTAGGTGGTTTGACACACGCATCAAAACCAGTTGCACAAAAGAAGACTGTAAAGATGATATTTT
AATCAACTGTAGGCGTGCTGCCACATCTGTGGCTATTTGTCCCCCAGTACCATATTACCTTGCTGTGGGT
TGTTCTGACAGCTCAGTACGGATTTATGATCGGCGAATGCTGGGCACAAGAGCTACAGGGAATTATGCAG
GCCGAGGAACTACTGGAATGGTTGCTCGATTTATACCTTCTCATCTTAGTAACAAATCATGCAGAGTGAC
ATCACTGTGTTACAGTGAAGATGGTCAAGAGATTCTTGTCAGTTATTCTTCAGACTACATCTATCTTTTT
GACCCCAAAGATGATACTGCACGAGAACTTAAAACTCCTTCTGCAGAGGAGAGGCGAGAAGAGTTACGAC
AGCCTCCAGTTAAGCGCTTGAGACTTCGTGGTGATTGGTCAGATACTGGTCCCAGAGCACGGCCAGAAAG
TGAACGAGAACGAGATGGAGAGCAAAGTCCCAATGTGTCACTGATGCAGAGAATGTCTGATATGTTATCA
AGGTGGTTTGAAGAAGCAAGTGAAGTTGCACAAAGCAACAGAGGAAGAGGAAGACCTCGGCCCAGAGGTG
GAACAAATCAGCCAGATGTTTCAACTCTTCCTACGGTTCCATCAAGTCCTAATTTGGAAGTGTGTGAAAC
TGCAATGGATGTAGACATGCCAGCTGCACTTCTTCAGCCTTCTACATCCTCTACAGATCCAGTTCAGGCT
CAGGCAGCCACAGCCGCCATAGAAAGCCCTCGTTCCAGCTCGTTGCTGTCTTGCCCAGACAGTGAACCGA
GGCAGTCTGTTGAGGCGTCTGGACACCATGCACATCATCAGTCAGATAACAGTAATGAGAGGCTGAGCCC
CAAACCAGGGACAGGTGAACCCGTTTTAAGTTTGCACTACAGCACAGAAGGAACAACTACAAGCACAATA
AAACTGAACTTTACAGATGAATGGAGCAGTACAGCCTCAAGTTCCAGAGGAAATGGGAGCCATTGCAAAT
CTGAGGGTCAGGAAGAATGCTTGGTCCCTCCGAGCTCTGTGCAGCCACCGGAAGGAGACAGTGAAACAAG
AGCTCCTGAAGAACTATCAGAGAAAGGAACACTTCCAGAAAACCTCACTCAAAACCAGATAGATACAGCA
CAACTTGATAACTTCCCAGCTGAGCCATTGGATTCTAACTCAGGAGAGAAGAATAACCCAAGTCAGGACA
GCCCTTGTGGGCTTCCAGAAGAAGGCACTTTGTCTGAAACAGACAGGGAGACTTGTGAGCAGGCCAGCAC
TGAGAGTGCTACCAGGCATGCTAGCACCAAGCCTGAACTCCCATCCCAGACAGAAGCCATTGAGCAGGCC
AGCACTGAGAGTGCTACCAGGCATACCAGTGCCAATCCTGAACTCCCATCCCAGACAGAAGCCATAGCAC
CTTTAGCTCATGAAGACCCATCTGCCAGGGACTCTGCTCTCCAGGACACAGATGACAGCGATGATGATCC
GGTCTTGATCCCTGGTGCAAGATACCGAACAGGACCTGGTGATAGACGCTCCGCTGTTGCCCGCATTCAG
GAGTTCTTCAGGAGGAGAAAAGAAAGGAAAGAAATGGAAGAGCTGGATACTTTGAACATTAGGAGGCCAC
TAGTAAAGATGGTTTATAAGGGCCACCGCAACTCCCGGACAATGATAAAAGAAGCCAATTTCTGGGGTGC
TAACTTTGTAATGAGCGGTTCCGATTGTGGCCATATCTTCATCTGGGACCGGCACACTGCTGAGCATTTG
ATGCTTCTGGAAGCTGATAATCATGTGGTCAACTGCCTGCAGCCCCATCCGTTTGACCCAATTCTAGCCT
CATCTGGCATAGATTATGACATAAAGATCTGGTCGCCACTAGAAGAGTCAAGAATTTTTAATCGAAAACT
TGCTGATGAAGTTATAACTCGGAATGAACTCATGCTGGAAGAGACTCGGAACACCATCACCGTCCCAGCC
TCTTTCATGTTGAGGATGTTGGCGTCACTGAATCATATCCGAGCTGACCGTCTGGAGGGTGACAGATCAG
AAGGTTCAGGTCAGGAGAATGAAAATGAGGATGAAGAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215880 representing NM_028759
Red=Cloning site Green=Tags(s)

MARSGSCPHLLWDVRKRSLGLEDPSRLRSRYLGRREFIQRLKLEATLNVHDGCVNTICWNDTGEYILSGS
DDTKLVISNPYSRKVLTTIRSGHRANIFSAKFLPCTDDKQIVSCSGDGVIFYTNIEQDAETNRQCQFTCH
YGTTYEIMTVPNDPYTFLSCGEDGTVRWFDTRIKTSCTKEDCKDDILINCRRAATSVAICPPVPYYLAVG
CSDSSVRIYDRRMLGTRATGNYAGRGTTGMVARFIPSHLSNKSCRVTSLCYSEDGQEILVSYSSDYIYLF
DPKDDTARELKTPSAEERREELRQPPVKRLRLRGDWSDTGPRARPESERERDGEQSPNVSLMQRMSDMLS
RWFEEASEVAQSNRGRGRPRPRGGTNQPDVSTLPTVPSSPNLEVCETAMDVDMPAALLQPSTSSTDPVQA
QAATAAIESPRSSSLLSCPDSEPRQSVEASGHHAHHQSDNSNERLSPKPGTGEPVLSLHYSTEGTTTSTI
KLNFTDEWSSTASSSRGNGSHCKSEGQEECLVPPSSVQPPEGDSETRAPEELSEKGTLPENLTQNQIDTA
QLDNFPAEPLDSNSGEKNNPSQDSPCGLPEEGTLSETDRETCEQASTESATRHASTKPELPSQTEAIEQA
STESATRHTSANPELPSQTEAIAPLAHEDPSARDSALQDTDDSDDDPVLIPGARYRTGPGDRRSAVARIQ
EFFRRRKERKEMEELDTLNIRRPLVKMVYKGHRNSRTMIKEANFWGANFVMSGSDCGHIFIWDRHTAEHL
MLLEADNHVVNCLQPHPFDPILASSGIDYDIKIWSPLEESRIFNRKLADEVITRNELMLEETRNTITVPA
SFMLRMLASLNHIRADRLEGDRSEGSGQENENEDEE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028759
ORF Size 2628 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028759.1, NP_083035.1
RefSeq Size 3306 bp
RefSeq ORF 2631 bp
Locus ID 74106
UniProt ID Q9DC22
Cytogenetics 1 H2.2- H2.3
Summary Ligand-dependent coactivator of nuclear receptors. Enhance transcriptional activity of the nuclear receptors NR3C1 and AR. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028759" in other vectors (4)
SKU Description Size Price
MC222333 Dcaf6 (untagged) - Mouse DDB1 and CUL4 associated factor 6 (Dcaf6), (10ug) 10 ug
$883.00
MR215880 Dcaf6 (Myc-DDK-tagged) - Mouse DDB1 and CUL4 associated factor 6 (Dcaf6) 10 ug
$589.00 $802.00
MR215880L3 Lenti ORF clone of Dcaf6 (Myc-DDK-tagged) - Mouse DDB1 and CUL4 associated factor 6 (Dcaf6) 10 ug
$1,102.00
MR215880L4 Lenti ORF clone of Dcaf6 (mGFP-tagged) - Mouse DDB1 and CUL4 associated factor 6 (Dcaf6) 10 ug
$1,102.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.