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Adar (NM_001146296) Mouse Tagged ORF Clone

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SKU
MG215879
Adar (tGFP-tagged) - Mouse adenosine deaminase RNA-specific (Adar) transcript variant 3, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,328.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Adar or NM_001146296"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adar
Synonyms Adar1; Adar1p110; Adar1p150; AV242451; mZaADAR
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215879 representing NM_001146296
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCAAGGGTTCAGGGGACCCACAGGGGTGTTCCCTCACCAGACACAGTCGTACTTGGACCCTAGTC
ATGAGCATAGCAAGTGGAGATACCCGCAGCCACAGGGGCCGGAGTCTTACCCTAGGAGTTTCCAGCTTCA
GCAGATAGAGTTTCTCAAAGGGCGGCTCCCAGAAGCACCCTTGATTGGAATACAAACCCAGTCACTGCCG
CCATTCCTCCCAGGACACTGGCCAAGATTCCCAGGGCCACCTGCCCAAGACAGGCAACTGGAAATCTGGG
AGTTCCCCAGGAGTGTGACTCTCAGAAATCAGGGGTTCCACATAGGACCCCCACTTCCTCCCCCACACAG
CAGGGGTACACCATGGAGAGGTGCTGACGGGCTTTGCTCACACTTCCGGGAGCTGAGCATCAGTCAGAGT
CCGGAGCAGAAGGTTCTAAACCGCCTGGAAGAGCTTGGGGAGGGGAAGGCCACCACTGCCCATGTGCTAG
CCAGAGAGCTCAGAATCCCCAAAAGGGACATCAATCGTATTTTGTACTCCCTGGAAAAGAAGGGAAAGCT
GCACAGAGGAAGGGGGAAACCTCCTTTGTGGAGCCTTGTGCCCTTGAGTCAGGCTTGGACTCAGCCCCCT
GGAGTTGTGAATCCAGATAGTTGTATCCAGGAATTCCCTAGAGGAGAGCCTGGTTTGGACAGTGAGGACG
GAGACCCTGCCTCTGACTTAGAAGGACCTTCTGAGCCTCTTGACATGGCTGAAATCAAGGAGAAGATCTG
TGACTATCTGTTCAATGTGTCAAACTCCTCTGCCCTGAACCTGGCTAAGAACATTGGCCTCACCAAGGCC
CGAGATGTGACCTCAGTGCTGATTGACTTGGAAAGGCAAGGCGATGTCTACAGGCAAGGGGCAACTCCTC
CCATCTGGTACTTGACGGACAAGAAGCGTGAGAGGCTGCAGATGAAGAGAAGTACACACAGTGCTCCTGC
CCCTACCCCGACAGCTGTCCCAGAGGCCACTAGAAGCCCCTCATTCCCTGCCTGCCACCCGCCCCCAGCA
GGTGCCTCAAGCAGTGTGGCAGCCTCCAAGAGAGTGGAGAATGGGCAGGAGCCTGCGATAAAGCATGAAA
GTAGGCATGAGGCCAGACCAGGACCAATGAGACTGCGGCCTCACGCTTATCACAATGGCCCCTCTAGAGC
AGGGTATGTGGCCTCTGAAAATGGCCAGTGGGCCACAGATGACATCCCAGATAACTTGAATAGTATCCAC
ACAGCACCAGGTGAGTTTCGAGCCATCATGGAGATGCCCTCCTTCTACAGCCCTACCTTGCCACGGTGTT
CACCCTACAAGAAGCTAACTGAGTGCCAGCTGAAGAACCCTGTCAGCGGGTTGTTAGAGTATGCTCAGTT
CACTAGTCAGACCTGTGATTTCAACCTGATAGAGCAGAGTGGACCGTCCCATGAACCTCGATTTAAATTC
CAGGTTGTCATCAATGGGCGGGAATTTCCCCCAGCTGAGGCTGGCAGCAAGAAAGTAGCCAAGCAGGACG
CAGCAGTGAAAGCCATGGCGATTCTGCTTCGGGAAGCCAAAGCCAAAGACAGTGGTCAACCAGAAGACTT
GTCCCACTGTCCCATGGAAGAAGACTCGGAGAAACCAGCAGAGGCTCAGGCCCCCAGCTCCTCAGCAACA
TCCTTGTTCTCTGGGAAGAGCCCAGTTACTACACTGCTTGAGTGCATGCACAAACTAGGGAACTCCTGTG
AATTCCGTCTCCTGTCCAAAGAAGGCCCTGCTCATGACCCCAAGTTCCAGTACTGTGTAGCAGTAGGAGC
CCAGACCTTCCCCCCTGTGAGCGCCCCCAGCAAGAAGGTAGCAAAGCAGATGGCCGCAGAGGAAGCCATG
AAGGCGCTGCAGGAGGAGGCAGCCAGTTCAGCGGATGACCAGTCTGGAGGTGCGAACACAGACTCACTTG
ATGAATCTATGGCTCCCAACAAGATCAGGAGGATTGGTGAGCTCGTCAGGTACCTGAACACCAACCCCGT
AGGCGGCTTGTTGGAGTACGCCCGATCTCATGGCTTTGCTGCTGAGTTCAAGCTCATTGACCAGTCTGGA
CCTCCTCACGAACCCAAGTTTGTTTACCAAGCAAAAGTTGGGGGCCGCTGGTTTCCAGCCGTGTGTGCAC
ACAGCAAGAAACAGGGCAAGCAGGATGCAGCGGATGCAGCCCTCCGGGTCTTGATCGGGGAGAGCGAGAA
GGCAGAGCAGTTGGGTTTCGCAGAGGTAACCCCAGTAACAGGGGCCAGTCTCAGAAGAACTATGCTCCTC
CTTTCCAGGTCCCCAGATGCACATCCAAAGACACTTCCTCTCTCTGGCAGCACCTTCCACGACCAGATAG
CTATGCTGAGCCACAGGTGCTTCAATGCTCTGACCAACAGTTTCCAGCCCTCCCTGCTCGGCCGCAAGAT
CCTGGCTGCCATTATTATGAAAAGAGATCCAGAGGACATGGGTGTTGTCGTGAGTTTGGGGACAGGCAAT
CGCTGTGTGAAAGGGGACTCTCTCAGCCTGAAGGGAGAGACGGTCAATGACTGCCATGCCGAAATCATCT
CCCGGAGGGGCTTCATCAGGTTTCTCTACAGTGAACTGATGAAGTACAACCACCACACTGCCAAGAACAG
CATATTTGAGCTTGCCAGGGGAGGAGAGAAGCTGCAGATAAAGAAGACGGTTTCTTTTCATCTCTACATC
AGCACGGCGCCATGTGGAGATGGGGCCCTCTTTGACAAATCCTGCAGTGACCGTGCCGTGGAAAGCACAG
AGTCCCGCCATTACCCTGTCTTTGAAAATCCCAAGCAAGGCAAGCTTCGCACCAAGGTGGAGAATGGGGA
AGGCACAATTCCTGTGGAGTCCAGTGATATTGTACCCACGTGGGATGGCATCCGCCTTGGGGAAAGACTC
CGTACCATGTCCTGTAGTGACAAAATCCTACGCTGGAATGTGCTGGGCCTGCAAGGGGCGTTGTTGACGC
ACTTCCTACAGCCTGTGTACCTGAAATCTGTAACCTTAGGTTACCTTTTCAGCCAAGGGCATCTGACCCG
TGCTATTTGCTGCCGCGTGACCAGAGATGGGAAAGCATTTGAGGATGGACTACGCTATCCCTTTATTGTC
AACCACCCCAAGGTCGGCCGAGTCAGTGTTTATGATTCCAAAAGACAGTCCGGAAAGACCAAGGAAACAA
GCGTCAACTGGTGCATGGCTGATGGCTATGACCTAGAGATCCTGGATGGCACCAGAGGCACTGTGGATGG
ACCAGGGAAAGAGTTGTCTCGGGTGTCCAAGAAGAATATTTTCCTTCAGTTTAAGAAGCTCTGCTCCTTC
CGAGCCCGCAGAGATTTACTGCAGCTCTCTTATGGTGAAGCCAAGAAAGCTGCCCGTGACTATGACTTAG
CCAAGAACTACTTCAAGAAAAGCCTGCGAGACATGGGCTATGGGAATTGGATCAGCAAACCCCAGGAGGA
AAAGAACTTTTACCTCTGTCCAGTACCCAATGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215879 representing NM_001146296
Red=Cloning site Green=Tags(s)

MSQGFRGPTGVFPHQTQSYLDPSHEHSKWRYPQPQGPESYPRSFQLQQIEFLKGRLPEAPLIGIQTQSLP
PFLPGHWPRFPGPPAQDRQLEIWEFPRSVTLRNQGFHIGPPLPPPHSRGTPWRGADGLCSHFRELSISQS
PEQKVLNRLEELGEGKATTAHVLARELRIPKRDINRILYSLEKKGKLHRGRGKPPLWSLVPLSQAWTQPP
GVVNPDSCIQEFPRGEPGLDSEDGDPASDLEGPSEPLDMAEIKEKICDYLFNVSNSSALNLAKNIGLTKA
RDVTSVLIDLERQGDVYRQGATPPIWYLTDKKRERLQMKRSTHSAPAPTPTAVPEATRSPSFPACHPPPA
GASSSVAASKRVENGQEPAIKHESRHEARPGPMRLRPHAYHNGPSRAGYVASENGQWATDDIPDNLNSIH
TAPGEFRAIMEMPSFYSPTLPRCSPYKKLTECQLKNPVSGLLEYAQFTSQTCDFNLIEQSGPSHEPRFKF
QVVINGREFPPAEAGSKKVAKQDAAVKAMAILLREAKAKDSGQPEDLSHCPMEEDSEKPAEAQAPSSSAT
SLFSGKSPVTTLLECMHKLGNSCEFRLLSKEGPAHDPKFQYCVAVGAQTFPPVSAPSKKVAKQMAAEEAM
KALQEEAASSADDQSGGANTDSLDESMAPNKIRRIGELVRYLNTNPVGGLLEYARSHGFAAEFKLIDQSG
PPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQDAADAALRVLIGESEKAEQLGFAEVTPVTGASLRRTMLL
LSRSPDAHPKTLPLSGSTFHDQIAMLSHRCFNALTNSFQPSLLGRKILAAIIMKRDPEDMGVVVSLGTGN
RCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELMKYNHHTAKNSIFELARGGEKLQIKKTVSFHLYI
STAPCGDGALFDKSCSDRAVESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVPTWDGIRLGERL
RTMSCSDKILRWNVLGLQGALLTHFLQPVYLKSVTLGYLFSQGHLTRAICCRVTRDGKAFEDGLRYPFIV
NHPKVGRVSVYDSKRQSGKTKETSVNWCMADGYDLEILDGTRGTVDGPGKELSRVSKKNIFLQFKKLCSF
RARRDLLQLSYGEAKKAARDYDLAKNYFKKSLRDMGYGNWISKPQEEKNFYLCPVPND

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001146296
ORF Size 3534 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001146296.1, NP_001139768.1
RefSeq Size 5928 bp
RefSeq ORF 3537 bp
Locus ID 56417
UniProt ID Q99MU3
Cytogenetics 3 F1
Summary Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Does not affect polyomavirus replication but provides protection against virus-induced cytopathic effects. Essential for embryonic development and cell survival and plays a critical role in the maintenance of hematopoietic stem cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001146296" in other vectors (3)
SKU Description Size Price
MR215879 Adar (Myc-DDK-tagged) - Mouse adenosine deaminase, RNA-specific (Adar), transcript variant 3 10 ug
$1,128.00
MR215879L3 Lenti ORF clone of Adar (Myc-DDK-tagged) - Mouse adenosine deaminase, RNA-specific (Adar), transcript variant 3 10 ug
$1,428.00
MR215879L4 Lenti ORF clone of Adar (mGFP-tagged) - Mouse adenosine deaminase, RNA-specific (Adar), transcript variant 3 10 ug
$1,428.00

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