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Papolb (NM_019943) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215877
Papolb (tGFP-tagged) - Mouse poly (A) polymerase beta (testis specific) (Papolb), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $798.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Papolb
Synonyms PAP; Paplob; Papola-ps; Papt; Plap-ps; Tpap
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215877 representing NM_019943
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCCATTTGCGGTGACCACCCAAGGAGCACAACAACCGGCTCCCGCCCCGAAGCAGTTCGGCATAT
CGTCCCCCATCAGCCTGGCCGCCCCCAAGGACACAGACCGCGAACTCACCCAGAAGCTGATCGAGACCCT
CCAGCCCTTCGGGGTGTTTGAAGAGGAAGAGGAACTGCAGCGCAGGATTTTAATTTTGCAGAAATTAAAT
AATCTGGTGAAGGAATGGATCCGAGAAATCAGTGAAAGCAGGAATCTTCCACAAGCTGTAATTGAGAATG
TTGGGGGGAAAATTTTTACGTTCGGCTCTTACCGATTAGGAGTACACACGAAAGGTGCAGATATCGATGC
TTTGTGCGTTGCACCAAGACACGTGGATCGAAATGACTTTTTCACCTCCTTCTATGACAAATTGAAACTA
CAAGAGGAAGTAAAAGATTTAAGAGCTGTTGAGGAGGCCTTTGTGCCGGTTATCAAACTGTGTTTTGATG
GAATAGAGATTGATATTTTGTTTGCAAGATTAGCATTGCAGACTATTCCAGAAGATTTGGACCTACGAGA
TGATAGTCTGCTTAAAAATTTAGATATTAGGTGCATAAGAAGCCTTAACGGTTGCCGGGTAACTGATGAG
ATTTTACATCTAGTACCAAACATTGACAGCTTCAGGTTAACACTGAGAGCCATCAAATTATGGGCCAAAT
GCCACAACATCTATTCCAATATATTGGGTTTCCTTGGAGGTGTTTCCTGGGCTATGCTAGTAGCAAGAAC
TTGCCAGCTTTACCCAAATGCAATAGCATCCACTCTTGTGCGAAAATTTTTCTTGGTGTTTTCTGAGTGG
GAATGGCCAAATCCAGTGTTGTTAAAAGAACCAGAAGAACGAAATCTTAACTTGCCTGTATGGGACCCAA
GAGTGAATCCTAGTGACAGGTACCATCTTATGCCTATAATTACACCAGCATACCCACAGCAGAACTCCAC
GTACAATGTGTCTGTTTCGACCAGGATGGTCATGATTGAGGAGTTTAAACAAGGACTTGCTATCACACAT
GAAATTCTGCTGAATAAGGCAGAATGGTCCAAACTTTTTGAAGCTCCAAGCTTTTTTCAAAAATACAAGC
ATTATATTGTACTTCTGGCAAGTGCGCCAACAGAGAAACAACATTTAGAGTGGGTGGGCTTGGTGGAATC
AAAGATCCGAATCCTGGTTGGGAGCCTGGAAAAGAATGAGTTCATTACTCTGGCTCATGTGAATCCTCAG
TCGTTTCCAGCACCAAAAGAAACTGCTGACAAGGAAGAATTTCGTACTATGTGGGTGATCGGGTTAGTGT
TGAAGAAGCCAGAAAACTCTGAAATTCTCAGTATTGATCTCACCTATGATATTCAGTCTTTCACAGATAC
AGTGTACAGACAAGCAATAAATAGTAAGATGTTTGAGATGGATATGAAGATTGCTGCAATGCATCTAAGA
AGGAAGGAACTTCATCAGCTACTTCCAAATCATGTTCTTCAGAAAAAGGAAACCCATTTAACAGAAAGTG
TAAGGCTGACAGCTGTGACTGACAGCAGCCTTCTCTTGTCTATAGACAGTGAAAACAGCATGACTGCTCC
TTCACCTACTGGCACTATGAAGACAGGTCCACTGACTGGAAACCCTCAGGGCAGAAACAGTCCTGCTCTG
GCTGTTATGGCAGCATCTGTGACCAACATACAGTTTCCTGATGTTTCCTTGCAGCATGTGAATCCCATAG
AAAGCTCAGGAATTGCACTGAGTGAAAGCATCCCTCAGATTCCCTCACAACCTACCATTTCACCACCACC
TAAGCCTACAATGACCAGAGTGGTTTCTTCGACACATCTGGTAAACCATCCATCTAGACCTTCAGGAAAT
ACAGCAACGAACATACCCAATCCTATACTAGGAGTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215877 representing NM_019943
Red=Cloning site Green=Tags(s)

MMPFAVTTQGAQQPAPAPKQFGISSPISLAAPKDTDRELTQKLIETLQPFGVFEEEEELQRRILILQKLN
NLVKEWIREISESRNLPQAVIENVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVDRNDFFTSFYDKLKL
QEEVKDLRAVEEAFVPVIKLCFDGIEIDILFARLALQTIPEDLDLRDDSLLKNLDIRCIRSLNGCRVTDE
ILHLVPNIDSFRLTLRAIKLWAKCHNIYSNILGFLGGVSWAMLVARTCQLYPNAIASTLVRKFFLVFSEW
EWPNPVLLKEPEERNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSVSTRMVMIEEFKQGLAITH
EILLNKAEWSKLFEAPSFFQKYKHYIVLLASAPTEKQHLEWVGLVESKIRILVGSLEKNEFITLAHVNPQ
SFPAPKETADKEEFRTMWVIGLVLKKPENSEILSIDLTYDIQSFTDTVYRQAINSKMFEMDMKIAAMHLR
RKELHQLLPNHVLQKKETHLTESVRLTAVTDSSLLLSIDSENSMTAPSPTGTMKTGPLTGNPQGRNSPAL
AVMAASVTNIQFPDVSLQHVNPIESSGIALSESIPQIPSQPTISPPPKPTMTRVVSSTHLVNHPSRPSGN
TATNIPNPILGV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019943
ORF Size 1926 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019943.2, NP_064327.1
RefSeq Size 2337 bp
RefSeq ORF 1929 bp
Locus ID 56522
UniProt ID Q9WVP6
Cytogenetics 5 G2
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Documents
Resources
"NM_019943" in other vectors (4)
SKU Description Size Price
MC219993 Papolb (untagged) - Mouse poly (A) polymerase beta (testis specific) (Papolb), (10ug) 10 ug
$658.00
MR215877 Papolb (Myc-DDK-tagged) - Mouse poly (A) polymerase beta (testis specific) (Papolb) 10 ug
$289.00 $598.00
MR215877L3 Lenti ORF clone of Papolb (Myc-DDK-tagged) - Mouse poly (A) polymerase beta (testis specific) (Papolb) 10 ug
$898.00
MR215877L4 Lenti ORF clone of Papolb (mGFP-tagged) - Mouse poly (A) polymerase beta (testis specific) (Papolb) 10 ug
$898.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.