Depdc5 (NM_177786) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG215873
Depdc5 (tGFP-tagged) - Mouse DEP domain containing 5 (Depdc5) transcript variant 2, (10ug)
$1,144.00
3 Weeks*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Depdc5 |
| Synonyms | AV016528 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_177786 |
| ORF Size | 3255 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_177786.4, NP_808454.2 |
| RefSeq Size | 3569 bp |
| RefSeq ORF | 3258 bp |
| Locus ID | 277854 |
| Cytogenetics | 5 17.35 cM |
| Summary | As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_177786" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC223497 | Depdc5 (untagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 2, (10ug) | 10 ug |
$1,040.00
|
|
| MR215873 | Depdc5 (Myc-DDK-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 2 | 10 ug |
$589.00
$944.00
|
|
| MR215873L3 | Lenti ORF clone of Depdc5 (Myc-DDK-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 2 | 10 ug |
$1,244.00
|
|
| MR215873L4 | Lenti ORF clone of Depdc5 (mGFP-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 2 | 10 ug |
$1,244.00
|
|
Plasmid Map for MG215873
Download sequence for
Cloning scheme for MG215873
Circular map for MG215873
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
- Price:
- Actual Price:
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