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Wdr27 (NM_175173) Mouse Tagged ORF Clone

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SKU
MG215852
Wdr27 (tGFP-tagged) - Mouse WD repeat domain 27 (Wdr27), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $914.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wdr27
Synonyms 0610012K18Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215852 representing NM_175173
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAACGCCTCCAGAGATGCCCTCCACTGATGGCCACACTCGAGCTATAGTTACAGAACGATACGTGG
TTGAGTCGAAGAAGTCTGCAGCTCATGTTCCACTGGCTTGCAGCACACAGCACTGTGCTTTCCCTTTAGA
TGGGAGCCGTCTCTGTGTGTGGAGCTCCAAGGACCCTTCTCACCAGCTTCTCACCCTACAAGGACACCAT
CAATTGATTACTGCTGTGGTTTTTGGAAATCAAATAGATCCACTTCTCCTCTGCTCTGCATCAGAGGATT
ATATTATAATGTGGAACGTGGCTGAATGCAGAGAGAAGACACTTAAAGGGCTGACTCCTCGAGGGACTAT
CCTGGGCTCCCTTTTACAAACAGTGTTGTGTTTGCGATTTAGTCTGGATGATCGTGCTATTGCAGTGTGT
GCTGGAAACAAAATATCTGTGATGGATGTGGAGAAGCAGTCTGTGCTGGTAGAGTTGAAGGGCCATCAAG
GCTCAGTGACAGCAGTGGAGTTCTGTCCCTGGCAAGCACACACCCTCATCTCTGTGTCTGAAGACCGAAG
CTTTAAGGTCTGGGACTTTTGTGTGGGATCCTTGATATACAGTTCATCCATTTTAACAGCTTACCCTCTC
CTAAATTTGCTTATCAATGAAGAAAACCAACAGCTGGTCACCGGCAGTGCTGATGGCCAGCTGTGGATCT
TTAGTTTGATGGAGGGACATCACTACCACTGTGTGGCACATGTGGACGTAAGGAAGAAAAGGGAGACTTT
CACCACCCGAAGAATGATGGCTGAGCAGTGCAGCCTGCCAGAAGACCATCAGTGCCGGTGCAGACATGAG
GCGGATAAAAGGGGAGAGGCTGAAGCAACATTCCCTATCCTGAGCCTGGCGCCCTGTGACCTCTGCCTGC
CGGATTCCCAGCGTGGAGCTTTTGCTTCTGAGTGCACCAAATGTCTATGGATTGGAAGCTCAACAGCTCT
ATTCATATTGAACCTGGCAAGCTTTGAATTGGAAGCCGCTTTACACTTCAAGGAATTTCAGAGCCTGAGC
GTCCAAGTTGCTGGGTCGTGTGCAATGGTGAGCGAGCCCATGAGCGCCAAGGCATTTTGCATGCTTTCAT
CTATGTTTGGGAGTAAGATTGCTGTATTGGAAATTGACTTGGCTGCTCTGCTCAGCACTCAGCAGTACCC
TCGGGCAGGAAAGGTGCTTTCTGTGCTGGCAAGCTCCTGTGTCCTGCCAACTTCTCCACTGTATTTTGGA
ATTATTAAGGAAAAATTTCCTAAGCTTGCCAATACCAAACAACATGCTGTGAAGAGTGTCGTGGAGGACA
GGCCCCTGGTTTTCCACACTAAGGTTAGGTCGTCCGGCTATACATTGGCACCACATATGGCTATGTTTTC
TCCAAAGACTAACATCAAACATCATAACAAAAGATCTTCAAAGTACAAGAACAATTACAAATGTAAAGAG
TGCTCTTTGGAGAATTTCCTACCCAGAAATCTCAGCAGACAGGTTGCTGTAGCCCAGAAGCCTGTTGCTG
TGAGCTGTCTGCAGTTCTCAGGGGATGGACAGAAGCTGGCCTGTGGCTTAGGTAACCACCTGTCCCTGGT
CTTCAACGCCAGCCTTTCAGGACCACCGGCTGCCTTTTCAGGTCATGATGGAGCTGTGAGCACTATCTGC
TGGAGCCATGACAAGAGGTGGCTGCTGTCTACAGGCCGAGACCGGACACTGAGGGTGTGGTCAGTTCACA
GGACAGAACTCATGCTGCTTTTGGCTCCAGATGCTTTTCCCAAGCCAGTAACATCTGCACAGTTCTACTA
CATGGACACCTTCATACTGCTGTCTTCTGGCCCAGAGTTCTGCCTGCTGAAGTACCACATTGACCTCTGT
AGGGATGACATACGAAGGTATAAACCAAAAAGCAGGTATAAGCCTATTTTCAGGTTGCCAATGACCAGTG
GGGCGGACATAACCAGCCTGTCAGCGGTGAACGACTTTTATTCCCACATCGTCCTCACAGCTGGCCGCGA
CCGGGCTGTTGAAGTTTTTGACCTCAATGCTGGCTGCAGCGCTGCAGTGCTTGCTGAAGTCCATTCCAGG
CCTGTTCATCGAATTTGTCAAAATAAAGGATCATCCTTTGTAGCCCAGCATTCCCTGGCATATAATCTTT
TCCTGACGGCAGCTGTTGGTGATGGGATTCGACTGTGGGACTTGAGGAACTTGAGGCTTATGTGTATGAA
ATGGGCTCCAGCAGCTTTCTGCACCGTCTTGCAGGACACACAGATACGATTACTGAAGTGGCCTTCAACC
CAGCAGCTCCTCAGCTTGTCACAGCCACCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215852 representing NM_175173
Red=Cloning site Green=Tags(s)

METPPEMPSTDGHTRAIVTERYVVESKKSAAHVPLACSTQHCAFPLDGSRLCVWSSKDPSHQLLTLQGHH
QLITAVVFGNQIDPLLLCSASEDYIIMWNVAECREKTLKGLTPRGTILGSLLQTVLCLRFSLDDRAIAVC
AGNKISVMDVEKQSVLVELKGHQGSVTAVEFCPWQAHTLISVSEDRSFKVWDFCVGSLIYSSSILTAYPL
LNLLINEENQQLVTGSADGQLWIFSLMEGHHYHCVAHVDVRKKRETFTTRRMMAEQCSLPEDHQCRCRHE
ADKRGEAEATFPILSLAPCDLCLPDSQRGAFASECTKCLWIGSSTALFILNLASFELEAALHFKEFQSLS
VQVAGSCAMVSEPMSAKAFCMLSSMFGSKIAVLEIDLAALLSTQQYPRAGKVLSVLASSCVLPTSPLYFG
IIKEKFPKLANTKQHAVKSVVEDRPLVFHTKVRSSGYTLAPHMAMFSPKTNIKHHNKRSSKYKNNYKCKE
CSLENFLPRNLSRQVAVAQKPVAVSCLQFSGDGQKLACGLGNHLSLVFNASLSGPPAAFSGHDGAVSTIC
WSHDKRWLLSTGRDRTLRVWSVHRTELMLLLAPDAFPKPVTSAQFYYMDTFILLSSGPEFCLLKYHIDLC
RDDIRRYKPKSRYKPIFRLPMTSGADITSLSAVNDFYSHIVLTAGRDRAVEVFDLNAGCSAAVLAEVHSR
PVHRICQNKGSSFVAQHSLAYNLFLTAAVGDGIRLWDLRNLRLMCMKWAPAAFCTVLQDTQIRLLKWPST
QQLLSLSQPP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175173
ORF Size 2340 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175173.3, NP_780382.2
RefSeq Size 2759 bp
RefSeq ORF 2343 bp
Locus ID 71682
UniProt ID Q8C5V5
Cytogenetics 17 A2
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Documents
Resources
"NM_175173" in other vectors (4)
SKU Description Size Price
MC221578 Wdr27 (untagged) - Mouse WD repeat domain 27 (Wdr27), (10ug) 10 ug
$787.00
MR215852 Wdr27 (Myc-DDK-tagged) - Mouse WD repeat domain 27 (Wdr27) 10 ug
$589.00 $714.00
MR215852L3 Lenti ORF clone of Wdr27 (Myc-DDK-tagged) - Mouse WD repeat domain 27 (Wdr27) 10 ug
$1,014.00
MR215852L4 Lenti ORF clone of Wdr27 (mGFP-tagged) - Mouse WD repeat domain 27 (Wdr27) 10 ug
$1,014.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.