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Cyth4 (NM_028195) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215825
Cyth4 (tGFP-tagged) - Mouse cytohesin 4 (Cyth4), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Cyth4 or NM_028195"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cyth4
Synonyms 2510004M07Rik; 5830469K17Rik; AI467541; mFLJ00017; Pscd4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215825 representing NM_028195
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGTGTGTCACACAGATCCAGCTGAGCTCAGCAGTGGGGAGGCCAAGGAGCTACAGCAGATCAAAT
GGCACAGGAAGCAGCTACTGGAAGACATCCAGAAACTGAAGGATGAGATTGCAGATGTGTTTGCCCAAAT
CGACTGCTTTGAAAGCACTGAGGAGAGCCGGATGGCGCAGAAAGAGAAGGAGATGTGCATCGGGAGAAAG
AAATTCAACATGGACCCTAATAAGGGCATCCAGTATCTCATTGAGCACAAGCTGCTGACCTCTGATGTCC
AGGACATCGCCCAGTTCCTGTACAAGGGTGACGGTCTCAACAAGACAGCCATTGGCACCTACTTGGGGGA
AAAGGACCCCATCAACTTGCAGGTCCTGCAGGCCTTTGTGGACTGCCATGAGTTTGCCAACCTCAACCTC
GTTCAGGCCCTCAGGCAGTTCCTGTGGAGCTTCAGGCTGCCGGGTGAGGCCCAGAAGATTGACCGCATGA
TGGAGGCCTTCGCTGCTCGCTACTGCCTCTGTAACCCCGGAGTCTTCCGGTCCACAGACACCTGCTACGT
GCTGTCCTTCTCCGTCATCATGCTCAACACCGGCCTACACAACCCCAACGTGCGGGACAGACCACCCTTC
GAGCGCTTTGTGACCATGAATCGCGGCATCAACAGCGGCAGTGACCTGCCCGAGGAGCAGCTACGGAACC
TGTTTGACAGCATCAAGAGTGAGCCCTTCTCCATCCCTGAGGATGATGGCGGTGACCTCACTCACACCTT
CTTTAACCCCGACCGTGAAGGCTGGCTACTCAAGCTGGGGGGCCGTGTGAAGACATGGAAGCGGCGCTGG
TTCATTCTCACTGACAACTGTCTCTACTACTTTGAGTTCACCACTGACAAAGAACCTCGGGGGATCATTC
CCCTGGAAAACCTCTCCGTGCAGAAGGTAGAGGACCCCAAGAAGCCGTTCTGCCTGGAGCTGTACAACCC
CAGCTGCCGGGGCCAGAAGATAAAGGCATGCAAGACAGACGGGGACGGCAAGGTGGTAGAAGGCAAACAT
GAATCCTACCGCATCTCAGCTGCCAATGCCGAGGAACGCGACCAGTGGATCGAGGCCATCAGGGCCAGCA
TCACCCGAGTCCCCTTCTACGACCTGTTGTCTGCTCGGAAAAAGAAGATTGTCGGCAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215825 representing NM_028195
Red=Cloning site Green=Tags(s)

MDVCHTDPAELSSGEAKELQQIKWHRKQLLEDIQKLKDEIADVFAQIDCFESTEESRMAQKEKEMCIGRK
KFNMDPNKGIQYLIEHKLLTSDVQDIAQFLYKGDGLNKTAIGTYLGEKDPINLQVLQAFVDCHEFANLNL
VQALRQFLWSFRLPGEAQKIDRMMEAFAARYCLCNPGVFRSTDTCYVLSFSVIMLNTGLHNPNVRDRPPF
ERFVTMNRGINSGSDLPEEQLRNLFDSIKSEPFSIPEDDGGDLTHTFFNPDREGWLLKLGGRVKTWKRRW
FILTDNCLYYFEFTTDKEPRGIIPLENLSVQKVEDPKKPFCLELYNPSCRGQKIKACKTDGDGKVVEGKH
ESYRISAANAEERDQWIEAIRASITRVPFYDLLSARKKKIVGK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028195
ORF Size 1179 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028195.3, NP_082471.2
RefSeq Size 2791 bp
RefSeq ORF 1182 bp
Locus ID 72318
UniProt ID Q80YW0
Cytogenetics 15 E1
Summary Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF factors through replacement of GDP with GTP (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028195" in other vectors (4)
SKU Description Size Price
MC211318 Cyth4 (untagged) - Mouse cytohesin 4 (Cyth4), (10ug) 10 ug
$503.00
MR215825 Cyth4 (Myc-DDK-tagged) - Mouse cytohesin 4 (Cyth4) 10 ug
$289.00 $457.00
MR215825L3 Lenti ORF clone of Cyth4 (Myc-DDK-tagged) - Mouse cytohesin 4 (Cyth4) 10 ug
$757.00
MR215825L4 Lenti ORF clone of Cyth4 (mGFP-tagged) - Mouse cytohesin 4 (Cyth4) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.